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Human peripheral blood mononuclear cells (PBMCs) from smokers release higher levels of IL-1-like cytokines after exposure to combustion-generated ultrafine particles

Ultrafine particles (UFP) generated by combustion processes are often associated with adverse health effects. However, little is known about the inflammatory processes generated by UFP that may underlie their toxicological activity. Murine macrophages (J774.1 cells) and human peripheral blood mononu...

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Autores principales: De Falco, Gianluigi, Terlizzi, Michela, Sirignano, Mariano, Commodo, Mario, D’Anna, Andrea, Aquino, Rita P., Pinto, Aldo, Sorrentino, Rosalinda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320442/
https://www.ncbi.nlm.nih.gov/pubmed/28223692
http://dx.doi.org/10.1038/srep43016
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author De Falco, Gianluigi
Terlizzi, Michela
Sirignano, Mariano
Commodo, Mario
D’Anna, Andrea
Aquino, Rita P.
Pinto, Aldo
Sorrentino, Rosalinda
author_facet De Falco, Gianluigi
Terlizzi, Michela
Sirignano, Mariano
Commodo, Mario
D’Anna, Andrea
Aquino, Rita P.
Pinto, Aldo
Sorrentino, Rosalinda
author_sort De Falco, Gianluigi
collection PubMed
description Ultrafine particles (UFP) generated by combustion processes are often associated with adverse health effects. However, little is known about the inflammatory processes generated by UFP that may underlie their toxicological activity. Murine macrophages (J774.1 cells) and human peripheral blood mononuclear cells (PBMCs) were used to evaluate the molecular mechanism underlying the pro-inflammatory activity of UFP. The addition of soot particles to J774.1 cells induced a concentration-dependent release of IL-1α, IL-1β and IL-33 This effect was not associated with cell death and, in contrast to literature, was pronounced at very low concentrations (5–100 pg/ml). Similarly, UFP induced the release of IL-1α, IL-18 and IL-33 by PBMCs. However, this effect was solely observed in PBMCs obtained from smokers, as the PBMCs from non-smokers instead released higher levels of IL-10. The release of these cytokines after UFP exposure was caspase-1- and NLRP3 inflammasome-dependent in PBMCs from healthy smokers, whereas IL-1α release was calpain-dependent. These results show that UFP at very low concentrations are able to give rise to an inflammatory process that is responsible for IL-1α, IL-18 and IL-33 release, which is pronounced in PBMCs from smokers, confirming that these individuals are especially susceptible to inflammatory-based airway diseases once exposed to air pollution.
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spelling pubmed-53204422017-02-24 Human peripheral blood mononuclear cells (PBMCs) from smokers release higher levels of IL-1-like cytokines after exposure to combustion-generated ultrafine particles De Falco, Gianluigi Terlizzi, Michela Sirignano, Mariano Commodo, Mario D’Anna, Andrea Aquino, Rita P. Pinto, Aldo Sorrentino, Rosalinda Sci Rep Article Ultrafine particles (UFP) generated by combustion processes are often associated with adverse health effects. However, little is known about the inflammatory processes generated by UFP that may underlie their toxicological activity. Murine macrophages (J774.1 cells) and human peripheral blood mononuclear cells (PBMCs) were used to evaluate the molecular mechanism underlying the pro-inflammatory activity of UFP. The addition of soot particles to J774.1 cells induced a concentration-dependent release of IL-1α, IL-1β and IL-33 This effect was not associated with cell death and, in contrast to literature, was pronounced at very low concentrations (5–100 pg/ml). Similarly, UFP induced the release of IL-1α, IL-18 and IL-33 by PBMCs. However, this effect was solely observed in PBMCs obtained from smokers, as the PBMCs from non-smokers instead released higher levels of IL-10. The release of these cytokines after UFP exposure was caspase-1- and NLRP3 inflammasome-dependent in PBMCs from healthy smokers, whereas IL-1α release was calpain-dependent. These results show that UFP at very low concentrations are able to give rise to an inflammatory process that is responsible for IL-1α, IL-18 and IL-33 release, which is pronounced in PBMCs from smokers, confirming that these individuals are especially susceptible to inflammatory-based airway diseases once exposed to air pollution. Nature Publishing Group 2017-02-22 /pmc/articles/PMC5320442/ /pubmed/28223692 http://dx.doi.org/10.1038/srep43016 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
De Falco, Gianluigi
Terlizzi, Michela
Sirignano, Mariano
Commodo, Mario
D’Anna, Andrea
Aquino, Rita P.
Pinto, Aldo
Sorrentino, Rosalinda
Human peripheral blood mononuclear cells (PBMCs) from smokers release higher levels of IL-1-like cytokines after exposure to combustion-generated ultrafine particles
title Human peripheral blood mononuclear cells (PBMCs) from smokers release higher levels of IL-1-like cytokines after exposure to combustion-generated ultrafine particles
title_full Human peripheral blood mononuclear cells (PBMCs) from smokers release higher levels of IL-1-like cytokines after exposure to combustion-generated ultrafine particles
title_fullStr Human peripheral blood mononuclear cells (PBMCs) from smokers release higher levels of IL-1-like cytokines after exposure to combustion-generated ultrafine particles
title_full_unstemmed Human peripheral blood mononuclear cells (PBMCs) from smokers release higher levels of IL-1-like cytokines after exposure to combustion-generated ultrafine particles
title_short Human peripheral blood mononuclear cells (PBMCs) from smokers release higher levels of IL-1-like cytokines after exposure to combustion-generated ultrafine particles
title_sort human peripheral blood mononuclear cells (pbmcs) from smokers release higher levels of il-1-like cytokines after exposure to combustion-generated ultrafine particles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320442/
https://www.ncbi.nlm.nih.gov/pubmed/28223692
http://dx.doi.org/10.1038/srep43016
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