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Enzymatic Resistance of Corneas Crosslinked Using Riboflavin in Conjunction With Low Energy, High Energy, and Pulsed UVA Irradiation Modes
PURPOSE: To investigate the effect of various riboflavin/ultraviolet light (UVA) crosslinking (CXL) protocols on corneal enzymatic resistance. METHODS: A total of 66 enucleated porcine eyes, with the corneal epithelium removed, were divided into 6 groups. Group 1 remained untreated. Groups 2 to 6 re...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Association for Research in Vision and Ophthalmology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5321166/ https://www.ncbi.nlm.nih.gov/pubmed/27046119 http://dx.doi.org/10.1167/iovs.15-18769 |
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author | Aldahlawi, Nada H. Hayes, Sally O'Brart, David P. S. Akhbanbetova, Alina Littlechild, Stacy L. Meek, Keith M. |
author_facet | Aldahlawi, Nada H. Hayes, Sally O'Brart, David P. S. Akhbanbetova, Alina Littlechild, Stacy L. Meek, Keith M. |
author_sort | Aldahlawi, Nada H. |
collection | PubMed |
description | PURPOSE: To investigate the effect of various riboflavin/ultraviolet light (UVA) crosslinking (CXL) protocols on corneal enzymatic resistance. METHODS: A total of 66 enucleated porcine eyes, with the corneal epithelium removed, were divided into 6 groups. Group 1 remained untreated. Groups 2 to 6 received riboflavin/dextran for 30 minutes. Group 3 underwent standard CXL (SCXL) with 3 mW/cm(2) UVA for 30 minutes (total energy dose 5.4 J/cm(2)). Groups 4 and 5 underwent high intensity CXL (HCXL) using 30 mW/cm(2) UVA for 3 minutes (5.4 J/cm(2)) and 30 mW/cm(2) for 4 minutes (7.2 J/cm(2)), respectively. Group 6 was exposed to 8 minutes of 30 mW/cm(2) UVA in a 10-second on/10-second off pulsed-radiation mode (p-HCXL; 7.2 J/cm(2)). A central 8-mm disk from each cornea was submerged in pepsin digest solution at 23°C and measured daily. After 13 days, the dry weight was recorded from 5 samples in each group. RESULTS: The CXL-treated corneas took longer to digest than nonirradiated corneas (P < 0.0001). Differences in digestion time also were observed between CXL groups, such that, HCXL (5.4 J/cm(2)) < SCXL (5.4 J/cm(2)) < HCXL (7.2 J/cm(2)) < p-HCXL (7.2 J/cm(2); P < 0.0001). The dry weight of the SCXL (5.4 J/cm(2)) group was higher than the HCXL (5.4 and 7.2 J/cm(2); P < 0.001) and p-HCXL 7.2 J/cm(2) (P <0.05) groups. No difference was detected between the HCXL and p-HCXL 7.2 J/cm(2) groups. CONCLUSIONS: The intensity and distribution of the crosslinks formed within the cornea vary with different UVA protocols. The precise location and amount of crosslinking needed to prevent disease progression is unknown. |
format | Online Article Text |
id | pubmed-5321166 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Association for Research in Vision and Ophthalmology |
record_format | MEDLINE/PubMed |
spelling | pubmed-53211662017-02-27 Enzymatic Resistance of Corneas Crosslinked Using Riboflavin in Conjunction With Low Energy, High Energy, and Pulsed UVA Irradiation Modes Aldahlawi, Nada H. Hayes, Sally O'Brart, David P. S. Akhbanbetova, Alina Littlechild, Stacy L. Meek, Keith M. Invest Ophthalmol Vis Sci Cornea PURPOSE: To investigate the effect of various riboflavin/ultraviolet light (UVA) crosslinking (CXL) protocols on corneal enzymatic resistance. METHODS: A total of 66 enucleated porcine eyes, with the corneal epithelium removed, were divided into 6 groups. Group 1 remained untreated. Groups 2 to 6 received riboflavin/dextran for 30 minutes. Group 3 underwent standard CXL (SCXL) with 3 mW/cm(2) UVA for 30 minutes (total energy dose 5.4 J/cm(2)). Groups 4 and 5 underwent high intensity CXL (HCXL) using 30 mW/cm(2) UVA for 3 minutes (5.4 J/cm(2)) and 30 mW/cm(2) for 4 minutes (7.2 J/cm(2)), respectively. Group 6 was exposed to 8 minutes of 30 mW/cm(2) UVA in a 10-second on/10-second off pulsed-radiation mode (p-HCXL; 7.2 J/cm(2)). A central 8-mm disk from each cornea was submerged in pepsin digest solution at 23°C and measured daily. After 13 days, the dry weight was recorded from 5 samples in each group. RESULTS: The CXL-treated corneas took longer to digest than nonirradiated corneas (P < 0.0001). Differences in digestion time also were observed between CXL groups, such that, HCXL (5.4 J/cm(2)) < SCXL (5.4 J/cm(2)) < HCXL (7.2 J/cm(2)) < p-HCXL (7.2 J/cm(2); P < 0.0001). The dry weight of the SCXL (5.4 J/cm(2)) group was higher than the HCXL (5.4 and 7.2 J/cm(2); P < 0.001) and p-HCXL 7.2 J/cm(2) (P <0.05) groups. No difference was detected between the HCXL and p-HCXL 7.2 J/cm(2) groups. CONCLUSIONS: The intensity and distribution of the crosslinks formed within the cornea vary with different UVA protocols. The precise location and amount of crosslinking needed to prevent disease progression is unknown. The Association for Research in Vision and Ophthalmology 2016-04-05 2016-04 /pmc/articles/PMC5321166/ /pubmed/27046119 http://dx.doi.org/10.1167/iovs.15-18769 Text en http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. |
spellingShingle | Cornea Aldahlawi, Nada H. Hayes, Sally O'Brart, David P. S. Akhbanbetova, Alina Littlechild, Stacy L. Meek, Keith M. Enzymatic Resistance of Corneas Crosslinked Using Riboflavin in Conjunction With Low Energy, High Energy, and Pulsed UVA Irradiation Modes |
title | Enzymatic Resistance of Corneas Crosslinked Using Riboflavin in Conjunction With Low Energy, High Energy, and Pulsed UVA Irradiation Modes |
title_full | Enzymatic Resistance of Corneas Crosslinked Using Riboflavin in Conjunction With Low Energy, High Energy, and Pulsed UVA Irradiation Modes |
title_fullStr | Enzymatic Resistance of Corneas Crosslinked Using Riboflavin in Conjunction With Low Energy, High Energy, and Pulsed UVA Irradiation Modes |
title_full_unstemmed | Enzymatic Resistance of Corneas Crosslinked Using Riboflavin in Conjunction With Low Energy, High Energy, and Pulsed UVA Irradiation Modes |
title_short | Enzymatic Resistance of Corneas Crosslinked Using Riboflavin in Conjunction With Low Energy, High Energy, and Pulsed UVA Irradiation Modes |
title_sort | enzymatic resistance of corneas crosslinked using riboflavin in conjunction with low energy, high energy, and pulsed uva irradiation modes |
topic | Cornea |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5321166/ https://www.ncbi.nlm.nih.gov/pubmed/27046119 http://dx.doi.org/10.1167/iovs.15-18769 |
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