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Experimental infections of different carp strains with the carp edema virus (CEV) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (KSD) in carp aquaculture
Outbreaks of koi sleepy disease (KSD) caused by carp edema virus (CEV) may seriously affect populations of farmed common carp, one of the most important fish species for global food production. The present study shows further evidence for the involvement of CEV in outbreaks of KSD among carp and koi...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320791/ https://www.ncbi.nlm.nih.gov/pubmed/28222784 http://dx.doi.org/10.1186/s13567-017-0416-7 |
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author | Adamek, Mikolaj Oschilewski, Anna Wohlsein, Peter Jung-Schroers, Verena Teitge, Felix Dawson, Andy Gela, David Piackova, Veronika Kocour, Martin Adamek, Jerzy Bergmann, Sven M. Steinhagen, Dieter |
author_facet | Adamek, Mikolaj Oschilewski, Anna Wohlsein, Peter Jung-Schroers, Verena Teitge, Felix Dawson, Andy Gela, David Piackova, Veronika Kocour, Martin Adamek, Jerzy Bergmann, Sven M. Steinhagen, Dieter |
author_sort | Adamek, Mikolaj |
collection | PubMed |
description | Outbreaks of koi sleepy disease (KSD) caused by carp edema virus (CEV) may seriously affect populations of farmed common carp, one of the most important fish species for global food production. The present study shows further evidence for the involvement of CEV in outbreaks of KSD among carp and koi populations: in a series of infection experiments, CEV from two different genogroups could be transmitted to several strains of naïve common carp via cohabitation with fish infected with CEV. In recipient fish, clinical signs of KSD were induced. The virus load and viral gene expression results confirm gills as the target organ for CEV replication. Gill explants also allowed for a limited virus replication in vitro. The in vivo infection experiments revealed differences in the virulence of the two CEV genogroups which were associated with infections in koi or in common carp, with higher virulence towards the same fish variety as the donor fish. When the susceptibility of different carp strains to a CEV infection and the development of KSD were experimentally investigated, Amur wild carp showed to be relatively more resistant to the infection and did not develop clinical signs for KSD. However, the resistance could not be related to a higher magnitude of type I IFN responses of affected tissues. Despite not having a mechanistic explanation for the resistance of Amur wild carp to KSD, we recommend using this carp strain in breeding programs to limit potential losses caused by CEV in aquaculture. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13567-017-0416-7) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5320791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-53207912017-02-24 Experimental infections of different carp strains with the carp edema virus (CEV) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (KSD) in carp aquaculture Adamek, Mikolaj Oschilewski, Anna Wohlsein, Peter Jung-Schroers, Verena Teitge, Felix Dawson, Andy Gela, David Piackova, Veronika Kocour, Martin Adamek, Jerzy Bergmann, Sven M. Steinhagen, Dieter Vet Res Research Article Outbreaks of koi sleepy disease (KSD) caused by carp edema virus (CEV) may seriously affect populations of farmed common carp, one of the most important fish species for global food production. The present study shows further evidence for the involvement of CEV in outbreaks of KSD among carp and koi populations: in a series of infection experiments, CEV from two different genogroups could be transmitted to several strains of naïve common carp via cohabitation with fish infected with CEV. In recipient fish, clinical signs of KSD were induced. The virus load and viral gene expression results confirm gills as the target organ for CEV replication. Gill explants also allowed for a limited virus replication in vitro. The in vivo infection experiments revealed differences in the virulence of the two CEV genogroups which were associated with infections in koi or in common carp, with higher virulence towards the same fish variety as the donor fish. When the susceptibility of different carp strains to a CEV infection and the development of KSD were experimentally investigated, Amur wild carp showed to be relatively more resistant to the infection and did not develop clinical signs for KSD. However, the resistance could not be related to a higher magnitude of type I IFN responses of affected tissues. Despite not having a mechanistic explanation for the resistance of Amur wild carp to KSD, we recommend using this carp strain in breeding programs to limit potential losses caused by CEV in aquaculture. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13567-017-0416-7) contains supplementary material, which is available to authorized users. BioMed Central 2017-02-21 2017 /pmc/articles/PMC5320791/ /pubmed/28222784 http://dx.doi.org/10.1186/s13567-017-0416-7 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Adamek, Mikolaj Oschilewski, Anna Wohlsein, Peter Jung-Schroers, Verena Teitge, Felix Dawson, Andy Gela, David Piackova, Veronika Kocour, Martin Adamek, Jerzy Bergmann, Sven M. Steinhagen, Dieter Experimental infections of different carp strains with the carp edema virus (CEV) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (KSD) in carp aquaculture |
title | Experimental infections of different carp strains with the carp edema virus (CEV) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (KSD) in carp aquaculture |
title_full | Experimental infections of different carp strains with the carp edema virus (CEV) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (KSD) in carp aquaculture |
title_fullStr | Experimental infections of different carp strains with the carp edema virus (CEV) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (KSD) in carp aquaculture |
title_full_unstemmed | Experimental infections of different carp strains with the carp edema virus (CEV) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (KSD) in carp aquaculture |
title_short | Experimental infections of different carp strains with the carp edema virus (CEV) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (KSD) in carp aquaculture |
title_sort | experimental infections of different carp strains with the carp edema virus (cev) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (ksd) in carp aquaculture |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320791/ https://www.ncbi.nlm.nih.gov/pubmed/28222784 http://dx.doi.org/10.1186/s13567-017-0416-7 |
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