Cargando…

FAM122A, a new endogenous inhibitor of protein phosphatase 2A

The regulation of the ubiquitously expressed protein phosphatase 2A (PP2A) is essential for various cellular functions such as cell proliferation, transformation, and fate determination. In this study, we demonstrate that the highly conserved protein in mammals, designated FAM122A, directly interact...

Descripción completa

Detalles Bibliográficos
Autores principales: Fan, Li, Liu, Man-Hua, Guo, Meng, Hu, Chuan-Xi, Yan, Zhao-Wen, Chen, Jing, Chen, Guo-Qiang, Huang, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325411/
https://www.ncbi.nlm.nih.gov/pubmed/27588481
http://dx.doi.org/10.18632/oncotarget.11698
_version_ 1782510378432856064
author Fan, Li
Liu, Man-Hua
Guo, Meng
Hu, Chuan-Xi
Yan, Zhao-Wen
Chen, Jing
Chen, Guo-Qiang
Huang, Ying
author_facet Fan, Li
Liu, Man-Hua
Guo, Meng
Hu, Chuan-Xi
Yan, Zhao-Wen
Chen, Jing
Chen, Guo-Qiang
Huang, Ying
author_sort Fan, Li
collection PubMed
description The regulation of the ubiquitously expressed protein phosphatase 2A (PP2A) is essential for various cellular functions such as cell proliferation, transformation, and fate determination. In this study, we demonstrate that the highly conserved protein in mammals, designated FAM122A, directly interacts with PP2A-Aα and B55α rather than B56α subunits, and inhibits the phosphatase activity of PP2A-Aα/B55α/Cα complex. Further, FAM122A potentiates the degradation of catalytic subunit PP2A-Cα with the increased poly-ubiquitination. In agreement, FAM122A silencing inhibits while its overexpression enhances cell growth and colony-forming ability. Collectively, we identify FAM122A as a new endogenous PP2A inhibitor and its physiological and pathophysiological significances warrant to be further investigated.
format Online
Article
Text
id pubmed-5325411
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-53254112017-03-23 FAM122A, a new endogenous inhibitor of protein phosphatase 2A Fan, Li Liu, Man-Hua Guo, Meng Hu, Chuan-Xi Yan, Zhao-Wen Chen, Jing Chen, Guo-Qiang Huang, Ying Oncotarget Research Paper The regulation of the ubiquitously expressed protein phosphatase 2A (PP2A) is essential for various cellular functions such as cell proliferation, transformation, and fate determination. In this study, we demonstrate that the highly conserved protein in mammals, designated FAM122A, directly interacts with PP2A-Aα and B55α rather than B56α subunits, and inhibits the phosphatase activity of PP2A-Aα/B55α/Cα complex. Further, FAM122A potentiates the degradation of catalytic subunit PP2A-Cα with the increased poly-ubiquitination. In agreement, FAM122A silencing inhibits while its overexpression enhances cell growth and colony-forming ability. Collectively, we identify FAM122A as a new endogenous PP2A inhibitor and its physiological and pathophysiological significances warrant to be further investigated. Impact Journals LLC 2016-08-30 /pmc/articles/PMC5325411/ /pubmed/27588481 http://dx.doi.org/10.18632/oncotarget.11698 Text en Copyright: © 2016 Fan et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Fan, Li
Liu, Man-Hua
Guo, Meng
Hu, Chuan-Xi
Yan, Zhao-Wen
Chen, Jing
Chen, Guo-Qiang
Huang, Ying
FAM122A, a new endogenous inhibitor of protein phosphatase 2A
title FAM122A, a new endogenous inhibitor of protein phosphatase 2A
title_full FAM122A, a new endogenous inhibitor of protein phosphatase 2A
title_fullStr FAM122A, a new endogenous inhibitor of protein phosphatase 2A
title_full_unstemmed FAM122A, a new endogenous inhibitor of protein phosphatase 2A
title_short FAM122A, a new endogenous inhibitor of protein phosphatase 2A
title_sort fam122a, a new endogenous inhibitor of protein phosphatase 2a
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325411/
https://www.ncbi.nlm.nih.gov/pubmed/27588481
http://dx.doi.org/10.18632/oncotarget.11698
work_keys_str_mv AT fanli fam122aanewendogenousinhibitorofproteinphosphatase2a
AT liumanhua fam122aanewendogenousinhibitorofproteinphosphatase2a
AT guomeng fam122aanewendogenousinhibitorofproteinphosphatase2a
AT huchuanxi fam122aanewendogenousinhibitorofproteinphosphatase2a
AT yanzhaowen fam122aanewendogenousinhibitorofproteinphosphatase2a
AT chenjing fam122aanewendogenousinhibitorofproteinphosphatase2a
AT chenguoqiang fam122aanewendogenousinhibitorofproteinphosphatase2a
AT huangying fam122aanewendogenousinhibitorofproteinphosphatase2a