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Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm
BACKGROUND: Atrial fibrillation (AF) is the most common arrhythmia and despite obvious clinical importance remains its pathogenesis only partially explained. A relation between inflammation and AF has been suggested by findings of increased inflammatory markers in AF patients. OBJECTIVE: The goal of...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5321459/ https://www.ncbi.nlm.nih.gov/pubmed/28225836 http://dx.doi.org/10.1371/journal.pone.0172691 |
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author | Smorodinova, Natalia Bláha, Martin Melenovský, Vojtěch Rozsívalová, Karolína Přidal, Jaromír Ďurišová, Mária Pirk, Jan Kautzner, Josef Kučera, Tomáš |
author_facet | Smorodinova, Natalia Bláha, Martin Melenovský, Vojtěch Rozsívalová, Karolína Přidal, Jaromír Ďurišová, Mária Pirk, Jan Kautzner, Josef Kučera, Tomáš |
author_sort | Smorodinova, Natalia |
collection | PubMed |
description | BACKGROUND: Atrial fibrillation (AF) is the most common arrhythmia and despite obvious clinical importance remains its pathogenesis only partially explained. A relation between inflammation and AF has been suggested by findings of increased inflammatory markers in AF patients. OBJECTIVE: The goal of this study was to characterize morphologically and functionally CD45-positive inflammatory cell populations in atrial myocardium of patients with AF as compared to sinus rhythm (SR). METHODS: We examined 46 subjects (19 with AF, and 27 in SR) undergoing coronary bypass or valve surgery. Peroperative bioptic samples of the left and the right atrial tissue were examined using immunohistochemistry. RESULTS: The number of CD3+ T-lymphocytes and CD68-KP1+ cells were elevated in the left atrial myocardium of patients with AF compared to those in SR. Immune cell infiltration of LA was related to the rhythm, but not to age, body size, LA size, mitral regurgitation grade, type of surgery, systemic markers of inflammation or presence of diabetes or hypertension. Most of CD68-KP1+ cells corresponded to dendritic cell population based on their morphology and immunoreactivity for DC-SIGN. The numbers of mast cells and CD20+ B-lymphocytes did not differ between AF and SR patients. No foci of inflammation were detected in any sample. CONCLUSIONS: An immunohistochemical analysis of samples from patients undergoing open heart surgery showed moderate and site-specific increase of inflammatory cells in the atrial myocardium of patients with AF compared to those in SR, with prevailing population of monocyte-macrophage lineage. These cells and their cytokine products may play a role in atrial remodeling and AF persistence. |
format | Online Article Text |
id | pubmed-5321459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53214592017-03-09 Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm Smorodinova, Natalia Bláha, Martin Melenovský, Vojtěch Rozsívalová, Karolína Přidal, Jaromír Ďurišová, Mária Pirk, Jan Kautzner, Josef Kučera, Tomáš PLoS One Research Article BACKGROUND: Atrial fibrillation (AF) is the most common arrhythmia and despite obvious clinical importance remains its pathogenesis only partially explained. A relation between inflammation and AF has been suggested by findings of increased inflammatory markers in AF patients. OBJECTIVE: The goal of this study was to characterize morphologically and functionally CD45-positive inflammatory cell populations in atrial myocardium of patients with AF as compared to sinus rhythm (SR). METHODS: We examined 46 subjects (19 with AF, and 27 in SR) undergoing coronary bypass or valve surgery. Peroperative bioptic samples of the left and the right atrial tissue were examined using immunohistochemistry. RESULTS: The number of CD3+ T-lymphocytes and CD68-KP1+ cells were elevated in the left atrial myocardium of patients with AF compared to those in SR. Immune cell infiltration of LA was related to the rhythm, but not to age, body size, LA size, mitral regurgitation grade, type of surgery, systemic markers of inflammation or presence of diabetes or hypertension. Most of CD68-KP1+ cells corresponded to dendritic cell population based on their morphology and immunoreactivity for DC-SIGN. The numbers of mast cells and CD20+ B-lymphocytes did not differ between AF and SR patients. No foci of inflammation were detected in any sample. CONCLUSIONS: An immunohistochemical analysis of samples from patients undergoing open heart surgery showed moderate and site-specific increase of inflammatory cells in the atrial myocardium of patients with AF compared to those in SR, with prevailing population of monocyte-macrophage lineage. These cells and their cytokine products may play a role in atrial remodeling and AF persistence. Public Library of Science 2017-02-22 /pmc/articles/PMC5321459/ /pubmed/28225836 http://dx.doi.org/10.1371/journal.pone.0172691 Text en © 2017 Smorodinova et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Smorodinova, Natalia Bláha, Martin Melenovský, Vojtěch Rozsívalová, Karolína Přidal, Jaromír Ďurišová, Mária Pirk, Jan Kautzner, Josef Kučera, Tomáš Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm |
title | Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm |
title_full | Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm |
title_fullStr | Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm |
title_full_unstemmed | Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm |
title_short | Analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm |
title_sort | analysis of immune cell populations in atrial myocardium of patients with atrial fibrillation or sinus rhythm |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5321459/ https://www.ncbi.nlm.nih.gov/pubmed/28225836 http://dx.doi.org/10.1371/journal.pone.0172691 |
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