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Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti
BACKGROUND: Aedes aegypti is the main vector of important arboviruses such as dengue, Zika and chikungunya. During infections mosquitoes can activate the immune pathways Toll, IMD and JAK/STAT to limit pathogen replication. RESULTS: Here, we evaluate the immune response profile of Ae. aegypti agains...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5324288/ https://www.ncbi.nlm.nih.gov/pubmed/28231846 http://dx.doi.org/10.1186/s13071-017-2040-9 |
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author | Barletta, Ana Beatriz Ferreira Nascimento-Silva, Maria Clara L. Talyuli, Octávio A. C. Oliveira, José Henrique M. Pereira, Luiza Oliveira Ramos Oliveira, Pedro L. Sorgine, Marcos Henrique F. |
author_facet | Barletta, Ana Beatriz Ferreira Nascimento-Silva, Maria Clara L. Talyuli, Octávio A. C. Oliveira, José Henrique M. Pereira, Luiza Oliveira Ramos Oliveira, Pedro L. Sorgine, Marcos Henrique F. |
author_sort | Barletta, Ana Beatriz Ferreira |
collection | PubMed |
description | BACKGROUND: Aedes aegypti is the main vector of important arboviruses such as dengue, Zika and chikungunya. During infections mosquitoes can activate the immune pathways Toll, IMD and JAK/STAT to limit pathogen replication. RESULTS: Here, we evaluate the immune response profile of Ae. aegypti against Sindbis virus (SINV). We analyzed gene expression of components of Toll, IMD and JAK/STAT pathways and showed that a blood meal and virus infection upregulated aaREL2 in a microbiota-dependent fashion, since this induction was prevented by antibiotic. The presence of the microbiota activates IMD and impaired the replication of SINV in the midgut. Constitutive activation of the IMD pathway, by Caspar depletion, leads to a decrease in microbiota levels and an increase in SINV loads. CONCLUSION: Together, these results suggest that a blood meal is able to activate innate immune pathways, through a nutrient induced growth of microbiota, leading to upregulation of aaREL2 and IMD activation. Microbiota levels seemed to have a reciprocal interaction, where the proliferation of the microbiota activates IMD pathway that in turn controls bacterial levels, allowing SINV replication in Ae. aegypti mosquitoes. The activation of the IMD pathway seems to have an indirect effect in SINV levels that is induced by the microbiota. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-017-2040-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5324288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-53242882017-03-01 Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti Barletta, Ana Beatriz Ferreira Nascimento-Silva, Maria Clara L. Talyuli, Octávio A. C. Oliveira, José Henrique M. Pereira, Luiza Oliveira Ramos Oliveira, Pedro L. Sorgine, Marcos Henrique F. Parasit Vectors Research BACKGROUND: Aedes aegypti is the main vector of important arboviruses such as dengue, Zika and chikungunya. During infections mosquitoes can activate the immune pathways Toll, IMD and JAK/STAT to limit pathogen replication. RESULTS: Here, we evaluate the immune response profile of Ae. aegypti against Sindbis virus (SINV). We analyzed gene expression of components of Toll, IMD and JAK/STAT pathways and showed that a blood meal and virus infection upregulated aaREL2 in a microbiota-dependent fashion, since this induction was prevented by antibiotic. The presence of the microbiota activates IMD and impaired the replication of SINV in the midgut. Constitutive activation of the IMD pathway, by Caspar depletion, leads to a decrease in microbiota levels and an increase in SINV loads. CONCLUSION: Together, these results suggest that a blood meal is able to activate innate immune pathways, through a nutrient induced growth of microbiota, leading to upregulation of aaREL2 and IMD activation. Microbiota levels seemed to have a reciprocal interaction, where the proliferation of the microbiota activates IMD pathway that in turn controls bacterial levels, allowing SINV replication in Ae. aegypti mosquitoes. The activation of the IMD pathway seems to have an indirect effect in SINV levels that is induced by the microbiota. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-017-2040-9) contains supplementary material, which is available to authorized users. BioMed Central 2017-02-23 /pmc/articles/PMC5324288/ /pubmed/28231846 http://dx.doi.org/10.1186/s13071-017-2040-9 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Barletta, Ana Beatriz Ferreira Nascimento-Silva, Maria Clara L. Talyuli, Octávio A. C. Oliveira, José Henrique M. Pereira, Luiza Oliveira Ramos Oliveira, Pedro L. Sorgine, Marcos Henrique F. Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti |
title | Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti |
title_full | Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti |
title_fullStr | Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti |
title_full_unstemmed | Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti |
title_short | Microbiota activates IMD pathway and limits Sindbis infection in Aedes aegypti |
title_sort | microbiota activates imd pathway and limits sindbis infection in aedes aegypti |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5324288/ https://www.ncbi.nlm.nih.gov/pubmed/28231846 http://dx.doi.org/10.1186/s13071-017-2040-9 |
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