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Ectopic expression of Arabidopsis Target of Rapamycin (AtTOR) improves water-use efficiency and yield potential in rice

The target of Rapamycin (TOR) present in all eukaryotes is a multifunctional protein, regulating growth, development, protein translation, ribosome biogenesis, nutrient, and energy signaling. In the present study, ectopic expression of TOR gene of Arabidopsis thaliana in a widely cultivated indica r...

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Autores principales: Bakshi, Achala, Moin, Mazahar, Kumar, M. Udaya, Reddy, Aramati Bindu Madhava, Ren, Maozhi, Datla, Raju, Siddiq, E. A., Kirti, P. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322334/
https://www.ncbi.nlm.nih.gov/pubmed/28230163
http://dx.doi.org/10.1038/srep42835
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author Bakshi, Achala
Moin, Mazahar
Kumar, M. Udaya
Reddy, Aramati Bindu Madhava
Ren, Maozhi
Datla, Raju
Siddiq, E. A.
Kirti, P. B.
author_facet Bakshi, Achala
Moin, Mazahar
Kumar, M. Udaya
Reddy, Aramati Bindu Madhava
Ren, Maozhi
Datla, Raju
Siddiq, E. A.
Kirti, P. B.
author_sort Bakshi, Achala
collection PubMed
description The target of Rapamycin (TOR) present in all eukaryotes is a multifunctional protein, regulating growth, development, protein translation, ribosome biogenesis, nutrient, and energy signaling. In the present study, ectopic expression of TOR gene of Arabidopsis thaliana in a widely cultivated indica rice resulted in enhanced plant growth under water-limiting conditions conferring agronomically important water-use efficiency (WUE) trait. The AtTOR high expression lines of rice exhibited profuse tillering, increased panicle length, increased plant height, high photosynthetic efficiency, chlorophyll content and low ∆(13)C. Δ(13)C, which is inversely related to high WUE, was as low as 17‰ in two AtTOR high expression lines. These lines were also insensitive to the ABA-mediated inhibition of seed germination. The significant upregulation of 15 stress-specific genes in high expression lines indicates their contribution to abiotic stress tolerance. The constitutive expression of AtTOR is also associated with significant transcriptional upregulation of putative TOR complex-1 components, OsRaptor and OsLST8. Glucose-mediated transcriptional activation of AtTOR gene enhanced lateral root formation. Taken together, our findings indicate that TOR, in addition to its multiple cellular functions, also plays an important role in response to abiotic stress and potentially enhances WUE and yield related attributes.
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spelling pubmed-53223342017-03-01 Ectopic expression of Arabidopsis Target of Rapamycin (AtTOR) improves water-use efficiency and yield potential in rice Bakshi, Achala Moin, Mazahar Kumar, M. Udaya Reddy, Aramati Bindu Madhava Ren, Maozhi Datla, Raju Siddiq, E. A. Kirti, P. B. Sci Rep Article The target of Rapamycin (TOR) present in all eukaryotes is a multifunctional protein, regulating growth, development, protein translation, ribosome biogenesis, nutrient, and energy signaling. In the present study, ectopic expression of TOR gene of Arabidopsis thaliana in a widely cultivated indica rice resulted in enhanced plant growth under water-limiting conditions conferring agronomically important water-use efficiency (WUE) trait. The AtTOR high expression lines of rice exhibited profuse tillering, increased panicle length, increased plant height, high photosynthetic efficiency, chlorophyll content and low ∆(13)C. Δ(13)C, which is inversely related to high WUE, was as low as 17‰ in two AtTOR high expression lines. These lines were also insensitive to the ABA-mediated inhibition of seed germination. The significant upregulation of 15 stress-specific genes in high expression lines indicates their contribution to abiotic stress tolerance. The constitutive expression of AtTOR is also associated with significant transcriptional upregulation of putative TOR complex-1 components, OsRaptor and OsLST8. Glucose-mediated transcriptional activation of AtTOR gene enhanced lateral root formation. Taken together, our findings indicate that TOR, in addition to its multiple cellular functions, also plays an important role in response to abiotic stress and potentially enhances WUE and yield related attributes. Nature Publishing Group 2017-02-23 /pmc/articles/PMC5322334/ /pubmed/28230163 http://dx.doi.org/10.1038/srep42835 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Bakshi, Achala
Moin, Mazahar
Kumar, M. Udaya
Reddy, Aramati Bindu Madhava
Ren, Maozhi
Datla, Raju
Siddiq, E. A.
Kirti, P. B.
Ectopic expression of Arabidopsis Target of Rapamycin (AtTOR) improves water-use efficiency and yield potential in rice
title Ectopic expression of Arabidopsis Target of Rapamycin (AtTOR) improves water-use efficiency and yield potential in rice
title_full Ectopic expression of Arabidopsis Target of Rapamycin (AtTOR) improves water-use efficiency and yield potential in rice
title_fullStr Ectopic expression of Arabidopsis Target of Rapamycin (AtTOR) improves water-use efficiency and yield potential in rice
title_full_unstemmed Ectopic expression of Arabidopsis Target of Rapamycin (AtTOR) improves water-use efficiency and yield potential in rice
title_short Ectopic expression of Arabidopsis Target of Rapamycin (AtTOR) improves water-use efficiency and yield potential in rice
title_sort ectopic expression of arabidopsis target of rapamycin (attor) improves water-use efficiency and yield potential in rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322334/
https://www.ncbi.nlm.nih.gov/pubmed/28230163
http://dx.doi.org/10.1038/srep42835
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