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Infant milk formulas differ regarding their allergenic activity and induction of T‐cell and cytokine responses

BACKGROUND: Several hydrolyzed cow's milk (CM) formulas are available for avoidance of allergic reactions in CM‐allergic children and for prevention of allergy development in high‐risk infants. Our aim was to compare CM formulas regarding the presence of immunoreactive CM components, IgE reacti...

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Autores principales: Hochwallner, H., Schulmeister, U., Swoboda, I., Focke‐Tejkl, M., Reininger, R., Civaj, V., Campana, R., Thalhamer, J., Scheiblhofer, S., Balic, N., Horak, F., Ollert, M., Papadopoulos, N. G., Quirce, S., Szepfalusi, Z., Herz, U., van Tol, E. A. F., Spitzauer, S., Valenta, R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5321598/
https://www.ncbi.nlm.nih.gov/pubmed/27455132
http://dx.doi.org/10.1111/all.12992
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author Hochwallner, H.
Schulmeister, U.
Swoboda, I.
Focke‐Tejkl, M.
Reininger, R.
Civaj, V.
Campana, R.
Thalhamer, J.
Scheiblhofer, S.
Balic, N.
Horak, F.
Ollert, M.
Papadopoulos, N. G.
Quirce, S.
Szepfalusi, Z.
Herz, U.
van Tol, E. A. F.
Spitzauer, S.
Valenta, R.
author_facet Hochwallner, H.
Schulmeister, U.
Swoboda, I.
Focke‐Tejkl, M.
Reininger, R.
Civaj, V.
Campana, R.
Thalhamer, J.
Scheiblhofer, S.
Balic, N.
Horak, F.
Ollert, M.
Papadopoulos, N. G.
Quirce, S.
Szepfalusi, Z.
Herz, U.
van Tol, E. A. F.
Spitzauer, S.
Valenta, R.
author_sort Hochwallner, H.
collection PubMed
description BACKGROUND: Several hydrolyzed cow's milk (CM) formulas are available for avoidance of allergic reactions in CM‐allergic children and for prevention of allergy development in high‐risk infants. Our aim was to compare CM formulas regarding the presence of immunoreactive CM components, IgE reactivity, allergenic activity, ability to induce T‐cell proliferation, and cytokine secretion. METHODS: A blinded analysis of eight CM formulas, one nonhydrolyzed, two partially hydrolyzed (PH), four extensively hydrolyzed (EH), and one amino acid formula, using biochemical techniques and specific antibody probes was conducted. IgE reactivity and allergenic activity of the formulas were tested with sera from CM‐allergic patients (n = 26) in RAST‐based assays and with rat basophils transfected with the human FcεRI, respectively. The induction of T‐cell proliferation and the secretion of cytokines in Peripheral blood mononuclear cell (PBMC) culture from CM allergic patients and nonallergic individuals were assessed. RESULTS: Immune‐reactive α‐lactalbumin and β‐lactoglobulin were found in the two PH formulas and casein components in one of the EH formulas. One PH formula and the EH formula containing casein components showed remaining IgE reactivity, whereas the other hydrolyzed formulas lacked IgE reactivity. Only two EH formulas and the amino acid formula did not induce T‐cell proliferation and proinflammatory cytokine release. The remaining formulas varied regarding the induction of Th2, Th1, and proinflammatory cytokines. CONCLUSION: Our results show that certain CM formulas without allergenic and low proinflammatory properties can be identified and they may also explain different outcomes obtained in clinical studies using CM formulas.
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spelling pubmed-53215982017-03-01 Infant milk formulas differ regarding their allergenic activity and induction of T‐cell and cytokine responses Hochwallner, H. Schulmeister, U. Swoboda, I. Focke‐Tejkl, M. Reininger, R. Civaj, V. Campana, R. Thalhamer, J. Scheiblhofer, S. Balic, N. Horak, F. Ollert, M. Papadopoulos, N. G. Quirce, S. Szepfalusi, Z. Herz, U. van Tol, E. A. F. Spitzauer, S. Valenta, R. Allergy ORIGINAL ARTICLES BACKGROUND: Several hydrolyzed cow's milk (CM) formulas are available for avoidance of allergic reactions in CM‐allergic children and for prevention of allergy development in high‐risk infants. Our aim was to compare CM formulas regarding the presence of immunoreactive CM components, IgE reactivity, allergenic activity, ability to induce T‐cell proliferation, and cytokine secretion. METHODS: A blinded analysis of eight CM formulas, one nonhydrolyzed, two partially hydrolyzed (PH), four extensively hydrolyzed (EH), and one amino acid formula, using biochemical techniques and specific antibody probes was conducted. IgE reactivity and allergenic activity of the formulas were tested with sera from CM‐allergic patients (n = 26) in RAST‐based assays and with rat basophils transfected with the human FcεRI, respectively. The induction of T‐cell proliferation and the secretion of cytokines in Peripheral blood mononuclear cell (PBMC) culture from CM allergic patients and nonallergic individuals were assessed. RESULTS: Immune‐reactive α‐lactalbumin and β‐lactoglobulin were found in the two PH formulas and casein components in one of the EH formulas. One PH formula and the EH formula containing casein components showed remaining IgE reactivity, whereas the other hydrolyzed formulas lacked IgE reactivity. Only two EH formulas and the amino acid formula did not induce T‐cell proliferation and proinflammatory cytokine release. The remaining formulas varied regarding the induction of Th2, Th1, and proinflammatory cytokines. CONCLUSION: Our results show that certain CM formulas without allergenic and low proinflammatory properties can be identified and they may also explain different outcomes obtained in clinical studies using CM formulas. John Wiley and Sons Inc. 2016-09-18 2017-03 /pmc/articles/PMC5321598/ /pubmed/27455132 http://dx.doi.org/10.1111/all.12992 Text en © 2016 The Authors. Allergy Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle ORIGINAL ARTICLES
Hochwallner, H.
Schulmeister, U.
Swoboda, I.
Focke‐Tejkl, M.
Reininger, R.
Civaj, V.
Campana, R.
Thalhamer, J.
Scheiblhofer, S.
Balic, N.
Horak, F.
Ollert, M.
Papadopoulos, N. G.
Quirce, S.
Szepfalusi, Z.
Herz, U.
van Tol, E. A. F.
Spitzauer, S.
Valenta, R.
Infant milk formulas differ regarding their allergenic activity and induction of T‐cell and cytokine responses
title Infant milk formulas differ regarding their allergenic activity and induction of T‐cell and cytokine responses
title_full Infant milk formulas differ regarding their allergenic activity and induction of T‐cell and cytokine responses
title_fullStr Infant milk formulas differ regarding their allergenic activity and induction of T‐cell and cytokine responses
title_full_unstemmed Infant milk formulas differ regarding their allergenic activity and induction of T‐cell and cytokine responses
title_short Infant milk formulas differ regarding their allergenic activity and induction of T‐cell and cytokine responses
title_sort infant milk formulas differ regarding their allergenic activity and induction of t‐cell and cytokine responses
topic ORIGINAL ARTICLES
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5321598/
https://www.ncbi.nlm.nih.gov/pubmed/27455132
http://dx.doi.org/10.1111/all.12992
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