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Mechanism of nuclear movements in a multinucleated cell
Multinucleated cells are important in many organisms, but the mechanisms governing the movements of nuclei sharing a common cytoplasm are not understood. In the hyphae of the plant pathogenic fungus Ashbya gossypii, nuclei move back and forth, occasionally bypassing each other, preventing the format...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5328623/ https://www.ncbi.nlm.nih.gov/pubmed/28077618 http://dx.doi.org/10.1091/mbc.E16-11-0806 |
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author | Gibeaux, Romain Politi, Antonio Z. Philippsen, Peter Nédélec, François |
author_facet | Gibeaux, Romain Politi, Antonio Z. Philippsen, Peter Nédélec, François |
author_sort | Gibeaux, Romain |
collection | PubMed |
description | Multinucleated cells are important in many organisms, but the mechanisms governing the movements of nuclei sharing a common cytoplasm are not understood. In the hyphae of the plant pathogenic fungus Ashbya gossypii, nuclei move back and forth, occasionally bypassing each other, preventing the formation of nuclear clusters. This is essential for genetic stability. These movements depend on cytoplasmic microtubules emanating from the nuclei that are pulled by dynein motors anchored at the cortex. Using three-dimensional stochastic simulations with parameters constrained by the literature, we predict the cortical anchor density from the characteristics of nuclear movements. The model accounts for the complex nuclear movements seen in vivo, using a minimal set of experimentally determined ingredients. Of interest, these ingredients power the oscillations of the anaphase spindle in budding yeast, but in A. gossypii, this system is not restricted to a specific nuclear cycle stage, possibly as a result of adaptation to hyphal growth and multinuclearity. |
format | Online Article Text |
id | pubmed-5328623 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-53286232017-05-16 Mechanism of nuclear movements in a multinucleated cell Gibeaux, Romain Politi, Antonio Z. Philippsen, Peter Nédélec, François Mol Biol Cell Articles Multinucleated cells are important in many organisms, but the mechanisms governing the movements of nuclei sharing a common cytoplasm are not understood. In the hyphae of the plant pathogenic fungus Ashbya gossypii, nuclei move back and forth, occasionally bypassing each other, preventing the formation of nuclear clusters. This is essential for genetic stability. These movements depend on cytoplasmic microtubules emanating from the nuclei that are pulled by dynein motors anchored at the cortex. Using three-dimensional stochastic simulations with parameters constrained by the literature, we predict the cortical anchor density from the characteristics of nuclear movements. The model accounts for the complex nuclear movements seen in vivo, using a minimal set of experimentally determined ingredients. Of interest, these ingredients power the oscillations of the anaphase spindle in budding yeast, but in A. gossypii, this system is not restricted to a specific nuclear cycle stage, possibly as a result of adaptation to hyphal growth and multinuclearity. The American Society for Cell Biology 2017-03-01 /pmc/articles/PMC5328623/ /pubmed/28077618 http://dx.doi.org/10.1091/mbc.E16-11-0806 Text en © 2017 Gibeaux et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Gibeaux, Romain Politi, Antonio Z. Philippsen, Peter Nédélec, François Mechanism of nuclear movements in a multinucleated cell |
title | Mechanism of nuclear movements in a multinucleated cell |
title_full | Mechanism of nuclear movements in a multinucleated cell |
title_fullStr | Mechanism of nuclear movements in a multinucleated cell |
title_full_unstemmed | Mechanism of nuclear movements in a multinucleated cell |
title_short | Mechanism of nuclear movements in a multinucleated cell |
title_sort | mechanism of nuclear movements in a multinucleated cell |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5328623/ https://www.ncbi.nlm.nih.gov/pubmed/28077618 http://dx.doi.org/10.1091/mbc.E16-11-0806 |
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