Structural characterization of EasH (Aspergillus japonicus) – an oxidase involved in cycloclavine biosynthesis

Aj_EasH is a non-heme iron- and α-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. The three dimensional structure of Aj_EasH (2.2 Å resolution) reported here provides insight into the mechan...

Descripción completa

Detalles Bibliográficos
Autores principales: Jakubczyk, Dorota, Caputi, Lorenzo, Stevenson, Clare E. M., Lawson, David M., O'Connor, Sarah E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2016
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5317212/
https://www.ncbi.nlm.nih.gov/pubmed/27885368
http://dx.doi.org/10.1039/c6cc08438a
_version_ 1782508965322555392
author Jakubczyk, Dorota
Caputi, Lorenzo
Stevenson, Clare E. M.
Lawson, David M.
O'Connor, Sarah E.
author_facet Jakubczyk, Dorota
Caputi, Lorenzo
Stevenson, Clare E. M.
Lawson, David M.
O'Connor, Sarah E.
author_sort Jakubczyk, Dorota
collection PubMed
description Aj_EasH is a non-heme iron- and α-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. The three dimensional structure of Aj_EasH (2.2 Å resolution) reported here provides insight into the mechanism of this unusual and complex reaction.
format Online
Article
Text
id pubmed-5317212
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-53172122017-03-01 Structural characterization of EasH (Aspergillus japonicus) – an oxidase involved in cycloclavine biosynthesis Jakubczyk, Dorota Caputi, Lorenzo Stevenson, Clare E. M. Lawson, David M. O'Connor, Sarah E. Chem Commun (Camb) Chemistry Aj_EasH is a non-heme iron- and α-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. The three dimensional structure of Aj_EasH (2.2 Å resolution) reported here provides insight into the mechanism of this unusual and complex reaction. Royal Society of Chemistry 2016-12-25 2016-11-22 /pmc/articles/PMC5317212/ /pubmed/27885368 http://dx.doi.org/10.1039/c6cc08438a Text en This journal is © The Royal Society of Chemistry 2016 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Jakubczyk, Dorota
Caputi, Lorenzo
Stevenson, Clare E. M.
Lawson, David M.
O'Connor, Sarah E.
Structural characterization of EasH (Aspergillus japonicus) – an oxidase involved in cycloclavine biosynthesis
title Structural characterization of EasH (Aspergillus japonicus) – an oxidase involved in cycloclavine biosynthesis
title_full Structural characterization of EasH (Aspergillus japonicus) – an oxidase involved in cycloclavine biosynthesis
title_fullStr Structural characterization of EasH (Aspergillus japonicus) – an oxidase involved in cycloclavine biosynthesis
title_full_unstemmed Structural characterization of EasH (Aspergillus japonicus) – an oxidase involved in cycloclavine biosynthesis
title_short Structural characterization of EasH (Aspergillus japonicus) – an oxidase involved in cycloclavine biosynthesis
title_sort structural characterization of eash (aspergillus japonicus) – an oxidase involved in cycloclavine biosynthesis
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5317212/
https://www.ncbi.nlm.nih.gov/pubmed/27885368
http://dx.doi.org/10.1039/c6cc08438a
work_keys_str_mv AT jakubczykdorota structuralcharacterizationofeashaspergillusjaponicusanoxidaseinvolvedincycloclavinebiosynthesis
AT caputilorenzo structuralcharacterizationofeashaspergillusjaponicusanoxidaseinvolvedincycloclavinebiosynthesis
AT stevensonclareem structuralcharacterizationofeashaspergillusjaponicusanoxidaseinvolvedincycloclavinebiosynthesis
AT lawsondavidm structuralcharacterizationofeashaspergillusjaponicusanoxidaseinvolvedincycloclavinebiosynthesis
AT oconnorsarahe structuralcharacterizationofeashaspergillusjaponicusanoxidaseinvolvedincycloclavinebiosynthesis

Ejemplares similares