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Long noncoding RNA NEAT1 promotes cell proliferation and invasion by regulating hnRNP A2 expression in hepatocellular carcinoma cells

Growing evidence demonstrates that long noncoding RNAs (lncRNAs) are involved in the progression of various cancers, including hepatocellular carcinoma (HCC). The role of nuclear-enriched abundant transcript 1 (NEAT1), an essential lncRNA for the formation of nuclear body paraspeckles, has not been...

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Autores principales: Mang, Yuanyi, Li, Li, Ran, Jianghua, Zhang, Shengning, Liu, Jing, Li, Laibang, Chen, Yiming, Liu, Jian, Gao, Yang, Ren, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325106/
https://www.ncbi.nlm.nih.gov/pubmed/28260918
http://dx.doi.org/10.2147/OTT.S116319
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author Mang, Yuanyi
Li, Li
Ran, Jianghua
Zhang, Shengning
Liu, Jing
Li, Laibang
Chen, Yiming
Liu, Jian
Gao, Yang
Ren, Gang
author_facet Mang, Yuanyi
Li, Li
Ran, Jianghua
Zhang, Shengning
Liu, Jing
Li, Laibang
Chen, Yiming
Liu, Jian
Gao, Yang
Ren, Gang
author_sort Mang, Yuanyi
collection PubMed
description Growing evidence demonstrates that long noncoding RNAs (lncRNAs) are involved in the progression of various cancers, including hepatocellular carcinoma (HCC). The role of nuclear-enriched abundant transcript 1 (NEAT1), an essential lncRNA for the formation of nuclear body paraspeckles, has not been fully explored in HCC. We aimed to determine the expression, roles and functional mechanisms of NEAT1 in the proliferation and invasion of HCC. Based on real-time polymerase chain reaction data, we suggest that NEAT1 is upregulated in HCC tissues compared with noncancerous liver tissues. The knockdown of NEAT1 altered global gene expression patterns and reduced HCC cell proliferation, invasion and migration. RNA immunoprecipitation and RNA pull-down assays confirmed that U2AF65 binds to NEAT1. Furthermore, the study indicated that NEAT1 regulated hnRNP A2 expression and that this regulation may be associated with the NEAT1–U2AF65 protein complex. Thus, the NEAT1-hnRNP A2 regulation mechanism promotes HCC pathogenesis and may provide a potential target for the prognosis and treatment of HCC.
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spelling pubmed-53251062017-03-03 Long noncoding RNA NEAT1 promotes cell proliferation and invasion by regulating hnRNP A2 expression in hepatocellular carcinoma cells Mang, Yuanyi Li, Li Ran, Jianghua Zhang, Shengning Liu, Jing Li, Laibang Chen, Yiming Liu, Jian Gao, Yang Ren, Gang Onco Targets Ther Original Research Growing evidence demonstrates that long noncoding RNAs (lncRNAs) are involved in the progression of various cancers, including hepatocellular carcinoma (HCC). The role of nuclear-enriched abundant transcript 1 (NEAT1), an essential lncRNA for the formation of nuclear body paraspeckles, has not been fully explored in HCC. We aimed to determine the expression, roles and functional mechanisms of NEAT1 in the proliferation and invasion of HCC. Based on real-time polymerase chain reaction data, we suggest that NEAT1 is upregulated in HCC tissues compared with noncancerous liver tissues. The knockdown of NEAT1 altered global gene expression patterns and reduced HCC cell proliferation, invasion and migration. RNA immunoprecipitation and RNA pull-down assays confirmed that U2AF65 binds to NEAT1. Furthermore, the study indicated that NEAT1 regulated hnRNP A2 expression and that this regulation may be associated with the NEAT1–U2AF65 protein complex. Thus, the NEAT1-hnRNP A2 regulation mechanism promotes HCC pathogenesis and may provide a potential target for the prognosis and treatment of HCC. Dove Medical Press 2017-02-20 /pmc/articles/PMC5325106/ /pubmed/28260918 http://dx.doi.org/10.2147/OTT.S116319 Text en © 2017 Mang et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Mang, Yuanyi
Li, Li
Ran, Jianghua
Zhang, Shengning
Liu, Jing
Li, Laibang
Chen, Yiming
Liu, Jian
Gao, Yang
Ren, Gang
Long noncoding RNA NEAT1 promotes cell proliferation and invasion by regulating hnRNP A2 expression in hepatocellular carcinoma cells
title Long noncoding RNA NEAT1 promotes cell proliferation and invasion by regulating hnRNP A2 expression in hepatocellular carcinoma cells
title_full Long noncoding RNA NEAT1 promotes cell proliferation and invasion by regulating hnRNP A2 expression in hepatocellular carcinoma cells
title_fullStr Long noncoding RNA NEAT1 promotes cell proliferation and invasion by regulating hnRNP A2 expression in hepatocellular carcinoma cells
title_full_unstemmed Long noncoding RNA NEAT1 promotes cell proliferation and invasion by regulating hnRNP A2 expression in hepatocellular carcinoma cells
title_short Long noncoding RNA NEAT1 promotes cell proliferation and invasion by regulating hnRNP A2 expression in hepatocellular carcinoma cells
title_sort long noncoding rna neat1 promotes cell proliferation and invasion by regulating hnrnp a2 expression in hepatocellular carcinoma cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325106/
https://www.ncbi.nlm.nih.gov/pubmed/28260918
http://dx.doi.org/10.2147/OTT.S116319
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