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Heat-induced-radiolabeling and click chemistry: A powerful combination for generating multifunctional nanomaterials
A key advantage of nanomaterials for biomedical applications is their ability to feature multiple small reporter groups (multimodality), or combinations of reporter groups and therapeutic agents (multifunctionality), while being targeted to cell surface receptors. Here a facile combination of techni...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5321420/ https://www.ncbi.nlm.nih.gov/pubmed/28225818 http://dx.doi.org/10.1371/journal.pone.0172722 |
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author | Yuan, Hushan Wilks, Moses Q. El Fakhri, Georges Normandin, Marc D. Kaittanis, Charalambos Josephson, Lee |
author_facet | Yuan, Hushan Wilks, Moses Q. El Fakhri, Georges Normandin, Marc D. Kaittanis, Charalambos Josephson, Lee |
author_sort | Yuan, Hushan |
collection | PubMed |
description | A key advantage of nanomaterials for biomedical applications is their ability to feature multiple small reporter groups (multimodality), or combinations of reporter groups and therapeutic agents (multifunctionality), while being targeted to cell surface receptors. Here a facile combination of techniques for the syntheses of multimodal, targeted nanoparticles (NPs) is presented, whereby heat-induced-radiolabeling (HIR) labels NPs with radiometals and so-called click chemistry is used to attach bioactive groups to the NP surface. Click-reactive alkyne or azide groups were first attached to the nonradioactive clinical Feraheme (FH) NPs. Resulting “Alkyne-FH” and “Azide-FH” intermediates, like the parent NP, tolerated (89)Zr labeling by the HIR method previously described. Subsequently, biomolecules were quickly conjugated to the radioactive NPs by either copper-catalyzed or copper-free click reactions with high efficiency. Synthesis of the Alkyne-FH or Azide-FH intermediates, followed by HIR and then by click reactions for biomolecule attachment, provides a simple and potentially general path for the synthesis of multimodal, multifunctional, and targeted NPs for biomedical applications. |
format | Online Article Text |
id | pubmed-5321420 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53214202017-03-09 Heat-induced-radiolabeling and click chemistry: A powerful combination for generating multifunctional nanomaterials Yuan, Hushan Wilks, Moses Q. El Fakhri, Georges Normandin, Marc D. Kaittanis, Charalambos Josephson, Lee PLoS One Research Article A key advantage of nanomaterials for biomedical applications is their ability to feature multiple small reporter groups (multimodality), or combinations of reporter groups and therapeutic agents (multifunctionality), while being targeted to cell surface receptors. Here a facile combination of techniques for the syntheses of multimodal, targeted nanoparticles (NPs) is presented, whereby heat-induced-radiolabeling (HIR) labels NPs with radiometals and so-called click chemistry is used to attach bioactive groups to the NP surface. Click-reactive alkyne or azide groups were first attached to the nonradioactive clinical Feraheme (FH) NPs. Resulting “Alkyne-FH” and “Azide-FH” intermediates, like the parent NP, tolerated (89)Zr labeling by the HIR method previously described. Subsequently, biomolecules were quickly conjugated to the radioactive NPs by either copper-catalyzed or copper-free click reactions with high efficiency. Synthesis of the Alkyne-FH or Azide-FH intermediates, followed by HIR and then by click reactions for biomolecule attachment, provides a simple and potentially general path for the synthesis of multimodal, multifunctional, and targeted NPs for biomedical applications. Public Library of Science 2017-02-22 /pmc/articles/PMC5321420/ /pubmed/28225818 http://dx.doi.org/10.1371/journal.pone.0172722 Text en © 2017 Yuan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Yuan, Hushan Wilks, Moses Q. El Fakhri, Georges Normandin, Marc D. Kaittanis, Charalambos Josephson, Lee Heat-induced-radiolabeling and click chemistry: A powerful combination for generating multifunctional nanomaterials |
title | Heat-induced-radiolabeling and click chemistry: A powerful combination for generating multifunctional nanomaterials |
title_full | Heat-induced-radiolabeling and click chemistry: A powerful combination for generating multifunctional nanomaterials |
title_fullStr | Heat-induced-radiolabeling and click chemistry: A powerful combination for generating multifunctional nanomaterials |
title_full_unstemmed | Heat-induced-radiolabeling and click chemistry: A powerful combination for generating multifunctional nanomaterials |
title_short | Heat-induced-radiolabeling and click chemistry: A powerful combination for generating multifunctional nanomaterials |
title_sort | heat-induced-radiolabeling and click chemistry: a powerful combination for generating multifunctional nanomaterials |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5321420/ https://www.ncbi.nlm.nih.gov/pubmed/28225818 http://dx.doi.org/10.1371/journal.pone.0172722 |
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