Cargando…

Cytoplasmic deadenylase Ccr4 is required for translational repression of LRG1 mRNA in the stationary phase

Ccr4 is a major cytoplasmic deadenylase involved in mRNA poly(A) tail shortening in Saccharomyces cerevisiae. We have previously shown that Ccr4 negatively regulates expression of LRG1 mRNA encoding a GTPase-activating protein for the small GTPase Rho1, a component of cell wall integrity pathway, an...

Descripción completa

Detalles Bibliográficos
Autores principales: Duy, Duong Long, Suda, Yasuyuki, Irie, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322899/
https://www.ncbi.nlm.nih.gov/pubmed/28231297
http://dx.doi.org/10.1371/journal.pone.0172476
_version_ 1782509933265158144
author Duy, Duong Long
Suda, Yasuyuki
Irie, Kenji
author_facet Duy, Duong Long
Suda, Yasuyuki
Irie, Kenji
author_sort Duy, Duong Long
collection PubMed
description Ccr4 is a major cytoplasmic deadenylase involved in mRNA poly(A) tail shortening in Saccharomyces cerevisiae. We have previously shown that Ccr4 negatively regulates expression of LRG1 mRNA encoding a GTPase-activating protein for the small GTPase Rho1, a component of cell wall integrity pathway, and deletion of LRG1 suppresses the temperature-sensitive growth defect of the ccr4Δ mutant. We have also shown that the slow growth of the ccr4Δ mutant is suppressed by deletion of another gene, PBP1, encoding a poly(A)-binding protein (Pab1)-binding protein 1; however, the underlying mechanism still remains unknown. In this study, we investigated how ccr4Δ and pbp1Δ mutations influence on the length of poly(A) tail and LRG1 mRNA and protein levels during long-term cultivation. In the log-phase ccr4Δ mutant cells, LRG1 poly(A) tail was longer and LRG1 mRNA level was higher than those in the log-phase wild-type (WT) cells. Unexpectedly, Lrg1 protein level in the ccr4Δ mutant cells was comparable with that in WT. In the stationary-phase ccr4Δ mutant cells, LRG1 poly(A) tail length was still longer and LRG1 mRNA level was still higher than those in WT cells. In contrast to the log phase, Lrg1 protein level in the stationary-phase ccr4Δ mutant cells was maintained much higher than that in the stationary-phase WT cells. Consistently, active translating ribosomes still remained abundant in the stationary-phase ccr4Δ mutant cells, whereas they were strongly decreased in the stationary-phase WT cells. Loss of PBP1 reduced the LRG1 poly(A) tail length as well as LRG1 mRNA and protein levels in the stationary-phase ccr4Δ mutant cells. Our results suggest that Ccr4 regulates not only LRG1 mRNA level through poly(A) shortening but also the translation of LRG1 mRNA, and that Pbp1 is involved in the Ccr4-mediated regulation of mRNA stability and translation.
format Online
Article
Text
id pubmed-5322899
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-53228992017-03-09 Cytoplasmic deadenylase Ccr4 is required for translational repression of LRG1 mRNA in the stationary phase Duy, Duong Long Suda, Yasuyuki Irie, Kenji PLoS One Research Article Ccr4 is a major cytoplasmic deadenylase involved in mRNA poly(A) tail shortening in Saccharomyces cerevisiae. We have previously shown that Ccr4 negatively regulates expression of LRG1 mRNA encoding a GTPase-activating protein for the small GTPase Rho1, a component of cell wall integrity pathway, and deletion of LRG1 suppresses the temperature-sensitive growth defect of the ccr4Δ mutant. We have also shown that the slow growth of the ccr4Δ mutant is suppressed by deletion of another gene, PBP1, encoding a poly(A)-binding protein (Pab1)-binding protein 1; however, the underlying mechanism still remains unknown. In this study, we investigated how ccr4Δ and pbp1Δ mutations influence on the length of poly(A) tail and LRG1 mRNA and protein levels during long-term cultivation. In the log-phase ccr4Δ mutant cells, LRG1 poly(A) tail was longer and LRG1 mRNA level was higher than those in the log-phase wild-type (WT) cells. Unexpectedly, Lrg1 protein level in the ccr4Δ mutant cells was comparable with that in WT. In the stationary-phase ccr4Δ mutant cells, LRG1 poly(A) tail length was still longer and LRG1 mRNA level was still higher than those in WT cells. In contrast to the log phase, Lrg1 protein level in the stationary-phase ccr4Δ mutant cells was maintained much higher than that in the stationary-phase WT cells. Consistently, active translating ribosomes still remained abundant in the stationary-phase ccr4Δ mutant cells, whereas they were strongly decreased in the stationary-phase WT cells. Loss of PBP1 reduced the LRG1 poly(A) tail length as well as LRG1 mRNA and protein levels in the stationary-phase ccr4Δ mutant cells. Our results suggest that Ccr4 regulates not only LRG1 mRNA level through poly(A) shortening but also the translation of LRG1 mRNA, and that Pbp1 is involved in the Ccr4-mediated regulation of mRNA stability and translation. Public Library of Science 2017-02-23 /pmc/articles/PMC5322899/ /pubmed/28231297 http://dx.doi.org/10.1371/journal.pone.0172476 Text en © 2017 Duy et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Duy, Duong Long
Suda, Yasuyuki
Irie, Kenji
Cytoplasmic deadenylase Ccr4 is required for translational repression of LRG1 mRNA in the stationary phase
title Cytoplasmic deadenylase Ccr4 is required for translational repression of LRG1 mRNA in the stationary phase
title_full Cytoplasmic deadenylase Ccr4 is required for translational repression of LRG1 mRNA in the stationary phase
title_fullStr Cytoplasmic deadenylase Ccr4 is required for translational repression of LRG1 mRNA in the stationary phase
title_full_unstemmed Cytoplasmic deadenylase Ccr4 is required for translational repression of LRG1 mRNA in the stationary phase
title_short Cytoplasmic deadenylase Ccr4 is required for translational repression of LRG1 mRNA in the stationary phase
title_sort cytoplasmic deadenylase ccr4 is required for translational repression of lrg1 mrna in the stationary phase
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322899/
https://www.ncbi.nlm.nih.gov/pubmed/28231297
http://dx.doi.org/10.1371/journal.pone.0172476
work_keys_str_mv AT duyduonglong cytoplasmicdeadenylaseccr4isrequiredfortranslationalrepressionoflrg1mrnainthestationaryphase
AT sudayasuyuki cytoplasmicdeadenylaseccr4isrequiredfortranslationalrepressionoflrg1mrnainthestationaryphase
AT iriekenji cytoplasmicdeadenylaseccr4isrequiredfortranslationalrepressionoflrg1mrnainthestationaryphase