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NBP35 interacts with DRE2 in the maturation of cytosolic iron‐sulphur proteins in Arabidopsis thaliana
Proteins of the cytosolic pathway for iron‐sulphur (FeS) cluster assembly are conserved, except that plants lack a gene for CFD1 (Cytosolic FeS cluster Deficient 1). This poses the question of how NBP35 (Nucleotide‐Binding Protein 35 kDa), the heteromeric partner of CFD1 in metazoa, functions on its...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5324674/ https://www.ncbi.nlm.nih.gov/pubmed/27801963 http://dx.doi.org/10.1111/tpj.13409 |
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author | Bastow, Emma L. Bych, Katrine Crack, Jason C. Le Brun, Nick E. Balk, Janneke |
author_facet | Bastow, Emma L. Bych, Katrine Crack, Jason C. Le Brun, Nick E. Balk, Janneke |
author_sort | Bastow, Emma L. |
collection | PubMed |
description | Proteins of the cytosolic pathway for iron‐sulphur (FeS) cluster assembly are conserved, except that plants lack a gene for CFD1 (Cytosolic FeS cluster Deficient 1). This poses the question of how NBP35 (Nucleotide‐Binding Protein 35 kDa), the heteromeric partner of CFD1 in metazoa, functions on its own in plants. Firstly, we created viable mutant alleles of NBP35 in Arabidopsis to overcome embryo lethality of previously reported knockout mutations. RNAi knockdown lines with less than 30% NBP35 protein surprisingly showed no developmental or biochemical differences to wild‐type. Substitution of Cys14 to Ala, which destabilized the N‐terminal Fe(4)S(4) cluster in vitro, caused mild growth defects and a significant decrease in the activity of cytosolic FeS enzymes such as aconitase and aldehyde oxidases. The DNA glycosylase ROS1 was only partially decreased in activity and xanthine dehydrogenase not at all. Plants with strongly depleted NBP35 protein in combination with Cys14 to Ala substitution had distorted leaf development and decreased FeS enzyme activities. To find protein interaction partners of NBP35, a yeast‐two‐hybrid screen was carried out that identified NBP35 and DRE2 (Derepressed for Ribosomal protein S14 Expression). NBP35 is known to form a dimer, and DRE2 acts upstream in the cytosolic FeS protein assembly pathway. The NBP35–DRE2 interaction was not disrupted by Cys14 to Ala substitution. Our results show that NBP35 has a function in the maturation of FeS proteins that is conserved in plants, and is closely allied to the function of DRE2. |
format | Online Article Text |
id | pubmed-5324674 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53246742017-03-14 NBP35 interacts with DRE2 in the maturation of cytosolic iron‐sulphur proteins in Arabidopsis thaliana Bastow, Emma L. Bych, Katrine Crack, Jason C. Le Brun, Nick E. Balk, Janneke Plant J Original Articles Proteins of the cytosolic pathway for iron‐sulphur (FeS) cluster assembly are conserved, except that plants lack a gene for CFD1 (Cytosolic FeS cluster Deficient 1). This poses the question of how NBP35 (Nucleotide‐Binding Protein 35 kDa), the heteromeric partner of CFD1 in metazoa, functions on its own in plants. Firstly, we created viable mutant alleles of NBP35 in Arabidopsis to overcome embryo lethality of previously reported knockout mutations. RNAi knockdown lines with less than 30% NBP35 protein surprisingly showed no developmental or biochemical differences to wild‐type. Substitution of Cys14 to Ala, which destabilized the N‐terminal Fe(4)S(4) cluster in vitro, caused mild growth defects and a significant decrease in the activity of cytosolic FeS enzymes such as aconitase and aldehyde oxidases. The DNA glycosylase ROS1 was only partially decreased in activity and xanthine dehydrogenase not at all. Plants with strongly depleted NBP35 protein in combination with Cys14 to Ala substitution had distorted leaf development and decreased FeS enzyme activities. To find protein interaction partners of NBP35, a yeast‐two‐hybrid screen was carried out that identified NBP35 and DRE2 (Derepressed for Ribosomal protein S14 Expression). NBP35 is known to form a dimer, and DRE2 acts upstream in the cytosolic FeS protein assembly pathway. The NBP35–DRE2 interaction was not disrupted by Cys14 to Ala substitution. Our results show that NBP35 has a function in the maturation of FeS proteins that is conserved in plants, and is closely allied to the function of DRE2. John Wiley and Sons Inc. 2017-02-03 2017-02 /pmc/articles/PMC5324674/ /pubmed/27801963 http://dx.doi.org/10.1111/tpj.13409 Text en © 2016 The Authors. The Plant Journal published by John Wiley & Sons Ltd and Society for Experimental Biology. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Bastow, Emma L. Bych, Katrine Crack, Jason C. Le Brun, Nick E. Balk, Janneke NBP35 interacts with DRE2 in the maturation of cytosolic iron‐sulphur proteins in Arabidopsis thaliana |
title |
NBP35 interacts with DRE2 in the maturation of cytosolic iron‐sulphur proteins in Arabidopsis thaliana
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title_full |
NBP35 interacts with DRE2 in the maturation of cytosolic iron‐sulphur proteins in Arabidopsis thaliana
|
title_fullStr |
NBP35 interacts with DRE2 in the maturation of cytosolic iron‐sulphur proteins in Arabidopsis thaliana
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title_full_unstemmed |
NBP35 interacts with DRE2 in the maturation of cytosolic iron‐sulphur proteins in Arabidopsis thaliana
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title_short |
NBP35 interacts with DRE2 in the maturation of cytosolic iron‐sulphur proteins in Arabidopsis thaliana
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title_sort | nbp35 interacts with dre2 in the maturation of cytosolic iron‐sulphur proteins in arabidopsis thaliana |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5324674/ https://www.ncbi.nlm.nih.gov/pubmed/27801963 http://dx.doi.org/10.1111/tpj.13409 |
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