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CD147 silencing inhibits tumor growth by suppressing glucose transport in melanoma
Melanoma is a very malignant disease and there are still no effective treatments. CD147 participates in the carcinogenesis of multiple human cancers and GLUT-1, as a glucose transporter, is associated with tumor growth. However, the function of CD147 and GLUT-1 in melanoma have not been completely u...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323115/ https://www.ncbi.nlm.nih.gov/pubmed/27556188 http://dx.doi.org/10.18632/oncotarget.11415 |
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author | Su, Juan Gao, Tianyuan Jiang, Minghao Wu, Lisha Zeng, Weiqi Zhao, Shuang Peng, Cong Chen, Xiang |
author_facet | Su, Juan Gao, Tianyuan Jiang, Minghao Wu, Lisha Zeng, Weiqi Zhao, Shuang Peng, Cong Chen, Xiang |
author_sort | Su, Juan |
collection | PubMed |
description | Melanoma is a very malignant disease and there are still no effective treatments. CD147 participates in the carcinogenesis of multiple human cancers and GLUT-1, as a glucose transporter, is associated with tumor growth. However, the function of CD147 and GLUT-1 in melanoma have not been completely understood. Thus, in this study we investigated the expression of CD147 and GLUT-1 in melanoma tissue, which were overexpressed compared with that in nevus tissue. In addition, CD147 and GLUT-1 were co-localized in the cytoplasm of human melanoma A375 cells. Immunoprecipitation proved that CD147 interacted with GLUT-1 at D105-199. Silencing CD147 by specific siRNA could downregulate GLUT-1 level via inhibiting PI3K/Akt signaling and decrease glucose uptake in A375 cells. In vivo experiments also supported that CD147 knockdown suppressed the tumor growth in melanoma subcutaneous mice model, observed by micro PET/CT. Our results could help validate CD147 as a new therapeutic target for treating melanoma. |
format | Online Article Text |
id | pubmed-5323115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53231152017-03-23 CD147 silencing inhibits tumor growth by suppressing glucose transport in melanoma Su, Juan Gao, Tianyuan Jiang, Minghao Wu, Lisha Zeng, Weiqi Zhao, Shuang Peng, Cong Chen, Xiang Oncotarget Research Paper Melanoma is a very malignant disease and there are still no effective treatments. CD147 participates in the carcinogenesis of multiple human cancers and GLUT-1, as a glucose transporter, is associated with tumor growth. However, the function of CD147 and GLUT-1 in melanoma have not been completely understood. Thus, in this study we investigated the expression of CD147 and GLUT-1 in melanoma tissue, which were overexpressed compared with that in nevus tissue. In addition, CD147 and GLUT-1 were co-localized in the cytoplasm of human melanoma A375 cells. Immunoprecipitation proved that CD147 interacted with GLUT-1 at D105-199. Silencing CD147 by specific siRNA could downregulate GLUT-1 level via inhibiting PI3K/Akt signaling and decrease glucose uptake in A375 cells. In vivo experiments also supported that CD147 knockdown suppressed the tumor growth in melanoma subcutaneous mice model, observed by micro PET/CT. Our results could help validate CD147 as a new therapeutic target for treating melanoma. Impact Journals LLC 2016-08-19 /pmc/articles/PMC5323115/ /pubmed/27556188 http://dx.doi.org/10.18632/oncotarget.11415 Text en Copyright: © 2016 Su et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Su, Juan Gao, Tianyuan Jiang, Minghao Wu, Lisha Zeng, Weiqi Zhao, Shuang Peng, Cong Chen, Xiang CD147 silencing inhibits tumor growth by suppressing glucose transport in melanoma |
title | CD147 silencing inhibits tumor growth by suppressing glucose transport in melanoma |
title_full | CD147 silencing inhibits tumor growth by suppressing glucose transport in melanoma |
title_fullStr | CD147 silencing inhibits tumor growth by suppressing glucose transport in melanoma |
title_full_unstemmed | CD147 silencing inhibits tumor growth by suppressing glucose transport in melanoma |
title_short | CD147 silencing inhibits tumor growth by suppressing glucose transport in melanoma |
title_sort | cd147 silencing inhibits tumor growth by suppressing glucose transport in melanoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323115/ https://www.ncbi.nlm.nih.gov/pubmed/27556188 http://dx.doi.org/10.18632/oncotarget.11415 |
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