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Mannose-coated gadolinium liposomes for improved magnetic resonance imaging in acute pancreatitis

BACKGROUND: Acute pancreatitis (AP) is an acute inflammatory condition of the pancreas. The symptoms, treatment, and prognosis of mild and severe AP are different, and severe AP is a potentially life-threatening disease with a high incidence of complications and high mortality rate. Thus, it is urge...

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Autores principales: Tian, Bing, Liu, Ri, Chen, Shiyue, Chen, Luguang, Liu, Fang, Jia, Guorong, Dong, Yinmei, Li, Jing, Chen, Huaiwen, Lu, Jianping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325132/
https://www.ncbi.nlm.nih.gov/pubmed/28260882
http://dx.doi.org/10.2147/IJN.S123290
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author Tian, Bing
Liu, Ri
Chen, Shiyue
Chen, Luguang
Liu, Fang
Jia, Guorong
Dong, Yinmei
Li, Jing
Chen, Huaiwen
Lu, Jianping
author_facet Tian, Bing
Liu, Ri
Chen, Shiyue
Chen, Luguang
Liu, Fang
Jia, Guorong
Dong, Yinmei
Li, Jing
Chen, Huaiwen
Lu, Jianping
author_sort Tian, Bing
collection PubMed
description BACKGROUND: Acute pancreatitis (AP) is an acute inflammatory condition of the pancreas. The symptoms, treatment, and prognosis of mild and severe AP are different, and severe AP is a potentially life-threatening disease with a high incidence of complications and high mortality rate. Thus, it is urgent to develop an effective approach to reliably discriminate between mild and severe AP. METHODS: We have developed novel gadolinium-diethylenetriaminepentaacetic (Gd-DTPA)-loaded mannosylated liposomes (named thereafter M-Gd-NL) that preferably target macrophages in AP. The targeting ability of M-Gd-NL toward macrophages in AP and its ability to discriminate between mild and severe AP were evaluated. RESULTS: The liposomes were of desired particle size (~100 nm), Gd-DTPA encapsulation efficiency (~85%), and stability. M-Gd-NL and non-targeted Gd-DTPA-loaded liposomes (Gd-NL) exhibited increased relaxivity compared with Gd-DTPA. Compared with Gd-NL and Gd-DTPA, M-Gd-NL showed increased uptake in macrophages, resulting in increased T(1) imaging ability both in vitro (macrophage cell line) and in vivo (severe AP model). Importantly, M-Gd-NL had the ability to discriminate between mild and severe AP, as reflected by a significantly higher T(1) magnetic resonance imaging signal in severe AP than in mild AP. M-Gd-NL did not show severe organ toxicity in rats. CONCLUSION: Our data suggest that M-Gd-NL had enhanced magnetic resonance imaging ability by targeting macrophages in AP and good ability to discriminate between mild and severe AP. We believe that M-Gd-NL could shed new light on the diagnosis of AP in the near future.
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spelling pubmed-53251322017-03-03 Mannose-coated gadolinium liposomes for improved magnetic resonance imaging in acute pancreatitis Tian, Bing Liu, Ri Chen, Shiyue Chen, Luguang Liu, Fang Jia, Guorong Dong, Yinmei Li, Jing Chen, Huaiwen Lu, Jianping Int J Nanomedicine Original Research BACKGROUND: Acute pancreatitis (AP) is an acute inflammatory condition of the pancreas. The symptoms, treatment, and prognosis of mild and severe AP are different, and severe AP is a potentially life-threatening disease with a high incidence of complications and high mortality rate. Thus, it is urgent to develop an effective approach to reliably discriminate between mild and severe AP. METHODS: We have developed novel gadolinium-diethylenetriaminepentaacetic (Gd-DTPA)-loaded mannosylated liposomes (named thereafter M-Gd-NL) that preferably target macrophages in AP. The targeting ability of M-Gd-NL toward macrophages in AP and its ability to discriminate between mild and severe AP were evaluated. RESULTS: The liposomes were of desired particle size (~100 nm), Gd-DTPA encapsulation efficiency (~85%), and stability. M-Gd-NL and non-targeted Gd-DTPA-loaded liposomes (Gd-NL) exhibited increased relaxivity compared with Gd-DTPA. Compared with Gd-NL and Gd-DTPA, M-Gd-NL showed increased uptake in macrophages, resulting in increased T(1) imaging ability both in vitro (macrophage cell line) and in vivo (severe AP model). Importantly, M-Gd-NL had the ability to discriminate between mild and severe AP, as reflected by a significantly higher T(1) magnetic resonance imaging signal in severe AP than in mild AP. M-Gd-NL did not show severe organ toxicity in rats. CONCLUSION: Our data suggest that M-Gd-NL had enhanced magnetic resonance imaging ability by targeting macrophages in AP and good ability to discriminate between mild and severe AP. We believe that M-Gd-NL could shed new light on the diagnosis of AP in the near future. Dove Medical Press 2017-02-13 /pmc/articles/PMC5325132/ /pubmed/28260882 http://dx.doi.org/10.2147/IJN.S123290 Text en © 2017 Tian et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Tian, Bing
Liu, Ri
Chen, Shiyue
Chen, Luguang
Liu, Fang
Jia, Guorong
Dong, Yinmei
Li, Jing
Chen, Huaiwen
Lu, Jianping
Mannose-coated gadolinium liposomes for improved magnetic resonance imaging in acute pancreatitis
title Mannose-coated gadolinium liposomes for improved magnetic resonance imaging in acute pancreatitis
title_full Mannose-coated gadolinium liposomes for improved magnetic resonance imaging in acute pancreatitis
title_fullStr Mannose-coated gadolinium liposomes for improved magnetic resonance imaging in acute pancreatitis
title_full_unstemmed Mannose-coated gadolinium liposomes for improved magnetic resonance imaging in acute pancreatitis
title_short Mannose-coated gadolinium liposomes for improved magnetic resonance imaging in acute pancreatitis
title_sort mannose-coated gadolinium liposomes for improved magnetic resonance imaging in acute pancreatitis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325132/
https://www.ncbi.nlm.nih.gov/pubmed/28260882
http://dx.doi.org/10.2147/IJN.S123290
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