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Introduction and establishment of fluoroquinolone-resistant Shigella sonnei into Bhutan

Shigella sonnei is a major contributor to the global burden of diarrhoeal disease, generally associated with dysenteric diarrhoea in developed countries but also emerging in developing countries. The reason for the recent success of S. sonnei is unknown, but is likely catalysed by its ability to acq...

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Autores principales: Chung The, Hao, Rabaa, Maia A., Thanh, Duy Pham, Ruekit, Sirigade, Wangchuk, Sonam, Dorji, Tshering, Tshering, Kinzang Pem, Nguyen, To Nguyen Thi, Vinh, Phat Voong, Thanh, Tuyen Ha, Minh, Chau Nguyen Ngoc, Turner, Paul, Sar, Poda, Thwaites, Guy, Holt, Kathryn E., Thomson, Nicholas R., Bodhidatta, Ladaporn, Jeffries Mason, Carl, Baker, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320628/
https://www.ncbi.nlm.nih.gov/pubmed/28348825
http://dx.doi.org/10.1099/mgen.0.000042
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author Chung The, Hao
Rabaa, Maia A.
Thanh, Duy Pham
Ruekit, Sirigade
Wangchuk, Sonam
Dorji, Tshering
Tshering, Kinzang Pem
Nguyen, To Nguyen Thi
Vinh, Phat Voong
Thanh, Tuyen Ha
Minh, Chau Nguyen Ngoc
Turner, Paul
Sar, Poda
Thwaites, Guy
Holt, Kathryn E.
Thomson, Nicholas R.
Bodhidatta, Ladaporn
Jeffries Mason, Carl
Baker, Stephen
author_facet Chung The, Hao
Rabaa, Maia A.
Thanh, Duy Pham
Ruekit, Sirigade
Wangchuk, Sonam
Dorji, Tshering
Tshering, Kinzang Pem
Nguyen, To Nguyen Thi
Vinh, Phat Voong
Thanh, Tuyen Ha
Minh, Chau Nguyen Ngoc
Turner, Paul
Sar, Poda
Thwaites, Guy
Holt, Kathryn E.
Thomson, Nicholas R.
Bodhidatta, Ladaporn
Jeffries Mason, Carl
Baker, Stephen
author_sort Chung The, Hao
collection PubMed
description Shigella sonnei is a major contributor to the global burden of diarrhoeal disease, generally associated with dysenteric diarrhoea in developed countries but also emerging in developing countries. The reason for the recent success of S. sonnei is unknown, but is likely catalysed by its ability to acquire resistance against multiple antimicrobials. Between 2011 and 2013, S. sonnei exhibiting resistance to fluoroquinolones, the first-line treatment recommended for shigellosis, emerged in Bhutan. Aiming to reconstruct the introduction and establishment of fluoroquinolone-resistant S. sonnei populations in Bhutan, we performed whole-genome sequencing on 71 S. sonnei samples isolated in Bhutan between 2011 and 2013.We found that these strains represented an expansion of a clade within the previously described lineage III, found specifically in Central Asia. Temporal phylogenetic reconstruction demonstrated that all of the sequenced Bhutanese S. sonnei diverged from a single ancestor that was introduced into Bhutan around 2006. Our data additionally predicted that fluoroquinolone resistance, conferred by mutations in gyrA and parC, arose prior to the introduction of the founder strain into Bhutan. Once established in Bhutan, these S. sonnei had access to a broad gene pool, as indicated by the acquisition of extended-spectrum β-lactamase-encoding plasmids and genes encoding type IV pili. The data presented here outline a model for the introduction and maintenance of fluoroquinolone-resistant S. sonnei in a new setting. Given the current circulation of fluoroquinolone-resistant S. sonnei in Asia, we speculate that this pattern of introduction is being recapitulated across the region and beyond.
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spelling pubmed-53206282017-03-27 Introduction and establishment of fluoroquinolone-resistant Shigella sonnei into Bhutan Chung The, Hao Rabaa, Maia A. Thanh, Duy Pham Ruekit, Sirigade Wangchuk, Sonam Dorji, Tshering Tshering, Kinzang Pem Nguyen, To Nguyen Thi Vinh, Phat Voong Thanh, Tuyen Ha Minh, Chau Nguyen Ngoc Turner, Paul Sar, Poda Thwaites, Guy Holt, Kathryn E. Thomson, Nicholas R. Bodhidatta, Ladaporn Jeffries Mason, Carl Baker, Stephen Microb Genom Research Paper Shigella sonnei is a major contributor to the global burden of diarrhoeal disease, generally associated with dysenteric diarrhoea in developed countries but also emerging in developing countries. The reason for the recent success of S. sonnei is unknown, but is likely catalysed by its ability to acquire resistance against multiple antimicrobials. Between 2011 and 2013, S. sonnei exhibiting resistance to fluoroquinolones, the first-line treatment recommended for shigellosis, emerged in Bhutan. Aiming to reconstruct the introduction and establishment of fluoroquinolone-resistant S. sonnei populations in Bhutan, we performed whole-genome sequencing on 71 S. sonnei samples isolated in Bhutan between 2011 and 2013.We found that these strains represented an expansion of a clade within the previously described lineage III, found specifically in Central Asia. Temporal phylogenetic reconstruction demonstrated that all of the sequenced Bhutanese S. sonnei diverged from a single ancestor that was introduced into Bhutan around 2006. Our data additionally predicted that fluoroquinolone resistance, conferred by mutations in gyrA and parC, arose prior to the introduction of the founder strain into Bhutan. Once established in Bhutan, these S. sonnei had access to a broad gene pool, as indicated by the acquisition of extended-spectrum β-lactamase-encoding plasmids and genes encoding type IV pili. The data presented here outline a model for the introduction and maintenance of fluoroquinolone-resistant S. sonnei in a new setting. Given the current circulation of fluoroquinolone-resistant S. sonnei in Asia, we speculate that this pattern of introduction is being recapitulated across the region and beyond. Microbiology Society 2015-12-24 /pmc/articles/PMC5320628/ /pubmed/28348825 http://dx.doi.org/10.1099/mgen.0.000042 Text en © 2015 The Authors http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Research Paper
Chung The, Hao
Rabaa, Maia A.
Thanh, Duy Pham
Ruekit, Sirigade
Wangchuk, Sonam
Dorji, Tshering
Tshering, Kinzang Pem
Nguyen, To Nguyen Thi
Vinh, Phat Voong
Thanh, Tuyen Ha
Minh, Chau Nguyen Ngoc
Turner, Paul
Sar, Poda
Thwaites, Guy
Holt, Kathryn E.
Thomson, Nicholas R.
Bodhidatta, Ladaporn
Jeffries Mason, Carl
Baker, Stephen
Introduction and establishment of fluoroquinolone-resistant Shigella sonnei into Bhutan
title Introduction and establishment of fluoroquinolone-resistant Shigella sonnei into Bhutan
title_full Introduction and establishment of fluoroquinolone-resistant Shigella sonnei into Bhutan
title_fullStr Introduction and establishment of fluoroquinolone-resistant Shigella sonnei into Bhutan
title_full_unstemmed Introduction and establishment of fluoroquinolone-resistant Shigella sonnei into Bhutan
title_short Introduction and establishment of fluoroquinolone-resistant Shigella sonnei into Bhutan
title_sort introduction and establishment of fluoroquinolone-resistant shigella sonnei into bhutan
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320628/
https://www.ncbi.nlm.nih.gov/pubmed/28348825
http://dx.doi.org/10.1099/mgen.0.000042
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