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Active yeast ribosome preparation using monolithic anion exchange chromatography

In vitro studies of translation provide critical mechanistic details, yet purification of large amounts of highly active eukaryotic ribosomes remains a challenge for biochemists and structural biologists. Here, we present an optimized method for preparation of highly active yeast ribosomes that coul...

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Detalles Bibliográficos
Autores principales:	Munoz, Antonio M., Yourik, Paul, Rajagopal, Vaishnavi, Nanda, Jagpreet S., Lorsch, Jon R., Walker, Sarah E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Taylor & Francis 2016
Materias:	Technical Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5324736/ https://www.ncbi.nlm.nih.gov/pubmed/27981882 http://dx.doi.org/10.1080/15476286.2016.1270004

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