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Significance of Glypican-3 (GPC3) Expression in Hepatocellular Cancer Diagnosis
BACKGROUND: Primary hepatocellular carcinoma (HCC) is a malignant tumor that is common China. Early diagnosis is of great significance for improving treatment efficiency. GPC3 level is closely related to HCC occurrence. This study investigated GPC3 expression in HCC patient serum and tissue, and ass...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325041/ https://www.ncbi.nlm.nih.gov/pubmed/28207681 http://dx.doi.org/10.12659/MSM.899198 |
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author | Sun, Bin Huang, Zhi Wang, Bi Yu, Yanglong Lin, Shihai Luo, Lei Wang, Yuzhu Huang, Zheng |
author_facet | Sun, Bin Huang, Zhi Wang, Bi Yu, Yanglong Lin, Shihai Luo, Lei Wang, Yuzhu Huang, Zheng |
author_sort | Sun, Bin |
collection | PubMed |
description | BACKGROUND: Primary hepatocellular carcinoma (HCC) is a malignant tumor that is common China. Early diagnosis is of great significance for improving treatment efficiency. GPC3 level is closely related to HCC occurrence. This study investigated GPC3 expression in HCC patient serum and tissue, and assessed the significance of GPC3 combined AFP detection in HCC diagnosis. MATERIAL/METHODS: A total of 76 HCC patients in our hospital were enrolled. Immunohistochemistry was applied to test GPC3 expression in cancer tissue and para-carcinoma tissue. ELISA and RT-PCR were used to detect GPC3 and AFP level in serum. The significance of GPC3 single or combined AFP detection in HCC diagnosis was analyzed. RESULTS: Immunohistochemistry showed that the GPC3 positive expression rate was obviously elevated in HCC tissue (P<0.01). Combination detection of AFP and GPC3 presented significantly higher sensitivity and specificity in HCC than single AFP or GPC3 detection. ELISA showed no significant difference in sensitivity, specificity, or accuracy compared with RT-PCR. CONCLUSIONS: Serum GPC3 was overexpressed in HCC patients. Combination detection of serum AFP and GPC3 can enhance accuracy and efficacy of HCC diagnosis. |
format | Online Article Text |
id | pubmed-5325041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53250412017-03-02 Significance of Glypican-3 (GPC3) Expression in Hepatocellular Cancer Diagnosis Sun, Bin Huang, Zhi Wang, Bi Yu, Yanglong Lin, Shihai Luo, Lei Wang, Yuzhu Huang, Zheng Med Sci Monit Clinical Research BACKGROUND: Primary hepatocellular carcinoma (HCC) is a malignant tumor that is common China. Early diagnosis is of great significance for improving treatment efficiency. GPC3 level is closely related to HCC occurrence. This study investigated GPC3 expression in HCC patient serum and tissue, and assessed the significance of GPC3 combined AFP detection in HCC diagnosis. MATERIAL/METHODS: A total of 76 HCC patients in our hospital were enrolled. Immunohistochemistry was applied to test GPC3 expression in cancer tissue and para-carcinoma tissue. ELISA and RT-PCR were used to detect GPC3 and AFP level in serum. The significance of GPC3 single or combined AFP detection in HCC diagnosis was analyzed. RESULTS: Immunohistochemistry showed that the GPC3 positive expression rate was obviously elevated in HCC tissue (P<0.01). Combination detection of AFP and GPC3 presented significantly higher sensitivity and specificity in HCC than single AFP or GPC3 detection. ELISA showed no significant difference in sensitivity, specificity, or accuracy compared with RT-PCR. CONCLUSIONS: Serum GPC3 was overexpressed in HCC patients. Combination detection of serum AFP and GPC3 can enhance accuracy and efficacy of HCC diagnosis. International Scientific Literature, Inc. 2017-02-16 /pmc/articles/PMC5325041/ /pubmed/28207681 http://dx.doi.org/10.12659/MSM.899198 Text en © Med Sci Monit, 2017 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) |
spellingShingle | Clinical Research Sun, Bin Huang, Zhi Wang, Bi Yu, Yanglong Lin, Shihai Luo, Lei Wang, Yuzhu Huang, Zheng Significance of Glypican-3 (GPC3) Expression in Hepatocellular Cancer Diagnosis |
title | Significance of Glypican-3 (GPC3) Expression in Hepatocellular Cancer Diagnosis |
title_full | Significance of Glypican-3 (GPC3) Expression in Hepatocellular Cancer Diagnosis |
title_fullStr | Significance of Glypican-3 (GPC3) Expression in Hepatocellular Cancer Diagnosis |
title_full_unstemmed | Significance of Glypican-3 (GPC3) Expression in Hepatocellular Cancer Diagnosis |
title_short | Significance of Glypican-3 (GPC3) Expression in Hepatocellular Cancer Diagnosis |
title_sort | significance of glypican-3 (gpc3) expression in hepatocellular cancer diagnosis |
topic | Clinical Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325041/ https://www.ncbi.nlm.nih.gov/pubmed/28207681 http://dx.doi.org/10.12659/MSM.899198 |
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