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Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer
Thyroid cancer is one of the most common types of cancer in endocrine system. In latest studies, harmine has been proved to inhibit the growth of several cancers in vitro and in vivo. In the current study, we evaluated the in vitro and in vivo anticancer efficiency of harmine against thyroid cancer...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320370/ https://www.ncbi.nlm.nih.gov/pubmed/28270853 http://dx.doi.org/10.1155/2017/9402615 |
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author | Ruan, Shu Jia, Feng Li, Jianbo |
author_facet | Ruan, Shu Jia, Feng Li, Jianbo |
author_sort | Ruan, Shu |
collection | PubMed |
description | Thyroid cancer is one of the most common types of cancer in endocrine system. In latest studies, harmine has been proved to inhibit the growth of several cancers in vitro and in vivo. In the current study, we evaluated the in vitro and in vivo anticancer efficiency of harmine against thyroid cancer cell line TPC-1. The in vitro cytotoxicity of harmine evaluated by XTT assay indicated that harmine suppressed the proliferation of TPC-1 cells in a dose- and time-dependent manner. Moreover, harmine dose-dependently induced apoptosis of TPC-1 cells through regulating the ratio of Bcl-2/Bax. The colony forming ability of TPC-1 cells was also time-dependently inhibited by harmine. The inhibitory effects of harmine on migration and invasion of TPC-1 cells were studied by wound scratching and Transwell assays. In vivo evaluation showed that harmine effectively inhibited the growth of thyroid cancer in a dose-dependent manner in nude mice. Therefore, harmine might be a promising herbal medicine in the therapy of thyroid cancer and further efforts are needed to explore this therapeutic strategy. |
format | Online Article Text |
id | pubmed-5320370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-53203702017-03-07 Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer Ruan, Shu Jia, Feng Li, Jianbo Evid Based Complement Alternat Med Research Article Thyroid cancer is one of the most common types of cancer in endocrine system. In latest studies, harmine has been proved to inhibit the growth of several cancers in vitro and in vivo. In the current study, we evaluated the in vitro and in vivo anticancer efficiency of harmine against thyroid cancer cell line TPC-1. The in vitro cytotoxicity of harmine evaluated by XTT assay indicated that harmine suppressed the proliferation of TPC-1 cells in a dose- and time-dependent manner. Moreover, harmine dose-dependently induced apoptosis of TPC-1 cells through regulating the ratio of Bcl-2/Bax. The colony forming ability of TPC-1 cells was also time-dependently inhibited by harmine. The inhibitory effects of harmine on migration and invasion of TPC-1 cells were studied by wound scratching and Transwell assays. In vivo evaluation showed that harmine effectively inhibited the growth of thyroid cancer in a dose-dependent manner in nude mice. Therefore, harmine might be a promising herbal medicine in the therapy of thyroid cancer and further efforts are needed to explore this therapeutic strategy. Hindawi Publishing Corporation 2017 2017-02-08 /pmc/articles/PMC5320370/ /pubmed/28270853 http://dx.doi.org/10.1155/2017/9402615 Text en Copyright © 2017 Shu Ruan et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ruan, Shu Jia, Feng Li, Jianbo Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer |
title | Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer |
title_full | Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer |
title_fullStr | Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer |
title_full_unstemmed | Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer |
title_short | Potential Antitumor Effect of Harmine in the Treatment of Thyroid Cancer |
title_sort | potential antitumor effect of harmine in the treatment of thyroid cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320370/ https://www.ncbi.nlm.nih.gov/pubmed/28270853 http://dx.doi.org/10.1155/2017/9402615 |
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