Cargando…
Layer-specific optogenetic activation of pyramidal neurons causes beta–gamma entrainment of neonatal networks
Coordinated activity patterns in the developing brain may contribute to the wiring of neuronal circuits underlying future behavioural requirements. However, causal evidence for this hypothesis has been difficult to obtain owing to the absence of tools for selective manipulation of oscillations durin...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5321724/ https://www.ncbi.nlm.nih.gov/pubmed/28216627 http://dx.doi.org/10.1038/ncomms14563 |
_version_ | 1782509726614945792 |
---|---|
author | Bitzenhofer, Sebastian H Ahlbeck, Joachim Wolff, Amy Wiegert, J. Simon Gee, Christine E. Oertner, Thomas G. Hanganu-Opatz, Ileana L. |
author_facet | Bitzenhofer, Sebastian H Ahlbeck, Joachim Wolff, Amy Wiegert, J. Simon Gee, Christine E. Oertner, Thomas G. Hanganu-Opatz, Ileana L. |
author_sort | Bitzenhofer, Sebastian H |
collection | PubMed |
description | Coordinated activity patterns in the developing brain may contribute to the wiring of neuronal circuits underlying future behavioural requirements. However, causal evidence for this hypothesis has been difficult to obtain owing to the absence of tools for selective manipulation of oscillations during early development. We established a protocol that combines optogenetics with electrophysiological recordings from neonatal mice in vivo to elucidate the substrate of early network oscillations in the prefrontal cortex. We show that light-induced activation of layer II/III pyramidal neurons that are transfected by in utero electroporation with a high-efficiency channelrhodopsin drives frequency-specific spiking and boosts network oscillations within beta–gamma frequency range. By contrast, activation of layer V/VI pyramidal neurons causes nonspecific network activation. Thus, entrainment of neonatal prefrontal networks in fast rhythms relies on the activation of layer II/III pyramidal neurons. This approach used here may be useful for further interrogation of developing circuits, and their behavioural readout. |
format | Online Article Text |
id | pubmed-5321724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53217242017-03-01 Layer-specific optogenetic activation of pyramidal neurons causes beta–gamma entrainment of neonatal networks Bitzenhofer, Sebastian H Ahlbeck, Joachim Wolff, Amy Wiegert, J. Simon Gee, Christine E. Oertner, Thomas G. Hanganu-Opatz, Ileana L. Nat Commun Article Coordinated activity patterns in the developing brain may contribute to the wiring of neuronal circuits underlying future behavioural requirements. However, causal evidence for this hypothesis has been difficult to obtain owing to the absence of tools for selective manipulation of oscillations during early development. We established a protocol that combines optogenetics with electrophysiological recordings from neonatal mice in vivo to elucidate the substrate of early network oscillations in the prefrontal cortex. We show that light-induced activation of layer II/III pyramidal neurons that are transfected by in utero electroporation with a high-efficiency channelrhodopsin drives frequency-specific spiking and boosts network oscillations within beta–gamma frequency range. By contrast, activation of layer V/VI pyramidal neurons causes nonspecific network activation. Thus, entrainment of neonatal prefrontal networks in fast rhythms relies on the activation of layer II/III pyramidal neurons. This approach used here may be useful for further interrogation of developing circuits, and their behavioural readout. Nature Publishing Group 2017-02-20 /pmc/articles/PMC5321724/ /pubmed/28216627 http://dx.doi.org/10.1038/ncomms14563 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Bitzenhofer, Sebastian H Ahlbeck, Joachim Wolff, Amy Wiegert, J. Simon Gee, Christine E. Oertner, Thomas G. Hanganu-Opatz, Ileana L. Layer-specific optogenetic activation of pyramidal neurons causes beta–gamma entrainment of neonatal networks |
title | Layer-specific optogenetic activation of pyramidal neurons causes beta–gamma entrainment of neonatal networks |
title_full | Layer-specific optogenetic activation of pyramidal neurons causes beta–gamma entrainment of neonatal networks |
title_fullStr | Layer-specific optogenetic activation of pyramidal neurons causes beta–gamma entrainment of neonatal networks |
title_full_unstemmed | Layer-specific optogenetic activation of pyramidal neurons causes beta–gamma entrainment of neonatal networks |
title_short | Layer-specific optogenetic activation of pyramidal neurons causes beta–gamma entrainment of neonatal networks |
title_sort | layer-specific optogenetic activation of pyramidal neurons causes beta–gamma entrainment of neonatal networks |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5321724/ https://www.ncbi.nlm.nih.gov/pubmed/28216627 http://dx.doi.org/10.1038/ncomms14563 |
work_keys_str_mv | AT bitzenhofersebastianh layerspecificoptogeneticactivationofpyramidalneuronscausesbetagammaentrainmentofneonatalnetworks AT ahlbeckjoachim layerspecificoptogeneticactivationofpyramidalneuronscausesbetagammaentrainmentofneonatalnetworks AT wolffamy layerspecificoptogeneticactivationofpyramidalneuronscausesbetagammaentrainmentofneonatalnetworks AT wiegertjsimon layerspecificoptogeneticactivationofpyramidalneuronscausesbetagammaentrainmentofneonatalnetworks AT geechristinee layerspecificoptogeneticactivationofpyramidalneuronscausesbetagammaentrainmentofneonatalnetworks AT oertnerthomasg layerspecificoptogeneticactivationofpyramidalneuronscausesbetagammaentrainmentofneonatalnetworks AT hanganuopatzileanal layerspecificoptogeneticactivationofpyramidalneuronscausesbetagammaentrainmentofneonatalnetworks |