Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide

Separation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process. Graphene oxide adsorbs single-stranded DNA more quickly than double-stranded ones, allowing for selective removal of the former with a simple mixi...

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Detalles Bibliográficos
Autores principales: Huang, Po-Jung Jimmy, Liu, Juewen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5327888/
https://www.ncbi.nlm.nih.gov/pubmed/28348332
http://dx.doi.org/10.3390/nano3020221
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author Huang, Po-Jung Jimmy
Liu, Juewen
author_facet Huang, Po-Jung Jimmy
Liu, Juewen
author_sort Huang, Po-Jung Jimmy
collection PubMed
description Separation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process. Graphene oxide adsorbs single-stranded DNA more quickly than double-stranded ones, allowing for selective removal of the former with a simple mixing and centrifugation operation. The effect of DNA length and salt on adsorption selectivity has been characterized and its application in DNA melting curve measurement has been demonstrated.
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spelling pubmed-53278882017-03-21 Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide Huang, Po-Jung Jimmy Liu, Juewen Nanomaterials (Basel) Communication Separation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process. Graphene oxide adsorbs single-stranded DNA more quickly than double-stranded ones, allowing for selective removal of the former with a simple mixing and centrifugation operation. The effect of DNA length and salt on adsorption selectivity has been characterized and its application in DNA melting curve measurement has been demonstrated. MDPI 2013-04-04 /pmc/articles/PMC5327888/ /pubmed/28348332 http://dx.doi.org/10.3390/nano3020221 Text en © 2013 by the authors. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Communication
Huang, Po-Jung Jimmy
Liu, Juewen
Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide
title Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide
title_full Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide
title_fullStr Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide
title_full_unstemmed Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide
title_short Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide
title_sort separation of short single- and double-stranded dna based on their adsorption kinetics difference on graphene oxide
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5327888/
https://www.ncbi.nlm.nih.gov/pubmed/28348332
http://dx.doi.org/10.3390/nano3020221
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