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Livestock Susceptibility to Infection with Middle East Respiratory Syndrome Coronavirus
Middle East respiratory syndrome (MERS) cases continue to be reported, predominantly in Saudi Arabia and occasionally other countries. Although dromedaries are the main reservoir, other animal species might be susceptible to MERS coronavirus (MERS-CoV) infection and potentially serve as reservoirs....
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Centers for Disease Control and Prevention
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5324816/ https://www.ncbi.nlm.nih.gov/pubmed/27901465 http://dx.doi.org/10.3201/eid2302.161239 |
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author | Vergara-Alert, Júlia van den Brand, Judith M.A. Widagdo, W. Muñoz, Marta Raj, Stalin Schipper, Debby Solanes, David Cordón, Ivan Bensaid, Albert Haagmans, Bart L. Segalés, Joaquim |
author_facet | Vergara-Alert, Júlia van den Brand, Judith M.A. Widagdo, W. Muñoz, Marta Raj, Stalin Schipper, Debby Solanes, David Cordón, Ivan Bensaid, Albert Haagmans, Bart L. Segalés, Joaquim |
author_sort | Vergara-Alert, Júlia |
collection | PubMed |
description | Middle East respiratory syndrome (MERS) cases continue to be reported, predominantly in Saudi Arabia and occasionally other countries. Although dromedaries are the main reservoir, other animal species might be susceptible to MERS coronavirus (MERS-CoV) infection and potentially serve as reservoirs. To determine whether other animals are potential reservoirs, we inoculated MERS-CoV into llamas, pigs, sheep, and horses and collected nasal and rectal swab samples at various times. The presence of MERS-CoV in the nose of pigs and llamas was confirmed by PCR, titration of infectious virus, immunohistochemistry, and in situ hybridization; seroconversion was detected in animals of both species. Conversely, in sheep and horses, virus-specific antibodies did not develop and no evidence of viral replication in the upper respiratory tract was found. These results prove the susceptibility of llamas and pigs to MERS-CoV infection. Thus, the possibility of MERS-CoV circulation in animals other than dromedaries, such as llamas and pigs, is not negligible. |
format | Online Article Text |
id | pubmed-5324816 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Centers for Disease Control and Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-53248162017-02-24 Livestock Susceptibility to Infection with Middle East Respiratory Syndrome Coronavirus Vergara-Alert, Júlia van den Brand, Judith M.A. Widagdo, W. Muñoz, Marta Raj, Stalin Schipper, Debby Solanes, David Cordón, Ivan Bensaid, Albert Haagmans, Bart L. Segalés, Joaquim Emerg Infect Dis Research Middle East respiratory syndrome (MERS) cases continue to be reported, predominantly in Saudi Arabia and occasionally other countries. Although dromedaries are the main reservoir, other animal species might be susceptible to MERS coronavirus (MERS-CoV) infection and potentially serve as reservoirs. To determine whether other animals are potential reservoirs, we inoculated MERS-CoV into llamas, pigs, sheep, and horses and collected nasal and rectal swab samples at various times. The presence of MERS-CoV in the nose of pigs and llamas was confirmed by PCR, titration of infectious virus, immunohistochemistry, and in situ hybridization; seroconversion was detected in animals of both species. Conversely, in sheep and horses, virus-specific antibodies did not develop and no evidence of viral replication in the upper respiratory tract was found. These results prove the susceptibility of llamas and pigs to MERS-CoV infection. Thus, the possibility of MERS-CoV circulation in animals other than dromedaries, such as llamas and pigs, is not negligible. Centers for Disease Control and Prevention 2017-02 /pmc/articles/PMC5324816/ /pubmed/27901465 http://dx.doi.org/10.3201/eid2302.161239 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited. |
spellingShingle | Research Vergara-Alert, Júlia van den Brand, Judith M.A. Widagdo, W. Muñoz, Marta Raj, Stalin Schipper, Debby Solanes, David Cordón, Ivan Bensaid, Albert Haagmans, Bart L. Segalés, Joaquim Livestock Susceptibility to Infection with Middle East Respiratory Syndrome Coronavirus |
title | Livestock Susceptibility to Infection with Middle East Respiratory Syndrome Coronavirus |
title_full | Livestock Susceptibility to Infection with Middle East Respiratory Syndrome Coronavirus |
title_fullStr | Livestock Susceptibility to Infection with Middle East Respiratory Syndrome Coronavirus |
title_full_unstemmed | Livestock Susceptibility to Infection with Middle East Respiratory Syndrome Coronavirus |
title_short | Livestock Susceptibility to Infection with Middle East Respiratory Syndrome Coronavirus |
title_sort | livestock susceptibility to infection with middle east respiratory syndrome coronavirus |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5324816/ https://www.ncbi.nlm.nih.gov/pubmed/27901465 http://dx.doi.org/10.3201/eid2302.161239 |
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