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Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer

Thyroid cancer is one of the most common types of cancer in endocrine system. In latest studies, harmine has been proved to inhibit the growth of several cancers in vitro and in vivo. In the current study, we evaluated the in vitro and in vivo anticancer efficiency of harmine against thyroid cancer...

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Detalles Bibliográficos
Autores principales: Ruan, Shu, Jia, Feng, Li, Jianbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320370/
https://www.ncbi.nlm.nih.gov/pubmed/28270853
http://dx.doi.org/10.1155/2017/9402615
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author Ruan, Shu
Jia, Feng
Li, Jianbo
author_facet Ruan, Shu
Jia, Feng
Li, Jianbo
author_sort Ruan, Shu
collection PubMed
description Thyroid cancer is one of the most common types of cancer in endocrine system. In latest studies, harmine has been proved to inhibit the growth of several cancers in vitro and in vivo. In the current study, we evaluated the in vitro and in vivo anticancer efficiency of harmine against thyroid cancer cell line TPC-1. The in vitro cytotoxicity of harmine evaluated by XTT assay indicated that harmine suppressed the proliferation of TPC-1 cells in a dose- and time-dependent manner. Moreover, harmine dose-dependently induced apoptosis of TPC-1 cells through regulating the ratio of Bcl-2/Bax. The colony forming ability of TPC-1 cells was also time-dependently inhibited by harmine. The inhibitory effects of harmine on migration and invasion of TPC-1 cells were studied by wound scratching and Transwell assays. In vivo evaluation showed that harmine effectively inhibited the growth of thyroid cancer in a dose-dependent manner in nude mice. Therefore, harmine might be a promising herbal medicine in the therapy of thyroid cancer and further efforts are needed to explore this therapeutic strategy.
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spelling pubmed-53203702017-03-07 Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer Ruan, Shu Jia, Feng Li, Jianbo Evid Based Complement Alternat Med Research Article Thyroid cancer is one of the most common types of cancer in endocrine system. In latest studies, harmine has been proved to inhibit the growth of several cancers in vitro and in vivo. In the current study, we evaluated the in vitro and in vivo anticancer efficiency of harmine against thyroid cancer cell line TPC-1. The in vitro cytotoxicity of harmine evaluated by XTT assay indicated that harmine suppressed the proliferation of TPC-1 cells in a dose- and time-dependent manner. Moreover, harmine dose-dependently induced apoptosis of TPC-1 cells through regulating the ratio of Bcl-2/Bax. The colony forming ability of TPC-1 cells was also time-dependently inhibited by harmine. The inhibitory effects of harmine on migration and invasion of TPC-1 cells were studied by wound scratching and Transwell assays. In vivo evaluation showed that harmine effectively inhibited the growth of thyroid cancer in a dose-dependent manner in nude mice. Therefore, harmine might be a promising herbal medicine in the therapy of thyroid cancer and further efforts are needed to explore this therapeutic strategy. Hindawi Publishing Corporation 2017 2017-02-08 /pmc/articles/PMC5320370/ /pubmed/28270853 http://dx.doi.org/10.1155/2017/9402615 Text en Copyright © 2017 Shu Ruan et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ruan, Shu
Jia, Feng
Li, Jianbo
Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer
title Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer
title_full Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer
title_fullStr Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer
title_full_unstemmed Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer
title_short Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer
title_sort potential antitumor effect of harmine in the treatment of thyroid cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320370/
https://www.ncbi.nlm.nih.gov/pubmed/28270853
http://dx.doi.org/10.1155/2017/9402615
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