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Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance

A total of 54 clinical samples, including genital lesion swabs, whole blood and cerebrospinal fluid from patients diagnosed with syphilis were collected in 2006 and in 2013 in Buenos Aires, Argentina. Treponemal DNA was detected in 43 of the analyzed samples (79.6%) and further analyzed using Sequen...

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Autores principales: Gallo Vaulet, Lucía, Grillová, Linda, Mikalová, Lenka, Casco, Ricardo, Rodríguez Fermepin, Marcelo, Pando, María A., Šmajs, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325558/
https://www.ncbi.nlm.nih.gov/pubmed/28235102
http://dx.doi.org/10.1371/journal.pone.0172905
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author Gallo Vaulet, Lucía
Grillová, Linda
Mikalová, Lenka
Casco, Ricardo
Rodríguez Fermepin, Marcelo
Pando, María A.
Šmajs, David
author_facet Gallo Vaulet, Lucía
Grillová, Linda
Mikalová, Lenka
Casco, Ricardo
Rodríguez Fermepin, Marcelo
Pando, María A.
Šmajs, David
author_sort Gallo Vaulet, Lucía
collection PubMed
description A total of 54 clinical samples, including genital lesion swabs, whole blood and cerebrospinal fluid from patients diagnosed with syphilis were collected in 2006 and in 2013 in Buenos Aires, Argentina. Treponemal DNA was detected in 43 of the analyzed samples (79.6%) and further analyzed using Sequencing-based molecular typing (SBMT) and Enhanced CDC-typing (ECDCT). By SBMT, 10 different Treponema pallidum subsp. pallidum (TPA) genotypes were found, of which six were related to the TPA SS14 strain, and four to the TPA Nichols strain. The 23S rRNA gene was amplified in samples isolated from 42 patients, and in six of them (14.3%), either the A2058G (four patients, 9.5%) or the A2059G (two patients, 4.8%) mutations were found. In addition to Taiwan, Madagascar and Peru, Argentina is another country where the prevalence of Nichols-like isolates (26.8%) is greater than 10%.
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spelling pubmed-53255582017-03-09 Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance Gallo Vaulet, Lucía Grillová, Linda Mikalová, Lenka Casco, Ricardo Rodríguez Fermepin, Marcelo Pando, María A. Šmajs, David PLoS One Research Article A total of 54 clinical samples, including genital lesion swabs, whole blood and cerebrospinal fluid from patients diagnosed with syphilis were collected in 2006 and in 2013 in Buenos Aires, Argentina. Treponemal DNA was detected in 43 of the analyzed samples (79.6%) and further analyzed using Sequencing-based molecular typing (SBMT) and Enhanced CDC-typing (ECDCT). By SBMT, 10 different Treponema pallidum subsp. pallidum (TPA) genotypes were found, of which six were related to the TPA SS14 strain, and four to the TPA Nichols strain. The 23S rRNA gene was amplified in samples isolated from 42 patients, and in six of them (14.3%), either the A2058G (four patients, 9.5%) or the A2059G (two patients, 4.8%) mutations were found. In addition to Taiwan, Madagascar and Peru, Argentina is another country where the prevalence of Nichols-like isolates (26.8%) is greater than 10%. Public Library of Science 2017-02-24 /pmc/articles/PMC5325558/ /pubmed/28235102 http://dx.doi.org/10.1371/journal.pone.0172905 Text en © 2017 Gallo Vaulet et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gallo Vaulet, Lucía
Grillová, Linda
Mikalová, Lenka
Casco, Ricardo
Rodríguez Fermepin, Marcelo
Pando, María A.
Šmajs, David
Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance
title Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance
title_full Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance
title_fullStr Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance
title_full_unstemmed Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance
title_short Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance
title_sort molecular typing of treponema pallidum isolates from buenos aires, argentina: frequent nichols-like isolates and low levels of macrolide resistance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325558/
https://www.ncbi.nlm.nih.gov/pubmed/28235102
http://dx.doi.org/10.1371/journal.pone.0172905
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