Discovery of peptide inhibitors targeting human programmed death 1 (PD-1) receptor

Blocking the interaction of human programmed death 1 (hPD-1) and its ligand hPD-L1 has been a promising immunotherapy in cancer treatment. In this paper, using a computational de novo peptide design method, we designed several hPD-1 binding peptides. The most potent peptide Ar5Y_4 showed a KD value...

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Detalles Bibliográficos
Autores principales: Li, Qiao, Quan, Lina, Lyu, Jiankun, He, Zenghui, Wang, Xia, Meng, Jiajia, Zhao, Zhenjiang, Zhu, Lili, Liu, Xiaofeng, Li, Honglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323130/
https://www.ncbi.nlm.nih.gov/pubmed/27533458
http://dx.doi.org/10.18632/oncotarget.11274
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author Li, Qiao
Quan, Lina
Lyu, Jiankun
He, Zenghui
Wang, Xia
Meng, Jiajia
Zhao, Zhenjiang
Zhu, Lili
Liu, Xiaofeng
Li, Honglin
author_facet Li, Qiao
Quan, Lina
Lyu, Jiankun
He, Zenghui
Wang, Xia
Meng, Jiajia
Zhao, Zhenjiang
Zhu, Lili
Liu, Xiaofeng
Li, Honglin
author_sort Li, Qiao
collection PubMed
description Blocking the interaction of human programmed death 1 (hPD-1) and its ligand hPD-L1 has been a promising immunotherapy in cancer treatment. In this paper, using a computational de novo peptide design method, we designed several hPD-1 binding peptides. The most potent peptide Ar5Y_4 showed a KD value of 1.38 ± 0.39 μM, comparable to the binding affinity of the cognate hPD-L1. A Surface Plasmon Resonance (SPR) competitive binding assay result indicated that Ar5Y_4 could inhibit the interaction of hPD-1/hPD-L1. Moreover, Ar5Y_4 could restore the function of Jurkat T cells which had been suppressed by stimulated HCT116 cells. Peptides described in this paper provide promising biologic candidates for cancer immunotherapy or diagnostics.
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spelling pubmed-53231302017-03-23 Discovery of peptide inhibitors targeting human programmed death 1 (PD-1) receptor Li, Qiao Quan, Lina Lyu, Jiankun He, Zenghui Wang, Xia Meng, Jiajia Zhao, Zhenjiang Zhu, Lili Liu, Xiaofeng Li, Honglin Oncotarget Research Paper Blocking the interaction of human programmed death 1 (hPD-1) and its ligand hPD-L1 has been a promising immunotherapy in cancer treatment. In this paper, using a computational de novo peptide design method, we designed several hPD-1 binding peptides. The most potent peptide Ar5Y_4 showed a KD value of 1.38 ± 0.39 μM, comparable to the binding affinity of the cognate hPD-L1. A Surface Plasmon Resonance (SPR) competitive binding assay result indicated that Ar5Y_4 could inhibit the interaction of hPD-1/hPD-L1. Moreover, Ar5Y_4 could restore the function of Jurkat T cells which had been suppressed by stimulated HCT116 cells. Peptides described in this paper provide promising biologic candidates for cancer immunotherapy or diagnostics. Impact Journals LLC 2016-08-12 /pmc/articles/PMC5323130/ /pubmed/27533458 http://dx.doi.org/10.18632/oncotarget.11274 Text en Copyright: © 2016 Li et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Li, Qiao
Quan, Lina
Lyu, Jiankun
He, Zenghui
Wang, Xia
Meng, Jiajia
Zhao, Zhenjiang
Zhu, Lili
Liu, Xiaofeng
Li, Honglin
Discovery of peptide inhibitors targeting human programmed death 1 (PD-1) receptor
title Discovery of peptide inhibitors targeting human programmed death 1 (PD-1) receptor
title_full Discovery of peptide inhibitors targeting human programmed death 1 (PD-1) receptor
title_fullStr Discovery of peptide inhibitors targeting human programmed death 1 (PD-1) receptor
title_full_unstemmed Discovery of peptide inhibitors targeting human programmed death 1 (PD-1) receptor
title_short Discovery of peptide inhibitors targeting human programmed death 1 (PD-1) receptor
title_sort discovery of peptide inhibitors targeting human programmed death 1 (pd-1) receptor
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323130/
https://www.ncbi.nlm.nih.gov/pubmed/27533458
http://dx.doi.org/10.18632/oncotarget.11274
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