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Simple platform for chronic imaging of hippocampal activity during spontaneous behaviour in an awake mouse

Chronic electrophysiological recordings of neuronal activity combined with two-photon Ca(2+) imaging give access to high resolution and cellular specificity. In addition, awake drug-free experimentation is required for investigating the physiological mechanisms that operate in the brain. Here, we de...

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Autores principales: Villette, Vincent, Levesque, Mathieu, Miled, Amine, Gosselin, Benoit, Topolnik, Lisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5327464/
https://www.ncbi.nlm.nih.gov/pubmed/28240275
http://dx.doi.org/10.1038/srep43388
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author Villette, Vincent
Levesque, Mathieu
Miled, Amine
Gosselin, Benoit
Topolnik, Lisa
author_facet Villette, Vincent
Levesque, Mathieu
Miled, Amine
Gosselin, Benoit
Topolnik, Lisa
author_sort Villette, Vincent
collection PubMed
description Chronic electrophysiological recordings of neuronal activity combined with two-photon Ca(2+) imaging give access to high resolution and cellular specificity. In addition, awake drug-free experimentation is required for investigating the physiological mechanisms that operate in the brain. Here, we developed a simple head fixation platform, which allows simultaneous chronic imaging and electrophysiological recordings to be obtained from the hippocampus of awake mice. We performed quantitative analyses of spontaneous animal behaviour, the associated network states and the cellular activities in the dorsal hippocampus as well as estimated the brain stability limits to image dendritic processes and individual axonal boutons. Ca(2+) imaging recordings revealed a relatively stereotyped hippocampal activity despite a high inter-animal and inter-day variability in the mouse behavior. In addition to quiet state and locomotion behavioural patterns, the platform allowed the reliable detection of walking steps and fine speed variations. The brain motion during locomotion was limited to ~1.8 μm, thus allowing for imaging of small sub-cellular structures to be performed in parallel with recordings of network and behavioural states. This simple device extends the drug-free experimentation in vivo, enabling high-stability optophysiological experiments with single-bouton resolution in the mouse awake brain.
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spelling pubmed-53274642017-03-03 Simple platform for chronic imaging of hippocampal activity during spontaneous behaviour in an awake mouse Villette, Vincent Levesque, Mathieu Miled, Amine Gosselin, Benoit Topolnik, Lisa Sci Rep Article Chronic electrophysiological recordings of neuronal activity combined with two-photon Ca(2+) imaging give access to high resolution and cellular specificity. In addition, awake drug-free experimentation is required for investigating the physiological mechanisms that operate in the brain. Here, we developed a simple head fixation platform, which allows simultaneous chronic imaging and electrophysiological recordings to be obtained from the hippocampus of awake mice. We performed quantitative analyses of spontaneous animal behaviour, the associated network states and the cellular activities in the dorsal hippocampus as well as estimated the brain stability limits to image dendritic processes and individual axonal boutons. Ca(2+) imaging recordings revealed a relatively stereotyped hippocampal activity despite a high inter-animal and inter-day variability in the mouse behavior. In addition to quiet state and locomotion behavioural patterns, the platform allowed the reliable detection of walking steps and fine speed variations. The brain motion during locomotion was limited to ~1.8 μm, thus allowing for imaging of small sub-cellular structures to be performed in parallel with recordings of network and behavioural states. This simple device extends the drug-free experimentation in vivo, enabling high-stability optophysiological experiments with single-bouton resolution in the mouse awake brain. Nature Publishing Group 2017-02-27 /pmc/articles/PMC5327464/ /pubmed/28240275 http://dx.doi.org/10.1038/srep43388 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Villette, Vincent
Levesque, Mathieu
Miled, Amine
Gosselin, Benoit
Topolnik, Lisa
Simple platform for chronic imaging of hippocampal activity during spontaneous behaviour in an awake mouse
title Simple platform for chronic imaging of hippocampal activity during spontaneous behaviour in an awake mouse
title_full Simple platform for chronic imaging of hippocampal activity during spontaneous behaviour in an awake mouse
title_fullStr Simple platform for chronic imaging of hippocampal activity during spontaneous behaviour in an awake mouse
title_full_unstemmed Simple platform for chronic imaging of hippocampal activity during spontaneous behaviour in an awake mouse
title_short Simple platform for chronic imaging of hippocampal activity during spontaneous behaviour in an awake mouse
title_sort simple platform for chronic imaging of hippocampal activity during spontaneous behaviour in an awake mouse
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5327464/
https://www.ncbi.nlm.nih.gov/pubmed/28240275
http://dx.doi.org/10.1038/srep43388
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