Cargando…
The ultrastructural organization of actin and myosin II filaments in the contractile ring: new support for an old model of cytokinesis
Despite recent advances in our understanding of the components and spatial regulation of the contractile ring (CR), the precise ultrastructure of actin and myosin II within the animal cell CR remains an unanswered question. We used superresolution light microscopy and platinum replica transmission e...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5328620/ https://www.ncbi.nlm.nih.gov/pubmed/28057763 http://dx.doi.org/10.1091/mbc.E16-06-0466 |
_version_ | 1782510916051402752 |
---|---|
author | Henson, John H. Ditzler, Casey E. Germain, Aphnie Irwin, Patrick M. Vogt, Eric T. Yang, Shucheng Wu, Xufeng Shuster, Charles B. |
author_facet | Henson, John H. Ditzler, Casey E. Germain, Aphnie Irwin, Patrick M. Vogt, Eric T. Yang, Shucheng Wu, Xufeng Shuster, Charles B. |
author_sort | Henson, John H. |
collection | PubMed |
description | Despite recent advances in our understanding of the components and spatial regulation of the contractile ring (CR), the precise ultrastructure of actin and myosin II within the animal cell CR remains an unanswered question. We used superresolution light microscopy and platinum replica transmission electron microscopy (TEM) to determine the structural organization of actin and myosin II in isolated cortical cytoskeletons prepared from dividing sea urchin embryos. Three-dimensional structured illumination microscopy indicated that within the CR, actin and myosin II filaments were organized into tightly packed linear arrays oriented along the axis of constriction and restricted to a narrow zone within the furrow. In contrast, myosin II filaments in earlier stages of cytokinesis were organized into small, discrete, and regularly spaced clusters. TEM showed that actin within the CR formed a dense and anisotropic array of elongate, antiparallel filaments, whereas myosin II was organized into laterally associated, head-to-head filament chains highly reminiscent of mammalian cell stress fibers. Together these results not only support the canonical “purse-string” model for contractile ring constriction, but also suggest that the CR may be derived from foci of myosin II filaments in a manner similar to what has been demonstrated in fission yeast. |
format | Online Article Text |
id | pubmed-5328620 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-53286202017-05-16 The ultrastructural organization of actin and myosin II filaments in the contractile ring: new support for an old model of cytokinesis Henson, John H. Ditzler, Casey E. Germain, Aphnie Irwin, Patrick M. Vogt, Eric T. Yang, Shucheng Wu, Xufeng Shuster, Charles B. Mol Biol Cell Articles Despite recent advances in our understanding of the components and spatial regulation of the contractile ring (CR), the precise ultrastructure of actin and myosin II within the animal cell CR remains an unanswered question. We used superresolution light microscopy and platinum replica transmission electron microscopy (TEM) to determine the structural organization of actin and myosin II in isolated cortical cytoskeletons prepared from dividing sea urchin embryos. Three-dimensional structured illumination microscopy indicated that within the CR, actin and myosin II filaments were organized into tightly packed linear arrays oriented along the axis of constriction and restricted to a narrow zone within the furrow. In contrast, myosin II filaments in earlier stages of cytokinesis were organized into small, discrete, and regularly spaced clusters. TEM showed that actin within the CR formed a dense and anisotropic array of elongate, antiparallel filaments, whereas myosin II was organized into laterally associated, head-to-head filament chains highly reminiscent of mammalian cell stress fibers. Together these results not only support the canonical “purse-string” model for contractile ring constriction, but also suggest that the CR may be derived from foci of myosin II filaments in a manner similar to what has been demonstrated in fission yeast. The American Society for Cell Biology 2017-03-01 /pmc/articles/PMC5328620/ /pubmed/28057763 http://dx.doi.org/10.1091/mbc.E16-06-0466 Text en © 2017 Henson et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Henson, John H. Ditzler, Casey E. Germain, Aphnie Irwin, Patrick M. Vogt, Eric T. Yang, Shucheng Wu, Xufeng Shuster, Charles B. The ultrastructural organization of actin and myosin II filaments in the contractile ring: new support for an old model of cytokinesis |
title | The ultrastructural organization of actin and myosin II filaments in the contractile ring: new support for an old model of cytokinesis |
title_full | The ultrastructural organization of actin and myosin II filaments in the contractile ring: new support for an old model of cytokinesis |
title_fullStr | The ultrastructural organization of actin and myosin II filaments in the contractile ring: new support for an old model of cytokinesis |
title_full_unstemmed | The ultrastructural organization of actin and myosin II filaments in the contractile ring: new support for an old model of cytokinesis |
title_short | The ultrastructural organization of actin and myosin II filaments in the contractile ring: new support for an old model of cytokinesis |
title_sort | ultrastructural organization of actin and myosin ii filaments in the contractile ring: new support for an old model of cytokinesis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5328620/ https://www.ncbi.nlm.nih.gov/pubmed/28057763 http://dx.doi.org/10.1091/mbc.E16-06-0466 |
work_keys_str_mv | AT hensonjohnh theultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis AT ditzlercaseye theultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis AT germainaphnie theultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis AT irwinpatrickm theultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis AT vogterict theultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis AT yangshucheng theultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis AT wuxufeng theultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis AT shustercharlesb theultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis AT hensonjohnh ultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis AT ditzlercaseye ultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis AT germainaphnie ultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis AT irwinpatrickm ultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis AT vogterict ultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis AT yangshucheng ultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis AT wuxufeng ultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis AT shustercharlesb ultrastructuralorganizationofactinandmyosiniifilamentsinthecontractileringnewsupportforanoldmodelofcytokinesis |