Effects of Phenylglyoxal and N-ethylmaleimide Concentration on Mycophenolic Acid Production by Penicillium brevi-compactum ATCC16024

Mycophenolic acid is a secondary extracellular metabolite of Penicillium strains with numerous pharmaceutical properties such as antibiotic and immunosuppressive uses. The aim of this work is the survey of the effect of phenylglyoxal and n-ethylmaleimide concentration in culture medium on mycophenolic acid production by Penicillium brevi-compactum ATCC16024 was investigated. Batch submerged fermentation was performed in 250 mL shake flasks at 24 °C and 200 rpm in a rotary shaker for 300 h using a basic culture medium containing different concentrations of phenylglyoxal and n-ethylmaleimide ranged from 0 to 20 mg. L(-1). For the basic medium without any amounts of phenylglyoxal and n-ethylmaleimide (control), maximum MPA production, product yield and productivity of process was in order, 1.5042 g. L(-1), 20.3 mg. g(-1) consumed glucose and 5.37 mg. L(-1)h(-1). Maximum produced MPA of 2.9032 g. L(-1), MPA yield of 39.23 mg. g(-1) of consumed glucose, productivity of 10.37 mg. L(-1) h(-1) and total enhancement of 93.11% was obtained when the culture medium was contained 18 mg. L(-1) of phenylglyoxal, represented more than 93% raising in compare to control. Maximum MPA concentration, yield and productivity in order was obtained 3.1123 g. L(-1), 42.06 mg. g(-1) of consumed glucose and 11.11 mg. L(-1) h(-1), with using 6 mg. L(-1) of n-ethylmaleimide. N-ethylmaleimide was caused to 2.138 folds (106.89%) increase in MPA production by P. brevi-compactum ATCC16024.