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Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage

BACKGROUND: Precise genome editing via homology-directed repair (HDR) after double-stranded DNA (dsDNA) cleavage facilitates functional genomic research and holds promise for gene therapy. However, HDR efficiency remains low in some cell types, including some of great research and clinical interest,...

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Detalles Bibliográficos
Autores principales:	Zhang, Jian-Ping, Li, Xiao-Lan, Li, Guo-Hua, Chen, Wanqiu, Arakaki, Cameron, Botimer, Gary D., Baylink, David, Zhang, Lu, Wen, Wei, Fu, Ya-Wen, Xu, Jing, Chun, Noah, Yuan, Weiping, Cheng, Tao, Zhang, Xiao-Bing
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5319046/ https://www.ncbi.nlm.nih.gov/pubmed/28219395 http://dx.doi.org/10.1186/s13059-017-1164-8

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