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The small heat shock protein αA-crystallin negatively regulates pancreatic tumorigenesis
Our recent study has shown that αA-crystallin appears to act as a tumor suppressor in pancreas. Here, we analyzed expression patterns of αA-crystallin in the pancreatic tumor tissue and the neighbor normal tissue from 74 pancreatic cancer patients and also pancreatic cancer cell lines. Immunocytoche...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323194/ https://www.ncbi.nlm.nih.gov/pubmed/27588467 http://dx.doi.org/10.18632/oncotarget.11668 |
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author | Liu, Jifang Luo, Zhongwen Zhang, Lan Wang, Ling Nie, Qian Wang, Zheng-Feng Huang, Zhaoxia Hu, Xiaohui Gong, Lili Arrigo, Andre-Patrick Tang, Xiangcheng Xiang, Jia-Wen Liu, Fangyuan Deng, Mi Ji, Weike Hu, Wenfeng Zhu, Ji-Ye Chen, Baojiang Bridge, Julia Hollingsworth, Michael A. Gigantelli, James Liu, Yizhi Nguyen, Quan D. Li, David Wan-Cheng |
author_facet | Liu, Jifang Luo, Zhongwen Zhang, Lan Wang, Ling Nie, Qian Wang, Zheng-Feng Huang, Zhaoxia Hu, Xiaohui Gong, Lili Arrigo, Andre-Patrick Tang, Xiangcheng Xiang, Jia-Wen Liu, Fangyuan Deng, Mi Ji, Weike Hu, Wenfeng Zhu, Ji-Ye Chen, Baojiang Bridge, Julia Hollingsworth, Michael A. Gigantelli, James Liu, Yizhi Nguyen, Quan D. Li, David Wan-Cheng |
author_sort | Liu, Jifang |
collection | PubMed |
description | Our recent study has shown that αA-crystallin appears to act as a tumor suppressor in pancreas. Here, we analyzed expression patterns of αA-crystallin in the pancreatic tumor tissue and the neighbor normal tissue from 74 pancreatic cancer patients and also pancreatic cancer cell lines. Immunocytochemistry revealed that αA-crystallin was highly expressed in the normal tissue from 56 patients, but barely detectable in the pancreatic tumor tissue. Moreover, a low level of αA-crystallin predicts poor prognosis for patients with pancreatic duct adenocarcinoma (PDAC). In the 12 pancreatic cell lines analyzed, except for Capan-1 and Miapaca-2 where the level of αA-crystallin was about 80% and 65% of that in the control cell line, HPNE, the remaining pancreatic cancer cells have much lower αA-crystallin levels. Overexpression of αA-crystallin in MiaPaca-1 cells lacking endogenous αA-crystallin significantly decreased its tumorigenicity ability as shown in the colony formation and wound healing assays. In contrast, knockdown of αA-crystallin in the Capan-1 cells significantly increased its tumorigenicity ability as demonstrated in the above assays. Together, our results further demonstrate that αA-crystallin negatively regulates pancreatic tumorigenesis and appears to be a prognosis biomarker for PDAC. |
format | Online Article Text |
id | pubmed-5323194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53231942017-03-23 The small heat shock protein αA-crystallin negatively regulates pancreatic tumorigenesis Liu, Jifang Luo, Zhongwen Zhang, Lan Wang, Ling Nie, Qian Wang, Zheng-Feng Huang, Zhaoxia Hu, Xiaohui Gong, Lili Arrigo, Andre-Patrick Tang, Xiangcheng Xiang, Jia-Wen Liu, Fangyuan Deng, Mi Ji, Weike Hu, Wenfeng Zhu, Ji-Ye Chen, Baojiang Bridge, Julia Hollingsworth, Michael A. Gigantelli, James Liu, Yizhi Nguyen, Quan D. Li, David Wan-Cheng Oncotarget Research Paper Our recent study has shown that αA-crystallin appears to act as a tumor suppressor in pancreas. Here, we analyzed expression patterns of αA-crystallin in the pancreatic tumor tissue and the neighbor normal tissue from 74 pancreatic cancer patients and also pancreatic cancer cell lines. Immunocytochemistry revealed that αA-crystallin was highly expressed in the normal tissue from 56 patients, but barely detectable in the pancreatic tumor tissue. Moreover, a low level of αA-crystallin predicts poor prognosis for patients with pancreatic duct adenocarcinoma (PDAC). In the 12 pancreatic cell lines analyzed, except for Capan-1 and Miapaca-2 where the level of αA-crystallin was about 80% and 65% of that in the control cell line, HPNE, the remaining pancreatic cancer cells have much lower αA-crystallin levels. Overexpression of αA-crystallin in MiaPaca-1 cells lacking endogenous αA-crystallin significantly decreased its tumorigenicity ability as shown in the colony formation and wound healing assays. In contrast, knockdown of αA-crystallin in the Capan-1 cells significantly increased its tumorigenicity ability as demonstrated in the above assays. Together, our results further demonstrate that αA-crystallin negatively regulates pancreatic tumorigenesis and appears to be a prognosis biomarker for PDAC. Impact Journals LLC 2016-08-29 /pmc/articles/PMC5323194/ /pubmed/27588467 http://dx.doi.org/10.18632/oncotarget.11668 Text en Copyright: © 2016 Liu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Liu, Jifang Luo, Zhongwen Zhang, Lan Wang, Ling Nie, Qian Wang, Zheng-Feng Huang, Zhaoxia Hu, Xiaohui Gong, Lili Arrigo, Andre-Patrick Tang, Xiangcheng Xiang, Jia-Wen Liu, Fangyuan Deng, Mi Ji, Weike Hu, Wenfeng Zhu, Ji-Ye Chen, Baojiang Bridge, Julia Hollingsworth, Michael A. Gigantelli, James Liu, Yizhi Nguyen, Quan D. Li, David Wan-Cheng The small heat shock protein αA-crystallin negatively regulates pancreatic tumorigenesis |
title | The small heat shock protein αA-crystallin negatively regulates pancreatic tumorigenesis |
title_full | The small heat shock protein αA-crystallin negatively regulates pancreatic tumorigenesis |
title_fullStr | The small heat shock protein αA-crystallin negatively regulates pancreatic tumorigenesis |
title_full_unstemmed | The small heat shock protein αA-crystallin negatively regulates pancreatic tumorigenesis |
title_short | The small heat shock protein αA-crystallin negatively regulates pancreatic tumorigenesis |
title_sort | small heat shock protein αa-crystallin negatively regulates pancreatic tumorigenesis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323194/ https://www.ncbi.nlm.nih.gov/pubmed/27588467 http://dx.doi.org/10.18632/oncotarget.11668 |
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