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Changes in plasma protein levels as an early indication of a bloodstream infection
Blood culture is the primary diagnostic test performed in a suspicion of bloodstream infection to detect the presence of microorganisms and direct the treatment. However, blood culture is slow and time consuming method to detect blood stream infections or separate septic and/or bacteremic patients f...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325609/ https://www.ncbi.nlm.nih.gov/pubmed/28235076 http://dx.doi.org/10.1371/journal.pone.0172987 |
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author | Kuusela, Pentti Saraswat, Mayank Joenväärä, Sakari Kaartinen, Johanna Järvinen, Asko Renkonen, Risto |
author_facet | Kuusela, Pentti Saraswat, Mayank Joenväärä, Sakari Kaartinen, Johanna Järvinen, Asko Renkonen, Risto |
author_sort | Kuusela, Pentti |
collection | PubMed |
description | Blood culture is the primary diagnostic test performed in a suspicion of bloodstream infection to detect the presence of microorganisms and direct the treatment. However, blood culture is slow and time consuming method to detect blood stream infections or separate septic and/or bacteremic patients from others with less serious febrile disease. Plasma proteomics, despite its challenges, remains an important source for early biomarkers for systemic diseases and might show changes before direct evidence from bacteria can be obtained. We have performed a plasma proteomic analysis, simultaneously at the time of blood culture sampling from ten blood culture positive and ten blood culture negative patients, and quantified 172 proteins with two or more unique peptides. Principal components analysis, Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) and ROC curve analysis were performed to select protein(s) features which can classify the two groups of samples. We propose a number of candidates which qualify as potential biomarkers to select the blood culture positive cases from negative ones. Pathway analysis by two methods revealed complement activation, phagocytosis pathway and alterations in lipid metabolism as enriched pathways which are relevant for the condition. Data are available via ProteomeXchange with identifier PXD005022. |
format | Online Article Text |
id | pubmed-5325609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53256092017-03-09 Changes in plasma protein levels as an early indication of a bloodstream infection Kuusela, Pentti Saraswat, Mayank Joenväärä, Sakari Kaartinen, Johanna Järvinen, Asko Renkonen, Risto PLoS One Research Article Blood culture is the primary diagnostic test performed in a suspicion of bloodstream infection to detect the presence of microorganisms and direct the treatment. However, blood culture is slow and time consuming method to detect blood stream infections or separate septic and/or bacteremic patients from others with less serious febrile disease. Plasma proteomics, despite its challenges, remains an important source for early biomarkers for systemic diseases and might show changes before direct evidence from bacteria can be obtained. We have performed a plasma proteomic analysis, simultaneously at the time of blood culture sampling from ten blood culture positive and ten blood culture negative patients, and quantified 172 proteins with two or more unique peptides. Principal components analysis, Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) and ROC curve analysis were performed to select protein(s) features which can classify the two groups of samples. We propose a number of candidates which qualify as potential biomarkers to select the blood culture positive cases from negative ones. Pathway analysis by two methods revealed complement activation, phagocytosis pathway and alterations in lipid metabolism as enriched pathways which are relevant for the condition. Data are available via ProteomeXchange with identifier PXD005022. Public Library of Science 2017-02-24 /pmc/articles/PMC5325609/ /pubmed/28235076 http://dx.doi.org/10.1371/journal.pone.0172987 Text en © 2017 Kuusela et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kuusela, Pentti Saraswat, Mayank Joenväärä, Sakari Kaartinen, Johanna Järvinen, Asko Renkonen, Risto Changes in plasma protein levels as an early indication of a bloodstream infection |
title | Changes in plasma protein levels as an early indication of a bloodstream infection |
title_full | Changes in plasma protein levels as an early indication of a bloodstream infection |
title_fullStr | Changes in plasma protein levels as an early indication of a bloodstream infection |
title_full_unstemmed | Changes in plasma protein levels as an early indication of a bloodstream infection |
title_short | Changes in plasma protein levels as an early indication of a bloodstream infection |
title_sort | changes in plasma protein levels as an early indication of a bloodstream infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5325609/ https://www.ncbi.nlm.nih.gov/pubmed/28235076 http://dx.doi.org/10.1371/journal.pone.0172987 |
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