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Data on secondary structures and ligand interactions of G-rich oligonucleotides that defy the classical formula for G4 motifs
The data provided in this article are related to the research article "The expanding repertoire of G4 DNA structures" [1]. Secondary structures of G-rich oligonucleotides (ONs) that represent “imperfect” G-quadruplex (G4) motifs, i.e., contain truncated or interrupted G-runs, were analyzed...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320062/ https://www.ncbi.nlm.nih.gov/pubmed/28243622 http://dx.doi.org/10.1016/j.dib.2017.02.023 |
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author | Vlasenok, Maria Varizhuk, Anna Kaluzhny, Dmitry Smirnov, Igor Pozmogova, Galina |
author_facet | Vlasenok, Maria Varizhuk, Anna Kaluzhny, Dmitry Smirnov, Igor Pozmogova, Galina |
author_sort | Vlasenok, Maria |
collection | PubMed |
description | The data provided in this article are related to the research article "The expanding repertoire of G4 DNA structures" [1]. Secondary structures of G-rich oligonucleotides (ONs) that represent “imperfect” G-quadruplex (G4) motifs, i.e., contain truncated or interrupted G-runs, were analyzed by optical methods. Presented data on ON structures include circular dichroism (CD) spectra, thermal difference spectra (TDS) and UV -melting curves of the ONs; and rotational relaxation times (RRT) of ethidium bromide (EtBr) complexes with the ONs. TDS, CD spectra and UV-melting curves can be used to characterize the topologies and thermal stabilities of the ON structures. RRTs are roughly proportional to the hydrodynamic volumes of the complexes and thus can be used to distinguish between inter- and intramolecular ON structures. Presented data on ON interactions with small molecules include fluorescence emission spectra of the G4 sensor thioflavin T (ThT) in complexes with the ONs, and CD-melting curves of the ONs in the presence of G4-stabilizing ligands N-methylmesoporphyrin IX (NMM) and pyridostatin (PDS). These data should be useful for comparative analyses of classical G4s and “defective”G4s, such as quadruplexes with vacancies or bulges. |
format | Online Article Text |
id | pubmed-5320062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-53200622017-02-27 Data on secondary structures and ligand interactions of G-rich oligonucleotides that defy the classical formula for G4 motifs Vlasenok, Maria Varizhuk, Anna Kaluzhny, Dmitry Smirnov, Igor Pozmogova, Galina Data Brief Data Article The data provided in this article are related to the research article "The expanding repertoire of G4 DNA structures" [1]. Secondary structures of G-rich oligonucleotides (ONs) that represent “imperfect” G-quadruplex (G4) motifs, i.e., contain truncated or interrupted G-runs, were analyzed by optical methods. Presented data on ON structures include circular dichroism (CD) spectra, thermal difference spectra (TDS) and UV -melting curves of the ONs; and rotational relaxation times (RRT) of ethidium bromide (EtBr) complexes with the ONs. TDS, CD spectra and UV-melting curves can be used to characterize the topologies and thermal stabilities of the ON structures. RRTs are roughly proportional to the hydrodynamic volumes of the complexes and thus can be used to distinguish between inter- and intramolecular ON structures. Presented data on ON interactions with small molecules include fluorescence emission spectra of the G4 sensor thioflavin T (ThT) in complexes with the ONs, and CD-melting curves of the ONs in the presence of G4-stabilizing ligands N-methylmesoporphyrin IX (NMM) and pyridostatin (PDS). These data should be useful for comparative analyses of classical G4s and “defective”G4s, such as quadruplexes with vacancies or bulges. Elsevier 2017-02-12 /pmc/articles/PMC5320062/ /pubmed/28243622 http://dx.doi.org/10.1016/j.dib.2017.02.023 Text en © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Data Article Vlasenok, Maria Varizhuk, Anna Kaluzhny, Dmitry Smirnov, Igor Pozmogova, Galina Data on secondary structures and ligand interactions of G-rich oligonucleotides that defy the classical formula for G4 motifs |
title | Data on secondary structures and ligand interactions of G-rich oligonucleotides that defy the classical formula for G4 motifs |
title_full | Data on secondary structures and ligand interactions of G-rich oligonucleotides that defy the classical formula for G4 motifs |
title_fullStr | Data on secondary structures and ligand interactions of G-rich oligonucleotides that defy the classical formula for G4 motifs |
title_full_unstemmed | Data on secondary structures and ligand interactions of G-rich oligonucleotides that defy the classical formula for G4 motifs |
title_short | Data on secondary structures and ligand interactions of G-rich oligonucleotides that defy the classical formula for G4 motifs |
title_sort | data on secondary structures and ligand interactions of g-rich oligonucleotides that defy the classical formula for g4 motifs |
topic | Data Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320062/ https://www.ncbi.nlm.nih.gov/pubmed/28243622 http://dx.doi.org/10.1016/j.dib.2017.02.023 |
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