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Genetic diversity, transmission dynamics and drug resistance of Mycobacterium tuberculosis in Angola
Tuberculosis (TB) poses a serious public health problem in Angola. No surveillance data on drug resistance is available and nothing is known regarding the genetic diversity and population structure of circulating Mycobacterium tuberculosis strains. Here, we have genotyped and evaluated drug suscepti...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322374/ https://www.ncbi.nlm.nih.gov/pubmed/28230095 http://dx.doi.org/10.1038/srep42814 |
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author | Perdigão, João Clemente, Sofia Ramos, Jorge Masakidi, Pedro Machado, Diana Silva, Carla Couto, Isabel Viveiros, Miguel Taveira, Nuno Portugal, Isabel |
author_facet | Perdigão, João Clemente, Sofia Ramos, Jorge Masakidi, Pedro Machado, Diana Silva, Carla Couto, Isabel Viveiros, Miguel Taveira, Nuno Portugal, Isabel |
author_sort | Perdigão, João |
collection | PubMed |
description | Tuberculosis (TB) poses a serious public health problem in Angola. No surveillance data on drug resistance is available and nothing is known regarding the genetic diversity and population structure of circulating Mycobacterium tuberculosis strains. Here, we have genotyped and evaluated drug susceptibility of 89 Mycobacterium tuberculosis clinical isolates from Luanda. Thirty-three different spoligotype profiles corresponding to 24 different Shared International Types (SIT) and 9 orphan profiles were detected. SIT 20 (LAM1) was the most prevalent (n = 16, 18.2%) followed by SIT 42 (LAM9; n = 15, 17.1%). Overall, the M. tuberculosis population structure in this sample was dominated by LAM (64.8%) and T (33.0%) strains. Twenty-four-loci MIRU-VNTR analysis revealed that a total of 13 isolates were grouped in 5 distinct clusters. Drug susceptibility data showed that 22 (24.7%) of the 89 clinical isolates were resistant to one or more antibacillary drugs of which 4 (4.5%) were multidrug resistant. In conclusion, this study demonstrates a high predominance of LAM strains circulating in the Luanda setting and the presence of recent transmission events. The rate and the emergence dynamics of drug resistant TB found in this sample are significant and highlight the need of further studies specifically focused on MDR-TB transmission. |
format | Online Article Text |
id | pubmed-5322374 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-53223742017-03-01 Genetic diversity, transmission dynamics and drug resistance of Mycobacterium tuberculosis in Angola Perdigão, João Clemente, Sofia Ramos, Jorge Masakidi, Pedro Machado, Diana Silva, Carla Couto, Isabel Viveiros, Miguel Taveira, Nuno Portugal, Isabel Sci Rep Article Tuberculosis (TB) poses a serious public health problem in Angola. No surveillance data on drug resistance is available and nothing is known regarding the genetic diversity and population structure of circulating Mycobacterium tuberculosis strains. Here, we have genotyped and evaluated drug susceptibility of 89 Mycobacterium tuberculosis clinical isolates from Luanda. Thirty-three different spoligotype profiles corresponding to 24 different Shared International Types (SIT) and 9 orphan profiles were detected. SIT 20 (LAM1) was the most prevalent (n = 16, 18.2%) followed by SIT 42 (LAM9; n = 15, 17.1%). Overall, the M. tuberculosis population structure in this sample was dominated by LAM (64.8%) and T (33.0%) strains. Twenty-four-loci MIRU-VNTR analysis revealed that a total of 13 isolates were grouped in 5 distinct clusters. Drug susceptibility data showed that 22 (24.7%) of the 89 clinical isolates were resistant to one or more antibacillary drugs of which 4 (4.5%) were multidrug resistant. In conclusion, this study demonstrates a high predominance of LAM strains circulating in the Luanda setting and the presence of recent transmission events. The rate and the emergence dynamics of drug resistant TB found in this sample are significant and highlight the need of further studies specifically focused on MDR-TB transmission. Nature Publishing Group 2017-02-23 /pmc/articles/PMC5322374/ /pubmed/28230095 http://dx.doi.org/10.1038/srep42814 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Perdigão, João Clemente, Sofia Ramos, Jorge Masakidi, Pedro Machado, Diana Silva, Carla Couto, Isabel Viveiros, Miguel Taveira, Nuno Portugal, Isabel Genetic diversity, transmission dynamics and drug resistance of Mycobacterium tuberculosis in Angola |
title | Genetic diversity, transmission dynamics and drug resistance of Mycobacterium tuberculosis in Angola |
title_full | Genetic diversity, transmission dynamics and drug resistance of Mycobacterium tuberculosis in Angola |
title_fullStr | Genetic diversity, transmission dynamics and drug resistance of Mycobacterium tuberculosis in Angola |
title_full_unstemmed | Genetic diversity, transmission dynamics and drug resistance of Mycobacterium tuberculosis in Angola |
title_short | Genetic diversity, transmission dynamics and drug resistance of Mycobacterium tuberculosis in Angola |
title_sort | genetic diversity, transmission dynamics and drug resistance of mycobacterium tuberculosis in angola |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322374/ https://www.ncbi.nlm.nih.gov/pubmed/28230095 http://dx.doi.org/10.1038/srep42814 |
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