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The pathogens profile in children with otitis media with effusion and adenoid hypertrophy

OBJECTIVES: To evaluate the presence of viruses and bacteria in middle ear and adenoids of patients with and without otitis media with effusion (OME). METHODS: Adenoid samples and middle ear washes (MEW) were obtained from children with OME associated with adenoid hypertrophy undergoing adenoidectom...

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Autores principales: Buzatto, G. P., Tamashiro, E., Proenca-Modena, J. L., Saturno, T. H., Prates, M. C., Gagliardi, T. B., Carenzi, L. R., Massuda, E. T., Hyppolito, M. A., Valera, F. C. P., Arruda, E., Anselmo-Lima, W. T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322954/
https://www.ncbi.nlm.nih.gov/pubmed/28231295
http://dx.doi.org/10.1371/journal.pone.0171049
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author Buzatto, G. P.
Tamashiro, E.
Proenca-Modena, J. L.
Saturno, T. H.
Prates, M. C.
Gagliardi, T. B.
Carenzi, L. R.
Massuda, E. T.
Hyppolito, M. A.
Valera, F. C. P.
Arruda, E.
Anselmo-Lima, W. T.
author_facet Buzatto, G. P.
Tamashiro, E.
Proenca-Modena, J. L.
Saturno, T. H.
Prates, M. C.
Gagliardi, T. B.
Carenzi, L. R.
Massuda, E. T.
Hyppolito, M. A.
Valera, F. C. P.
Arruda, E.
Anselmo-Lima, W. T.
author_sort Buzatto, G. P.
collection PubMed
description OBJECTIVES: To evaluate the presence of viruses and bacteria in middle ear and adenoids of patients with and without otitis media with effusion (OME). METHODS: Adenoid samples and middle ear washes (MEW) were obtained from children with OME associated with adenoid hypertrophy undergoing adenoidectomy and tympanostomy, and compared to those obtained from patients undergoing cochlear implant surgery, as a control group. Specific DNA or RNA of 9 respiratory viruses (rhinovirus, influenza virus, picornavirus, syncytial respiratory virus, metapneumovirus, coronavirus, enterovirus, adenovirus and bocavirus) and 5 bacteria (S. pneumoniae, H. influenzae, M. catarrhalis, P. aeruginosa and S. aureus) were extracted and quantified by real-time PCR. RESULTS: 37 OME and 14 cochlear implant children were included in the study. At the adenoid, virus and bacteria were similarly detected in both OME and control patients. At the middle ear washes, however, a higher prevalence of bacteria was observed in patients with OME (p = 0.01). S. pneumoniae (p = 0.01) and M. catarrhalis (p = 0.022) were the bacteria responsible for this difference. Although total virus detection was not statistically different from controls at the middle ear washes (p = 0.065), adenovirus was detected in higher proportions in adenoid samples of OME patients than controls (p = 0.019). CONCLUSIONS: Despite both OME and control patients presented similar rates of viruses and bacteria at the adenoid, children with OME presented higher prevalence of S. pneumonia, M. catarrhalis in middle ear and adenovirus in adenoids when compared to controls. These findings could suggest that these pathogens could contribute to the fluid persistence in the middle ear.
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spelling pubmed-53229542017-03-09 The pathogens profile in children with otitis media with effusion and adenoid hypertrophy Buzatto, G. P. Tamashiro, E. Proenca-Modena, J. L. Saturno, T. H. Prates, M. C. Gagliardi, T. B. Carenzi, L. R. Massuda, E. T. Hyppolito, M. A. Valera, F. C. P. Arruda, E. Anselmo-Lima, W. T. PLoS One Research Article OBJECTIVES: To evaluate the presence of viruses and bacteria in middle ear and adenoids of patients with and without otitis media with effusion (OME). METHODS: Adenoid samples and middle ear washes (MEW) were obtained from children with OME associated with adenoid hypertrophy undergoing adenoidectomy and tympanostomy, and compared to those obtained from patients undergoing cochlear implant surgery, as a control group. Specific DNA or RNA of 9 respiratory viruses (rhinovirus, influenza virus, picornavirus, syncytial respiratory virus, metapneumovirus, coronavirus, enterovirus, adenovirus and bocavirus) and 5 bacteria (S. pneumoniae, H. influenzae, M. catarrhalis, P. aeruginosa and S. aureus) were extracted and quantified by real-time PCR. RESULTS: 37 OME and 14 cochlear implant children were included in the study. At the adenoid, virus and bacteria were similarly detected in both OME and control patients. At the middle ear washes, however, a higher prevalence of bacteria was observed in patients with OME (p = 0.01). S. pneumoniae (p = 0.01) and M. catarrhalis (p = 0.022) were the bacteria responsible for this difference. Although total virus detection was not statistically different from controls at the middle ear washes (p = 0.065), adenovirus was detected in higher proportions in adenoid samples of OME patients than controls (p = 0.019). CONCLUSIONS: Despite both OME and control patients presented similar rates of viruses and bacteria at the adenoid, children with OME presented higher prevalence of S. pneumonia, M. catarrhalis in middle ear and adenovirus in adenoids when compared to controls. These findings could suggest that these pathogens could contribute to the fluid persistence in the middle ear. Public Library of Science 2017-02-23 /pmc/articles/PMC5322954/ /pubmed/28231295 http://dx.doi.org/10.1371/journal.pone.0171049 Text en © 2017 Buzatto et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Buzatto, G. P.
Tamashiro, E.
Proenca-Modena, J. L.
Saturno, T. H.
Prates, M. C.
Gagliardi, T. B.
Carenzi, L. R.
Massuda, E. T.
Hyppolito, M. A.
Valera, F. C. P.
Arruda, E.
Anselmo-Lima, W. T.
The pathogens profile in children with otitis media with effusion and adenoid hypertrophy
title The pathogens profile in children with otitis media with effusion and adenoid hypertrophy
title_full The pathogens profile in children with otitis media with effusion and adenoid hypertrophy
title_fullStr The pathogens profile in children with otitis media with effusion and adenoid hypertrophy
title_full_unstemmed The pathogens profile in children with otitis media with effusion and adenoid hypertrophy
title_short The pathogens profile in children with otitis media with effusion and adenoid hypertrophy
title_sort pathogens profile in children with otitis media with effusion and adenoid hypertrophy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5322954/
https://www.ncbi.nlm.nih.gov/pubmed/28231295
http://dx.doi.org/10.1371/journal.pone.0171049
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