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Human skeletal muscle plasmalemma alters its structure to change its Ca(2+)-handling following heavy-load resistance exercise
High-force eccentric exercise results in sustained increases in cytoplasmic Ca(2+) levels ([Ca(2+)](cyto)), which can cause damage to the muscle. Here we report that a heavy-load strength training bout greatly alters the structure of the membrane network inside the fibres, the tubular (t-) system, c...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316829/ https://www.ncbi.nlm.nih.gov/pubmed/28193999 http://dx.doi.org/10.1038/ncomms14266 |
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author | Cully, Tanya R. Murphy, Robyn M. Roberts, Llion Raastad, Truls Fassett, Robert G. Coombes, Jeff S. Jayasinghe, Izzy Launikonis, Bradley S. |
author_facet | Cully, Tanya R. Murphy, Robyn M. Roberts, Llion Raastad, Truls Fassett, Robert G. Coombes, Jeff S. Jayasinghe, Izzy Launikonis, Bradley S. |
author_sort | Cully, Tanya R. |
collection | PubMed |
description | High-force eccentric exercise results in sustained increases in cytoplasmic Ca(2+) levels ([Ca(2+)](cyto)), which can cause damage to the muscle. Here we report that a heavy-load strength training bout greatly alters the structure of the membrane network inside the fibres, the tubular (t-) system, causing the loss of its predominantly transverse organization and an increase in vacuolation of its longitudinal tubules across adjacent sarcomeres. The transverse tubules and vacuoles displayed distinct Ca(2+)-handling properties. Both t-system components could take up Ca(2+) from the cytoplasm but only transverse tubules supported store-operated Ca(2+) entry. The retention of significant amounts of Ca(2+) within vacuoles provides an effective mechanism to reduce the total content of Ca(2+) within the fibre cytoplasm. We propose this ability can reduce or limit resistance exercise-induced, Ca(2+)-dependent damage to the fibre by the reduction of [Ca(2+)](cyto) to help maintain fibre viability during the period associated with delayed onset muscle soreness. |
format | Online Article Text |
id | pubmed-5316829 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53168292017-02-27 Human skeletal muscle plasmalemma alters its structure to change its Ca(2+)-handling following heavy-load resistance exercise Cully, Tanya R. Murphy, Robyn M. Roberts, Llion Raastad, Truls Fassett, Robert G. Coombes, Jeff S. Jayasinghe, Izzy Launikonis, Bradley S. Nat Commun Article High-force eccentric exercise results in sustained increases in cytoplasmic Ca(2+) levels ([Ca(2+)](cyto)), which can cause damage to the muscle. Here we report that a heavy-load strength training bout greatly alters the structure of the membrane network inside the fibres, the tubular (t-) system, causing the loss of its predominantly transverse organization and an increase in vacuolation of its longitudinal tubules across adjacent sarcomeres. The transverse tubules and vacuoles displayed distinct Ca(2+)-handling properties. Both t-system components could take up Ca(2+) from the cytoplasm but only transverse tubules supported store-operated Ca(2+) entry. The retention of significant amounts of Ca(2+) within vacuoles provides an effective mechanism to reduce the total content of Ca(2+) within the fibre cytoplasm. We propose this ability can reduce or limit resistance exercise-induced, Ca(2+)-dependent damage to the fibre by the reduction of [Ca(2+)](cyto) to help maintain fibre viability during the period associated with delayed onset muscle soreness. Nature Publishing Group 2017-02-13 /pmc/articles/PMC5316829/ /pubmed/28193999 http://dx.doi.org/10.1038/ncomms14266 Text en Copyright © 2017, The Author(s) https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) |
spellingShingle | Article Cully, Tanya R. Murphy, Robyn M. Roberts, Llion Raastad, Truls Fassett, Robert G. Coombes, Jeff S. Jayasinghe, Izzy Launikonis, Bradley S. Human skeletal muscle plasmalemma alters its structure to change its Ca(2+)-handling following heavy-load resistance exercise |
title | Human skeletal muscle plasmalemma alters its structure to change its
Ca(2+)-handling following heavy-load resistance exercise |
title_full | Human skeletal muscle plasmalemma alters its structure to change its
Ca(2+)-handling following heavy-load resistance exercise |
title_fullStr | Human skeletal muscle plasmalemma alters its structure to change its
Ca(2+)-handling following heavy-load resistance exercise |
title_full_unstemmed | Human skeletal muscle plasmalemma alters its structure to change its
Ca(2+)-handling following heavy-load resistance exercise |
title_short | Human skeletal muscle plasmalemma alters its structure to change its
Ca(2+)-handling following heavy-load resistance exercise |
title_sort | human skeletal muscle plasmalemma alters its structure to change its
ca(2+)-handling following heavy-load resistance exercise |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5316829/ https://www.ncbi.nlm.nih.gov/pubmed/28193999 http://dx.doi.org/10.1038/ncomms14266 |
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