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P450(BM3) fused to phosphite dehydrogenase allows phosphite-driven selective oxidations
To facilitate the wider application of the NADPH-dependent P450(BM3,) we fused the monooxygenase with a phosphite dehydrogenase (PTDH). The resulting monooxygenase-dehydrogenase fusion enzyme acts as a self-sufficient bifunctional catalyst, accepting phosphite as a cheap electron donor for the regen...
Autores principales: | Beyer, Nina, Kulig, Justyna K., Bartsch, Anette, Hayes, Martin A., Janssen, Dick B., Fraaije, Marco W. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320008/ https://www.ncbi.nlm.nih.gov/pubmed/27900443 http://dx.doi.org/10.1007/s00253-016-7993-7 |
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