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Dux4 controls migration of mesenchymal stem cells through the Cxcr4-Sdf1 axis

We performed transcriptome profiling of human immortalized myoblasts (MB) transiently expressing double homeobox transcription factor 4 (DUX4) and double homeobox transcription factor 4 centromeric (DUX4c) and identified 114 and 70 genes differentially expressed in DUX4- and DUX4c-transfected myobla...

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Autores principales: Dmitriev, Petr, Kiseleva, Ekaterina, Kharchenko, Olga, Ivashkin, Evgeny, Pichugin, Andrei, Dessen, Philippe, Robert, Thomas, Coppée, Frédérique, Belayew, Alexandra, Carnac, Gilles, Laoudj-Chenivesse, Dalila, Lipinski, Marc, Vasiliev, Andrei, Vassetzky, Yegor S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323140/
https://www.ncbi.nlm.nih.gov/pubmed/27556182
http://dx.doi.org/10.18632/oncotarget.11368
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author Dmitriev, Petr
Kiseleva, Ekaterina
Kharchenko, Olga
Ivashkin, Evgeny
Pichugin, Andrei
Dessen, Philippe
Robert, Thomas
Coppée, Frédérique
Belayew, Alexandra
Carnac, Gilles
Laoudj-Chenivesse, Dalila
Lipinski, Marc
Vasiliev, Andrei
Vassetzky, Yegor S.
author_facet Dmitriev, Petr
Kiseleva, Ekaterina
Kharchenko, Olga
Ivashkin, Evgeny
Pichugin, Andrei
Dessen, Philippe
Robert, Thomas
Coppée, Frédérique
Belayew, Alexandra
Carnac, Gilles
Laoudj-Chenivesse, Dalila
Lipinski, Marc
Vasiliev, Andrei
Vassetzky, Yegor S.
author_sort Dmitriev, Petr
collection PubMed
description We performed transcriptome profiling of human immortalized myoblasts (MB) transiently expressing double homeobox transcription factor 4 (DUX4) and double homeobox transcription factor 4 centromeric (DUX4c) and identified 114 and 70 genes differentially expressed in DUX4- and DUX4c-transfected myoblasts, respectively. A significant number of differentially expressed genes were involved in inflammation, cellular migration and chemotaxis suggesting a role for DUX4 and DUX4c in these processes. DUX4 but not DUX4c overexpression resulted in upregulation of the CXCR4 (C-X-C motif Receptor 4) and CXCL12 (C-X-C motif ligand 12 also known as SDF1) expression in human immortalized myoblasts. In a Transwell cell migration assay, human bone marrow-derived mesenchymal stem cells (BMSCs) were migrating more efficiently towards human immortalized myoblasts overexpressing DUX4 as compared to controls; the migration efficiency of DUX4-transfected BMSCs was also increased. DUX4c overexpression in myoblasts or in BMSCs had no impact on the rate of BMSC migration. Antibodies against SDF1 and CXCR4 blocked the positive effect of DUX4 overexpression on BMSC migration. We propose that DUX4 controls the cellular migration of mesenchymal stem cells through the CXCR4 receptor.
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spelling pubmed-53231402017-03-23 Dux4 controls migration of mesenchymal stem cells through the Cxcr4-Sdf1 axis Dmitriev, Petr Kiseleva, Ekaterina Kharchenko, Olga Ivashkin, Evgeny Pichugin, Andrei Dessen, Philippe Robert, Thomas Coppée, Frédérique Belayew, Alexandra Carnac, Gilles Laoudj-Chenivesse, Dalila Lipinski, Marc Vasiliev, Andrei Vassetzky, Yegor S. Oncotarget Research Paper We performed transcriptome profiling of human immortalized myoblasts (MB) transiently expressing double homeobox transcription factor 4 (DUX4) and double homeobox transcription factor 4 centromeric (DUX4c) and identified 114 and 70 genes differentially expressed in DUX4- and DUX4c-transfected myoblasts, respectively. A significant number of differentially expressed genes were involved in inflammation, cellular migration and chemotaxis suggesting a role for DUX4 and DUX4c in these processes. DUX4 but not DUX4c overexpression resulted in upregulation of the CXCR4 (C-X-C motif Receptor 4) and CXCL12 (C-X-C motif ligand 12 also known as SDF1) expression in human immortalized myoblasts. In a Transwell cell migration assay, human bone marrow-derived mesenchymal stem cells (BMSCs) were migrating more efficiently towards human immortalized myoblasts overexpressing DUX4 as compared to controls; the migration efficiency of DUX4-transfected BMSCs was also increased. DUX4c overexpression in myoblasts or in BMSCs had no impact on the rate of BMSC migration. Antibodies against SDF1 and CXCR4 blocked the positive effect of DUX4 overexpression on BMSC migration. We propose that DUX4 controls the cellular migration of mesenchymal stem cells through the CXCR4 receptor. Impact Journals LLC 2016-08-18 /pmc/articles/PMC5323140/ /pubmed/27556182 http://dx.doi.org/10.18632/oncotarget.11368 Text en Copyright: © 2016 Dmitriev et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Dmitriev, Petr
Kiseleva, Ekaterina
Kharchenko, Olga
Ivashkin, Evgeny
Pichugin, Andrei
Dessen, Philippe
Robert, Thomas
Coppée, Frédérique
Belayew, Alexandra
Carnac, Gilles
Laoudj-Chenivesse, Dalila
Lipinski, Marc
Vasiliev, Andrei
Vassetzky, Yegor S.
Dux4 controls migration of mesenchymal stem cells through the Cxcr4-Sdf1 axis
title Dux4 controls migration of mesenchymal stem cells through the Cxcr4-Sdf1 axis
title_full Dux4 controls migration of mesenchymal stem cells through the Cxcr4-Sdf1 axis
title_fullStr Dux4 controls migration of mesenchymal stem cells through the Cxcr4-Sdf1 axis
title_full_unstemmed Dux4 controls migration of mesenchymal stem cells through the Cxcr4-Sdf1 axis
title_short Dux4 controls migration of mesenchymal stem cells through the Cxcr4-Sdf1 axis
title_sort dux4 controls migration of mesenchymal stem cells through the cxcr4-sdf1 axis
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323140/
https://www.ncbi.nlm.nih.gov/pubmed/27556182
http://dx.doi.org/10.18632/oncotarget.11368
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