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Variabilité inter et intra-opérateur de l’analyse des paramètres spermatiques: résultat d’un programme de contrôle de qualité

INTRODUCTION: Semen analysis is a key part of male infertility investigation. The necessity of quality management implementation in the andrology laboratory has been recognized in order to ensure the reliability of its results. The aim of this study was to evaluate intra- and inter-individual variab...

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Autores principales: Daoud, Salima, Chakroun-Feki, Nozha, Sellami, Afifa, Ammar-Keskes, Leila, Rebai, Tarek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The African Field Epidemiology Network 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325489/
https://www.ncbi.nlm.nih.gov/pubmed/28292078
http://dx.doi.org/10.11604/pamj.2016.25.115.9158
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author Daoud, Salima
Chakroun-Feki, Nozha
Sellami, Afifa
Ammar-Keskes, Leila
Rebai, Tarek
author_facet Daoud, Salima
Chakroun-Feki, Nozha
Sellami, Afifa
Ammar-Keskes, Leila
Rebai, Tarek
author_sort Daoud, Salima
collection PubMed
description INTRODUCTION: Semen analysis is a key part of male infertility investigation. The necessity of quality management implementation in the andrology laboratory has been recognized in order to ensure the reliability of its results. The aim of this study was to evaluate intra- and inter-individual variability in the assessment of semen parameters in our laboratory through a quality control programme. METHODS: Four participants from the laboratory with different experience levels have participated in this study. Semen samples of varying quality were assessed for sperm motility, concentration and morphology and the results were used to evaluate inter-participant variability. In addition, replicates of each semen sample were analyzed to determine intra-individual variability for semen parameters analysis. RESULTS: The average values of inter-participant coefficients of variation for sperm motility, concentration and morphology were 12.8%, 19.8% and 48.9% respectively. The mean intra-participant coefficients of variation were, respectively, 6.9%, 12.3% and 42.7% for sperm motility, concentration and morphology. Despite some random errors of under- or overestimation, the overall results remained within the limits of acceptability for all participants. Sperm morphology assessment was particularly influenced by the participant’s level of experience. CONCLUSION: The present data emphasize the need for appropriate training of the laboratory staff and for regular participation in internal quality control programmes in order to improve the reliability of laboratory results.
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spelling pubmed-53254892017-03-10 Variabilité inter et intra-opérateur de l’analyse des paramètres spermatiques: résultat d’un programme de contrôle de qualité Daoud, Salima Chakroun-Feki, Nozha Sellami, Afifa Ammar-Keskes, Leila Rebai, Tarek Pan Afr Med J Research INTRODUCTION: Semen analysis is a key part of male infertility investigation. The necessity of quality management implementation in the andrology laboratory has been recognized in order to ensure the reliability of its results. The aim of this study was to evaluate intra- and inter-individual variability in the assessment of semen parameters in our laboratory through a quality control programme. METHODS: Four participants from the laboratory with different experience levels have participated in this study. Semen samples of varying quality were assessed for sperm motility, concentration and morphology and the results were used to evaluate inter-participant variability. In addition, replicates of each semen sample were analyzed to determine intra-individual variability for semen parameters analysis. RESULTS: The average values of inter-participant coefficients of variation for sperm motility, concentration and morphology were 12.8%, 19.8% and 48.9% respectively. The mean intra-participant coefficients of variation were, respectively, 6.9%, 12.3% and 42.7% for sperm motility, concentration and morphology. Despite some random errors of under- or overestimation, the overall results remained within the limits of acceptability for all participants. Sperm morphology assessment was particularly influenced by the participant’s level of experience. CONCLUSION: The present data emphasize the need for appropriate training of the laboratory staff and for regular participation in internal quality control programmes in order to improve the reliability of laboratory results. The African Field Epidemiology Network 2016-10-26 /pmc/articles/PMC5325489/ /pubmed/28292078 http://dx.doi.org/10.11604/pamj.2016.25.115.9158 Text en © Salima Daoud et al. http://creativecommons.org/licenses/by/2.0/ The Pan African Medical Journal - ISSN 1937-8688. This is an Open Access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Daoud, Salima
Chakroun-Feki, Nozha
Sellami, Afifa
Ammar-Keskes, Leila
Rebai, Tarek
Variabilité inter et intra-opérateur de l’analyse des paramètres spermatiques: résultat d’un programme de contrôle de qualité
title Variabilité inter et intra-opérateur de l’analyse des paramètres spermatiques: résultat d’un programme de contrôle de qualité
title_full Variabilité inter et intra-opérateur de l’analyse des paramètres spermatiques: résultat d’un programme de contrôle de qualité
title_fullStr Variabilité inter et intra-opérateur de l’analyse des paramètres spermatiques: résultat d’un programme de contrôle de qualité
title_full_unstemmed Variabilité inter et intra-opérateur de l’analyse des paramètres spermatiques: résultat d’un programme de contrôle de qualité
title_short Variabilité inter et intra-opérateur de l’analyse des paramètres spermatiques: résultat d’un programme de contrôle de qualité
title_sort variabilité inter et intra-opérateur de l’analyse des paramètres spermatiques: résultat d’un programme de contrôle de qualité
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325489/
https://www.ncbi.nlm.nih.gov/pubmed/28292078
http://dx.doi.org/10.11604/pamj.2016.25.115.9158
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