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TLR4 rs41426344 increases susceptibility of rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) in a central south Chinese Han population

BACKGROUND: The aim of the study was to determine whether polymorphisms in toll-like receptor 4 (TLR4) confer susceptibility to rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) in a central south Chinese Han population. METHODS: Genotyping for six well studied polymorphisms (rs49867...

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Autores principales: Wang, Yan, Chen, Lianghui, Li, Fang, Bao, Meihua, Zeng, Jie, Xiang, Ju, Luo, Huaiqing, Li, Jianming, Tang, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320652/
https://www.ncbi.nlm.nih.gov/pubmed/28222760
http://dx.doi.org/10.1186/s12969-017-0137-5
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author Wang, Yan
Chen, Lianghui
Li, Fang
Bao, Meihua
Zeng, Jie
Xiang, Ju
Luo, Huaiqing
Li, Jianming
Tang, Liang
author_facet Wang, Yan
Chen, Lianghui
Li, Fang
Bao, Meihua
Zeng, Jie
Xiang, Ju
Luo, Huaiqing
Li, Jianming
Tang, Liang
author_sort Wang, Yan
collection PubMed
description BACKGROUND: The aim of the study was to determine whether polymorphisms in toll-like receptor 4 (TLR4) confer susceptibility to rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) in a central south Chinese Han population. METHODS: Genotyping for six well studied polymorphisms (rs4986790, rs4986791, rs10759932, rs41426344, rs11536889 and rs7873784) in TLR4 gene were conducted in 1074 unrelated patients with RA and 1692 healthy control subjects, as well as in 217 unrelated patients with JIA and 378 healthy control subjects using direct sequencing technique. Comparisons between cases and controls in alleles, genotypes and haplotypes were carried out using Fisher’s exact test. RESULTS: Significant genetic associations were detected between the 3’UTR rs41426344C and RA (p < 0.001, p (adj) < 0.001, OR = 2.24) and JIA (p < 0.001, p (adj) < 0.001, OR = 2.05). In addition, rs4986790G was found to be significantly associated with the susceptibility for RA (p = 0.005, p (adj) = 0.03, OR = 3.43), but not for JIA (p = 0.06, p (adj) = 0.36, OR = 2.65). Furthermore, significant increasing in the distributions of haplotypes H4 and H10 in RA (H4: p = 0.001, OR = 1.13; H10: p = 0.001, OR = 1.15) and JIA (H4: p = 0.04, OR = 2.06; H10: p = 0.02, OR = 2.47) were also found. Moreover, the frequency of rs41426344C significantly increased in RF-positive and anti-CCP positive subjects both in RA (RF(+): p <0.0001, OR = 2.33; anti-CCP(+): p =0.008, OR = 2.79) and JIA (RF(+): p =0.02, OR = 2.91; anti-CCP(+): p = 0.02, OR = 2.78). CONCLUSIONS: Our study suggested that rs41426344 and rs4986790 of TLR4 might contribute to RA, and rs41426344 might contribute to JIA pathogenesis in central south Chinese Han population. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12969-017-0137-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-53206522017-02-24 TLR4 rs41426344 increases susceptibility of rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) in a central south Chinese Han population Wang, Yan Chen, Lianghui Li, Fang Bao, Meihua Zeng, Jie Xiang, Ju Luo, Huaiqing Li, Jianming Tang, Liang Pediatr Rheumatol Online J Research Article BACKGROUND: The aim of the study was to determine whether polymorphisms in toll-like receptor 4 (TLR4) confer susceptibility to rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) in a central south Chinese Han population. METHODS: Genotyping for six well studied polymorphisms (rs4986790, rs4986791, rs10759932, rs41426344, rs11536889 and rs7873784) in TLR4 gene were conducted in 1074 unrelated patients with RA and 1692 healthy control subjects, as well as in 217 unrelated patients with JIA and 378 healthy control subjects using direct sequencing technique. Comparisons between cases and controls in alleles, genotypes and haplotypes were carried out using Fisher’s exact test. RESULTS: Significant genetic associations were detected between the 3’UTR rs41426344C and RA (p < 0.001, p (adj) < 0.001, OR = 2.24) and JIA (p < 0.001, p (adj) < 0.001, OR = 2.05). In addition, rs4986790G was found to be significantly associated with the susceptibility for RA (p = 0.005, p (adj) = 0.03, OR = 3.43), but not for JIA (p = 0.06, p (adj) = 0.36, OR = 2.65). Furthermore, significant increasing in the distributions of haplotypes H4 and H10 in RA (H4: p = 0.001, OR = 1.13; H10: p = 0.001, OR = 1.15) and JIA (H4: p = 0.04, OR = 2.06; H10: p = 0.02, OR = 2.47) were also found. Moreover, the frequency of rs41426344C significantly increased in RF-positive and anti-CCP positive subjects both in RA (RF(+): p <0.0001, OR = 2.33; anti-CCP(+): p =0.008, OR = 2.79) and JIA (RF(+): p =0.02, OR = 2.91; anti-CCP(+): p = 0.02, OR = 2.78). CONCLUSIONS: Our study suggested that rs41426344 and rs4986790 of TLR4 might contribute to RA, and rs41426344 might contribute to JIA pathogenesis in central south Chinese Han population. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12969-017-0137-5) contains supplementary material, which is available to authorized users. BioMed Central 2017-02-21 /pmc/articles/PMC5320652/ /pubmed/28222760 http://dx.doi.org/10.1186/s12969-017-0137-5 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wang, Yan
Chen, Lianghui
Li, Fang
Bao, Meihua
Zeng, Jie
Xiang, Ju
Luo, Huaiqing
Li, Jianming
Tang, Liang
TLR4 rs41426344 increases susceptibility of rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) in a central south Chinese Han population
title TLR4 rs41426344 increases susceptibility of rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) in a central south Chinese Han population
title_full TLR4 rs41426344 increases susceptibility of rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) in a central south Chinese Han population
title_fullStr TLR4 rs41426344 increases susceptibility of rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) in a central south Chinese Han population
title_full_unstemmed TLR4 rs41426344 increases susceptibility of rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) in a central south Chinese Han population
title_short TLR4 rs41426344 increases susceptibility of rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) in a central south Chinese Han population
title_sort tlr4 rs41426344 increases susceptibility of rheumatoid arthritis (ra) and juvenile idiopathic arthritis (jia) in a central south chinese han population
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320652/
https://www.ncbi.nlm.nih.gov/pubmed/28222760
http://dx.doi.org/10.1186/s12969-017-0137-5
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