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Chemometric-assisted QuEChERS extraction method for post-harvest pesticide determination in fruits and vegetables

An effective analysis method was developed based on a chemometric tool for the simultaneous quantification of five different post-harvest pesticides (2,4-dichlorophenoxyacetic acid (2,4-D), carbendazim, thiabendazole, iprodione, and prochloraz) in fruits and vegetables. In the modified QuEChERS (qui...

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Autores principales: Li, Minmin, Dai, Chao, Wang, Fengzhong, Kong, Zhiqiang, He, Yan, Huang, Ya Tao, Fan, Bei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320482/
https://www.ncbi.nlm.nih.gov/pubmed/28225030
http://dx.doi.org/10.1038/srep42489
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author Li, Minmin
Dai, Chao
Wang, Fengzhong
Kong, Zhiqiang
He, Yan
Huang, Ya Tao
Fan, Bei
author_facet Li, Minmin
Dai, Chao
Wang, Fengzhong
Kong, Zhiqiang
He, Yan
Huang, Ya Tao
Fan, Bei
author_sort Li, Minmin
collection PubMed
description An effective analysis method was developed based on a chemometric tool for the simultaneous quantification of five different post-harvest pesticides (2,4-dichlorophenoxyacetic acid (2,4-D), carbendazim, thiabendazole, iprodione, and prochloraz) in fruits and vegetables. In the modified QuEChERS (quick, easy, cheap, effective, rugged and safe) method, the factors and responses for optimization of the extraction and cleanup analyses were compared using the Plackett–Burman (P–B) screening design. Furthermore, the significant factors (toluene percentage, hydrochloric acid (HCl) percentage, and graphitized carbon black (GCB) amount) were optimized using a central composite design (CCD) combined with Derringer’s desirability function (DF). The limits of quantification (LOQs) were estimated to be 1.0 μg/kg for 2,4-D, carbendazim, thiabendazole, and prochloraz, and 1.5 μg/kg for iprodione in food matrices. The mean recoveries were in the range of 70.4–113.9% with relative standard deviations (RSDs) of less than 16.9% at three spiking levels. The measurement uncertainty of the analytical method was determined using the bottom-up approach, which yielded an average value of 7.6%. Carbendazim was most frequently found in real samples analyzed using the developed method. Consequently, the analytical method can serve as an advantageous and rapid tool for determination of five preservative pesticides in fruits and vegetables.
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spelling pubmed-53204822017-02-24 Chemometric-assisted QuEChERS extraction method for post-harvest pesticide determination in fruits and vegetables Li, Minmin Dai, Chao Wang, Fengzhong Kong, Zhiqiang He, Yan Huang, Ya Tao Fan, Bei Sci Rep Article An effective analysis method was developed based on a chemometric tool for the simultaneous quantification of five different post-harvest pesticides (2,4-dichlorophenoxyacetic acid (2,4-D), carbendazim, thiabendazole, iprodione, and prochloraz) in fruits and vegetables. In the modified QuEChERS (quick, easy, cheap, effective, rugged and safe) method, the factors and responses for optimization of the extraction and cleanup analyses were compared using the Plackett–Burman (P–B) screening design. Furthermore, the significant factors (toluene percentage, hydrochloric acid (HCl) percentage, and graphitized carbon black (GCB) amount) were optimized using a central composite design (CCD) combined with Derringer’s desirability function (DF). The limits of quantification (LOQs) were estimated to be 1.0 μg/kg for 2,4-D, carbendazim, thiabendazole, and prochloraz, and 1.5 μg/kg for iprodione in food matrices. The mean recoveries were in the range of 70.4–113.9% with relative standard deviations (RSDs) of less than 16.9% at three spiking levels. The measurement uncertainty of the analytical method was determined using the bottom-up approach, which yielded an average value of 7.6%. Carbendazim was most frequently found in real samples analyzed using the developed method. Consequently, the analytical method can serve as an advantageous and rapid tool for determination of five preservative pesticides in fruits and vegetables. Nature Publishing Group 2017-02-22 /pmc/articles/PMC5320482/ /pubmed/28225030 http://dx.doi.org/10.1038/srep42489 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Li, Minmin
Dai, Chao
Wang, Fengzhong
Kong, Zhiqiang
He, Yan
Huang, Ya Tao
Fan, Bei
Chemometric-assisted QuEChERS extraction method for post-harvest pesticide determination in fruits and vegetables
title Chemometric-assisted QuEChERS extraction method for post-harvest pesticide determination in fruits and vegetables
title_full Chemometric-assisted QuEChERS extraction method for post-harvest pesticide determination in fruits and vegetables
title_fullStr Chemometric-assisted QuEChERS extraction method for post-harvest pesticide determination in fruits and vegetables
title_full_unstemmed Chemometric-assisted QuEChERS extraction method for post-harvest pesticide determination in fruits and vegetables
title_short Chemometric-assisted QuEChERS extraction method for post-harvest pesticide determination in fruits and vegetables
title_sort chemometric-assisted quechers extraction method for post-harvest pesticide determination in fruits and vegetables
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320482/
https://www.ncbi.nlm.nih.gov/pubmed/28225030
http://dx.doi.org/10.1038/srep42489
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