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Development of a keratinase activity assay using recombinant chicken feather keratin substrates
Poultry feathers consist mainly of the protein keratin, which is rich in β-pleated sheets and consequently resistant to proteolysis. Although many keratinases have been identified, the reasons for their substrate specificity towards β-keratin remain unclear due to difficulties in preparing a soluble...
Autores principales: | Jin, Hyeon-Su, Park, Seon Yeong, Kim, Kyungmin, Lee, Yong-Jik, Nam, Gae-Won, Kang, Nam Joo, Lee, Dong-Woo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322917/ https://www.ncbi.nlm.nih.gov/pubmed/28231319 http://dx.doi.org/10.1371/journal.pone.0172712 |
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