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Gene expression-based biological test for major depressive disorder: an advanced study
PURPOSE: Recently, we could distinguished patients with major depressive disorder (MDD) from nonpsychiatric controls with high accuracy using a panel of five gene expression markers (ARHGAP24, HDAC5, PDGFC, PRNP, and SLC6A4) in leukocyte. In the present study, we examined whether this biological tes...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove Medical Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5328599/ https://www.ncbi.nlm.nih.gov/pubmed/28260899 http://dx.doi.org/10.2147/NDT.S120038 |
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author | Watanabe, Shin-ya Numata, Shusuke Iga, Jun-ichi Kinoshita, Makoto Umehara, Hidehiro Ishii, Kazuo Ohmori, Tetsuro |
author_facet | Watanabe, Shin-ya Numata, Shusuke Iga, Jun-ichi Kinoshita, Makoto Umehara, Hidehiro Ishii, Kazuo Ohmori, Tetsuro |
author_sort | Watanabe, Shin-ya |
collection | PubMed |
description | PURPOSE: Recently, we could distinguished patients with major depressive disorder (MDD) from nonpsychiatric controls with high accuracy using a panel of five gene expression markers (ARHGAP24, HDAC5, PDGFC, PRNP, and SLC6A4) in leukocyte. In the present study, we examined whether this biological test is able to discriminate patients with MDD from those without MDD, including those with schizophrenia and bipolar disorder. PATIENTS AND METHODS: We measured messenger ribonucleic acid expression levels of the aforementioned five genes in peripheral leukocytes in 17 patients with schizophrenia and 36 patients with bipolar disorder using quantitative real-time polymerase chain reaction (PCR), and we combined these expression data with our previous expression data of 25 patients with MDD and 25 controls. Subsequently, a linear discriminant function was developed for use in discriminating between patients with MDD and without MDD. RESULTS: This expression panel was able to segregate patients with MDD from those without MDD with a sensitivity and specificity of 64% and 67.9%, respectively. CONCLUSION: Further research to identify MDD-specific markers is needed to improve the performance of this biological test. |
format | Online Article Text |
id | pubmed-5328599 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-53285992017-03-03 Gene expression-based biological test for major depressive disorder: an advanced study Watanabe, Shin-ya Numata, Shusuke Iga, Jun-ichi Kinoshita, Makoto Umehara, Hidehiro Ishii, Kazuo Ohmori, Tetsuro Neuropsychiatr Dis Treat Original Research PURPOSE: Recently, we could distinguished patients with major depressive disorder (MDD) from nonpsychiatric controls with high accuracy using a panel of five gene expression markers (ARHGAP24, HDAC5, PDGFC, PRNP, and SLC6A4) in leukocyte. In the present study, we examined whether this biological test is able to discriminate patients with MDD from those without MDD, including those with schizophrenia and bipolar disorder. PATIENTS AND METHODS: We measured messenger ribonucleic acid expression levels of the aforementioned five genes in peripheral leukocytes in 17 patients with schizophrenia and 36 patients with bipolar disorder using quantitative real-time polymerase chain reaction (PCR), and we combined these expression data with our previous expression data of 25 patients with MDD and 25 controls. Subsequently, a linear discriminant function was developed for use in discriminating between patients with MDD and without MDD. RESULTS: This expression panel was able to segregate patients with MDD from those without MDD with a sensitivity and specificity of 64% and 67.9%, respectively. CONCLUSION: Further research to identify MDD-specific markers is needed to improve the performance of this biological test. Dove Medical Press 2017-02-21 /pmc/articles/PMC5328599/ /pubmed/28260899 http://dx.doi.org/10.2147/NDT.S120038 Text en © 2017 Watanabe et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Watanabe, Shin-ya Numata, Shusuke Iga, Jun-ichi Kinoshita, Makoto Umehara, Hidehiro Ishii, Kazuo Ohmori, Tetsuro Gene expression-based biological test for major depressive disorder: an advanced study |
title | Gene expression-based biological test for major depressive disorder: an advanced study |
title_full | Gene expression-based biological test for major depressive disorder: an advanced study |
title_fullStr | Gene expression-based biological test for major depressive disorder: an advanced study |
title_full_unstemmed | Gene expression-based biological test for major depressive disorder: an advanced study |
title_short | Gene expression-based biological test for major depressive disorder: an advanced study |
title_sort | gene expression-based biological test for major depressive disorder: an advanced study |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5328599/ https://www.ncbi.nlm.nih.gov/pubmed/28260899 http://dx.doi.org/10.2147/NDT.S120038 |
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