Cargando…

c-Fos over-expression promotes radioresistance and predicts poor prognosis in malignant glioma

c-Fos is a major component of activator protein (AP)-1 complex. It has been implicated in cell differentiation, proliferation, angiogenesis, invasion, and metastasis. To investigate the role of c-Fos in glioma radiosensitivity and to understand the underlying molecular mechanisms, we downregulated c...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Zhi-Gang, Jiang, Guanmin, Tang, Jiao, Wang, Hui, Feng, Guokai, Chen, Furong, Tu, Ziwei, Liu, Guiyun, Zhao, Yu, Peng, Ming-Jing, He, Zheng-Wen, Chen, Xiao-Yan, Lindsay, Holly, Xia, Yun-Fei, Li, Xiao-Nan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323205/
https://www.ncbi.nlm.nih.gov/pubmed/27602752
http://dx.doi.org/10.18632/oncotarget.11779
_version_ 1782509990083297280
author Liu, Zhi-Gang
Jiang, Guanmin
Tang, Jiao
Wang, Hui
Feng, Guokai
Chen, Furong
Tu, Ziwei
Liu, Guiyun
Zhao, Yu
Peng, Ming-Jing
He, Zheng-Wen
Chen, Xiao-Yan
Lindsay, Holly
Xia, Yun-Fei
Li, Xiao-Nan
author_facet Liu, Zhi-Gang
Jiang, Guanmin
Tang, Jiao
Wang, Hui
Feng, Guokai
Chen, Furong
Tu, Ziwei
Liu, Guiyun
Zhao, Yu
Peng, Ming-Jing
He, Zheng-Wen
Chen, Xiao-Yan
Lindsay, Holly
Xia, Yun-Fei
Li, Xiao-Nan
author_sort Liu, Zhi-Gang
collection PubMed
description c-Fos is a major component of activator protein (AP)-1 complex. It has been implicated in cell differentiation, proliferation, angiogenesis, invasion, and metastasis. To investigate the role of c-Fos in glioma radiosensitivity and to understand the underlying molecular mechanisms, we downregulated c-Fos gene expression by lentivirus-mediated shRNA in glioma cell lines and subsequently analyzed the radiosensitivity, DNA damage repair capacity, and cell cycle distribution. Finally, we explored its prognostic value in 41 malignant glioma patients by immunohistochemistry. Our results showed that silencing c-Fos sensitized glioma cells to radiation by increasing radiation-induced DNA double strand breaks (DSBs), disturbing the DNA damage repair process, promoting G2/M cell cycle arrest, and enhancing apoptosis. c-Fos protein overexpression correlated with poor prognosis in malignant glioma patients treated with standard therapy. Our findings provide new insights into the mechanism of radioresistance in malignant glioma and identify c-Fos as a potentially novel therapeutic target for malignant glioma patients.
format Online
Article
Text
id pubmed-5323205
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-53232052017-03-23 c-Fos over-expression promotes radioresistance and predicts poor prognosis in malignant glioma Liu, Zhi-Gang Jiang, Guanmin Tang, Jiao Wang, Hui Feng, Guokai Chen, Furong Tu, Ziwei Liu, Guiyun Zhao, Yu Peng, Ming-Jing He, Zheng-Wen Chen, Xiao-Yan Lindsay, Holly Xia, Yun-Fei Li, Xiao-Nan Oncotarget Research Paper c-Fos is a major component of activator protein (AP)-1 complex. It has been implicated in cell differentiation, proliferation, angiogenesis, invasion, and metastasis. To investigate the role of c-Fos in glioma radiosensitivity and to understand the underlying molecular mechanisms, we downregulated c-Fos gene expression by lentivirus-mediated shRNA in glioma cell lines and subsequently analyzed the radiosensitivity, DNA damage repair capacity, and cell cycle distribution. Finally, we explored its prognostic value in 41 malignant glioma patients by immunohistochemistry. Our results showed that silencing c-Fos sensitized glioma cells to radiation by increasing radiation-induced DNA double strand breaks (DSBs), disturbing the DNA damage repair process, promoting G2/M cell cycle arrest, and enhancing apoptosis. c-Fos protein overexpression correlated with poor prognosis in malignant glioma patients treated with standard therapy. Our findings provide new insights into the mechanism of radioresistance in malignant glioma and identify c-Fos as a potentially novel therapeutic target for malignant glioma patients. Impact Journals LLC 2016-09-01 /pmc/articles/PMC5323205/ /pubmed/27602752 http://dx.doi.org/10.18632/oncotarget.11779 Text en Copyright: © 2016 Liu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Liu, Zhi-Gang
Jiang, Guanmin
Tang, Jiao
Wang, Hui
Feng, Guokai
Chen, Furong
Tu, Ziwei
Liu, Guiyun
Zhao, Yu
Peng, Ming-Jing
He, Zheng-Wen
Chen, Xiao-Yan
Lindsay, Holly
Xia, Yun-Fei
Li, Xiao-Nan
c-Fos over-expression promotes radioresistance and predicts poor prognosis in malignant glioma
title c-Fos over-expression promotes radioresistance and predicts poor prognosis in malignant glioma
title_full c-Fos over-expression promotes radioresistance and predicts poor prognosis in malignant glioma
title_fullStr c-Fos over-expression promotes radioresistance and predicts poor prognosis in malignant glioma
title_full_unstemmed c-Fos over-expression promotes radioresistance and predicts poor prognosis in malignant glioma
title_short c-Fos over-expression promotes radioresistance and predicts poor prognosis in malignant glioma
title_sort c-fos over-expression promotes radioresistance and predicts poor prognosis in malignant glioma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5323205/
https://www.ncbi.nlm.nih.gov/pubmed/27602752
http://dx.doi.org/10.18632/oncotarget.11779
work_keys_str_mv AT liuzhigang cfosoverexpressionpromotesradioresistanceandpredictspoorprognosisinmalignantglioma
AT jiangguanmin cfosoverexpressionpromotesradioresistanceandpredictspoorprognosisinmalignantglioma
AT tangjiao cfosoverexpressionpromotesradioresistanceandpredictspoorprognosisinmalignantglioma
AT wanghui cfosoverexpressionpromotesradioresistanceandpredictspoorprognosisinmalignantglioma
AT fengguokai cfosoverexpressionpromotesradioresistanceandpredictspoorprognosisinmalignantglioma
AT chenfurong cfosoverexpressionpromotesradioresistanceandpredictspoorprognosisinmalignantglioma
AT tuziwei cfosoverexpressionpromotesradioresistanceandpredictspoorprognosisinmalignantglioma
AT liuguiyun cfosoverexpressionpromotesradioresistanceandpredictspoorprognosisinmalignantglioma
AT zhaoyu cfosoverexpressionpromotesradioresistanceandpredictspoorprognosisinmalignantglioma
AT pengmingjing cfosoverexpressionpromotesradioresistanceandpredictspoorprognosisinmalignantglioma
AT hezhengwen cfosoverexpressionpromotesradioresistanceandpredictspoorprognosisinmalignantglioma
AT chenxiaoyan cfosoverexpressionpromotesradioresistanceandpredictspoorprognosisinmalignantglioma
AT lindsayholly cfosoverexpressionpromotesradioresistanceandpredictspoorprognosisinmalignantglioma
AT xiayunfei cfosoverexpressionpromotesradioresistanceandpredictspoorprognosisinmalignantglioma
AT lixiaonan cfosoverexpressionpromotesradioresistanceandpredictspoorprognosisinmalignantglioma