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Highly sensitive and adaptable fluorescence-quenched pair discloses the substrate specificity profiles in diverse protease families

Internally quenched fluorescent (IQF) peptide substrates originating from FRET (Förster Resonance Energy Transfer) are powerful tool for examining the activity and specificity of proteases, and a variety of donor/acceptor pairs are extensively used to design individual substrates and combinatorial l...

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Detalles Bibliográficos
Autores principales: Poreba, Marcin, Szalek, Aleksandra, Rut, Wioletta, Kasperkiewicz, Paulina, Rutkowska-Wlodarczyk, Izabela, Snipas, Scott J., Itoh, Yoshifumi, Turk, Dusan, Turk, Boris, Overall, Christopher M., Kaczmarek, Leszek, Salvesen, Guy S., Drag, Marcin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322338/
https://www.ncbi.nlm.nih.gov/pubmed/28230157
http://dx.doi.org/10.1038/srep43135

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