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Retrospective observation of drug susceptibility of Candida strains in the years 1999, 2004, and 2015

INTRODUCTION: There is much literature devoted to the problem of drug resistance and decreased susceptibility of fungi to commonly used antifungals. AIM: To analyze drug susceptibility of Candida albicans and non-Candida albicans strains isolated from the hands of people without any symptoms of dise...

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Autores principales: Łukaszuk, Cecylia, Krajewska-Kułak, Elżbieta, Kułak, Wojciech
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5326543/
https://www.ncbi.nlm.nih.gov/pubmed/28243540
http://dx.doi.org/10.7717/peerj.3038
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author Łukaszuk, Cecylia
Krajewska-Kułak, Elżbieta
Kułak, Wojciech
author_facet Łukaszuk, Cecylia
Krajewska-Kułak, Elżbieta
Kułak, Wojciech
author_sort Łukaszuk, Cecylia
collection PubMed
description INTRODUCTION: There is much literature devoted to the problem of drug resistance and decreased susceptibility of fungi to commonly used antifungals. AIM: To analyze drug susceptibility of Candida albicans and non-Candida albicans strains isolated from the hands of people without any symptoms of disease over a 16-year period. MATERIALS AND METHODS: The study included a total of 1,274 Candida-type strains isolated from the hands of people without any symptoms of disease, including: in 1999, 432 strains; in 2004, 368; and in 2015, 454 strains. Biological monitoring of hand surface contamination was performed using the Count-Tact(TM) applicator with Count-Tact plates (bioMerieux). Drug susceptibility was evaluated using FUNGITEST(®). RESULTS: In 1999, the most strains showed resistance to fluconazole (53.2%), in 2004 to itraconazole (52.9%), and in 2015 to fluconazole (85.8%). Resistance to more than one drug was 35.8% in 1999, 64.7% in 2004, and 92% in 2015. Mean resistance to azole antifungals significantly increased from 98 ± 39.7 strains in 1999 to 118.3 ± 29.6 in 2015 (p < 0.001). In 1999, the most strains showed resistance to fluconazole (50.6%), in 2004 to itraconazole (52.9%), and in 2015 to fluconazole (44.9%). Resistance to more than one drug was 52.9% in 1999, 64.3% in 2004, and 88.1% in 2015. Mean resistance to azole antifungals significantly increased from a mean of 76 ± 9.7 strains in 1999, to 95.3 ± 24.2 in 2004, and to 97.3 ± 16.6 in 2015 (p < 0.001). CONCLUSIONS: We showed increased C. albicans and non-Candida albicans strain resistance to commonly used antifungal chemotherapeutics, mainly imidazole. We found a clear rise in susceptibility of C. albicans and non-Candida albicans strains to several studied antifungals.
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spelling pubmed-53265432017-02-27 Retrospective observation of drug susceptibility of Candida strains in the years 1999, 2004, and 2015 Łukaszuk, Cecylia Krajewska-Kułak, Elżbieta Kułak, Wojciech PeerJ Microbiology INTRODUCTION: There is much literature devoted to the problem of drug resistance and decreased susceptibility of fungi to commonly used antifungals. AIM: To analyze drug susceptibility of Candida albicans and non-Candida albicans strains isolated from the hands of people without any symptoms of disease over a 16-year period. MATERIALS AND METHODS: The study included a total of 1,274 Candida-type strains isolated from the hands of people without any symptoms of disease, including: in 1999, 432 strains; in 2004, 368; and in 2015, 454 strains. Biological monitoring of hand surface contamination was performed using the Count-Tact(TM) applicator with Count-Tact plates (bioMerieux). Drug susceptibility was evaluated using FUNGITEST(®). RESULTS: In 1999, the most strains showed resistance to fluconazole (53.2%), in 2004 to itraconazole (52.9%), and in 2015 to fluconazole (85.8%). Resistance to more than one drug was 35.8% in 1999, 64.7% in 2004, and 92% in 2015. Mean resistance to azole antifungals significantly increased from 98 ± 39.7 strains in 1999 to 118.3 ± 29.6 in 2015 (p < 0.001). In 1999, the most strains showed resistance to fluconazole (50.6%), in 2004 to itraconazole (52.9%), and in 2015 to fluconazole (44.9%). Resistance to more than one drug was 52.9% in 1999, 64.3% in 2004, and 88.1% in 2015. Mean resistance to azole antifungals significantly increased from a mean of 76 ± 9.7 strains in 1999, to 95.3 ± 24.2 in 2004, and to 97.3 ± 16.6 in 2015 (p < 0.001). CONCLUSIONS: We showed increased C. albicans and non-Candida albicans strain resistance to commonly used antifungal chemotherapeutics, mainly imidazole. We found a clear rise in susceptibility of C. albicans and non-Candida albicans strains to several studied antifungals. PeerJ Inc. 2017-02-23 /pmc/articles/PMC5326543/ /pubmed/28243540 http://dx.doi.org/10.7717/peerj.3038 Text en ©2017 Łukaszuk et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Microbiology
Łukaszuk, Cecylia
Krajewska-Kułak, Elżbieta
Kułak, Wojciech
Retrospective observation of drug susceptibility of Candida strains in the years 1999, 2004, and 2015
title Retrospective observation of drug susceptibility of Candida strains in the years 1999, 2004, and 2015
title_full Retrospective observation of drug susceptibility of Candida strains in the years 1999, 2004, and 2015
title_fullStr Retrospective observation of drug susceptibility of Candida strains in the years 1999, 2004, and 2015
title_full_unstemmed Retrospective observation of drug susceptibility of Candida strains in the years 1999, 2004, and 2015
title_short Retrospective observation of drug susceptibility of Candida strains in the years 1999, 2004, and 2015
title_sort retrospective observation of drug susceptibility of candida strains in the years 1999, 2004, and 2015
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5326543/
https://www.ncbi.nlm.nih.gov/pubmed/28243540
http://dx.doi.org/10.7717/peerj.3038
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