Active yeast ribosome preparation using monolithic anion exchange chromatography

In vitro studies of translation provide critical mechanistic details, yet purification of large amounts of highly active eukaryotic ribosomes remains a challenge for biochemists and structural biologists. Here, we present an optimized method for preparation of highly active yeast ribosomes that coul...

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Detalles Bibliográficos
Autores principales: Munoz, Antonio M., Yourik, Paul, Rajagopal, Vaishnavi, Nanda, Jagpreet S., Lorsch, Jon R., Walker, Sarah E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2016
Materias:
Technical Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5324736/
https://www.ncbi.nlm.nih.gov/pubmed/27981882
http://dx.doi.org/10.1080/15476286.2016.1270004

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5324736/
https://www.ncbi.nlm.nih.gov/pubmed/27981882
http://dx.doi.org/10.1080/15476286.2016.1270004

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