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Long non-coding RNA HoxA-AS3 interacts with EZH2 to regulate lineage commitment of mesenchymal stem cells
Long non-coding RNAs (lncRNAs) play an important role in gene regulation and are involving in diverse cellular processes. However, their roles in reprogramming of gene expression profiles during lineage commitment and maturation of mesenchymal stem cells (MSCs) remain poorly understood. In the curre...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325385/ https://www.ncbi.nlm.nih.gov/pubmed/27566578 http://dx.doi.org/10.18632/oncotarget.11538 |
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author | Zhu, Xin-Xing Yan, Ya-Wei Chen, Demeng Ai, Chun-Zhi Lu, Xifeng Xu, Shan-Shan Jiang, Shan Zhong, Gen-Shen Chen, Dong-Bao Jiang, Yi-Zhou |
author_facet | Zhu, Xin-Xing Yan, Ya-Wei Chen, Demeng Ai, Chun-Zhi Lu, Xifeng Xu, Shan-Shan Jiang, Shan Zhong, Gen-Shen Chen, Dong-Bao Jiang, Yi-Zhou |
author_sort | Zhu, Xin-Xing |
collection | PubMed |
description | Long non-coding RNAs (lncRNAs) play an important role in gene regulation and are involving in diverse cellular processes. However, their roles in reprogramming of gene expression profiles during lineage commitment and maturation of mesenchymal stem cells (MSCs) remain poorly understood. In the current study, we characterize the expression of a lncRNA, HoxA-AS3, during the differentiation of MSCs. We showed that HoxA-AS3 is increased upon adipogenic induction of MSCs, while HoxA-AS3 remains unaltered during osteogenic induction. Silencing of HoxA-AS3 in MSCs resulted in decreased adipogenesis and expression of adipogenic markers, PPARG, CEBPA, FABP4 and ADIPOQ. Conversely, knockdown of HoxA-AS3 expression in MSCs exhibited an enhanced osteogenesis and osteogenic markers expression, including RUNX2, SP7, COL1A1, IBSP, BGLAP and SPP1. Mechanistically, HoxA-AS3 interacts with Enhancer Of Zeste 2 (EZH2) and is required for H3 lysine-27 trimethylation (H3K27me3) of key osteogenic transcription factor Runx2. Our data reveal that HoxA-AS3 acts as an epigenetic switch that determines the lineage specification of MSC. |
format | Online Article Text |
id | pubmed-5325385 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53253852017-03-23 Long non-coding RNA HoxA-AS3 interacts with EZH2 to regulate lineage commitment of mesenchymal stem cells Zhu, Xin-Xing Yan, Ya-Wei Chen, Demeng Ai, Chun-Zhi Lu, Xifeng Xu, Shan-Shan Jiang, Shan Zhong, Gen-Shen Chen, Dong-Bao Jiang, Yi-Zhou Oncotarget Research Paper Long non-coding RNAs (lncRNAs) play an important role in gene regulation and are involving in diverse cellular processes. However, their roles in reprogramming of gene expression profiles during lineage commitment and maturation of mesenchymal stem cells (MSCs) remain poorly understood. In the current study, we characterize the expression of a lncRNA, HoxA-AS3, during the differentiation of MSCs. We showed that HoxA-AS3 is increased upon adipogenic induction of MSCs, while HoxA-AS3 remains unaltered during osteogenic induction. Silencing of HoxA-AS3 in MSCs resulted in decreased adipogenesis and expression of adipogenic markers, PPARG, CEBPA, FABP4 and ADIPOQ. Conversely, knockdown of HoxA-AS3 expression in MSCs exhibited an enhanced osteogenesis and osteogenic markers expression, including RUNX2, SP7, COL1A1, IBSP, BGLAP and SPP1. Mechanistically, HoxA-AS3 interacts with Enhancer Of Zeste 2 (EZH2) and is required for H3 lysine-27 trimethylation (H3K27me3) of key osteogenic transcription factor Runx2. Our data reveal that HoxA-AS3 acts as an epigenetic switch that determines the lineage specification of MSC. Impact Journals LLC 2016-08-23 /pmc/articles/PMC5325385/ /pubmed/27566578 http://dx.doi.org/10.18632/oncotarget.11538 Text en Copyright: © 2016 Zhu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Zhu, Xin-Xing Yan, Ya-Wei Chen, Demeng Ai, Chun-Zhi Lu, Xifeng Xu, Shan-Shan Jiang, Shan Zhong, Gen-Shen Chen, Dong-Bao Jiang, Yi-Zhou Long non-coding RNA HoxA-AS3 interacts with EZH2 to regulate lineage commitment of mesenchymal stem cells |
title | Long non-coding RNA HoxA-AS3 interacts with EZH2 to regulate lineage commitment of mesenchymal stem cells |
title_full | Long non-coding RNA HoxA-AS3 interacts with EZH2 to regulate lineage commitment of mesenchymal stem cells |
title_fullStr | Long non-coding RNA HoxA-AS3 interacts with EZH2 to regulate lineage commitment of mesenchymal stem cells |
title_full_unstemmed | Long non-coding RNA HoxA-AS3 interacts with EZH2 to regulate lineage commitment of mesenchymal stem cells |
title_short | Long non-coding RNA HoxA-AS3 interacts with EZH2 to regulate lineage commitment of mesenchymal stem cells |
title_sort | long non-coding rna hoxa-as3 interacts with ezh2 to regulate lineage commitment of mesenchymal stem cells |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325385/ https://www.ncbi.nlm.nih.gov/pubmed/27566578 http://dx.doi.org/10.18632/oncotarget.11538 |
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