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Lactobacillus johnsonii glycolipids, their structure and immunoreactivity with sera from inflammatory bowel disease patients

Structural studies of the major glycolipids produced by two Lactobacillus johnsonii (LJ) strains, LJ 151 isolated from intestinal tract of healthy mice and LJ 142 isolated from mice with experimentally induced inflammatory bowel disease (IBD), were performed. Two major glycolipids, GL1 and GL2, were...

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Detalles Bibliográficos
Autores principales: Paściak, Mariola, Górska, Sabina, Jawiarczyk, Natalia, Gamian, Andrzej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5328823/
https://www.ncbi.nlm.nih.gov/pubmed/27766756
http://dx.doi.org/10.1111/1751-7915.12424
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author Paściak, Mariola
Górska, Sabina
Jawiarczyk, Natalia
Gamian, Andrzej
author_facet Paściak, Mariola
Górska, Sabina
Jawiarczyk, Natalia
Gamian, Andrzej
author_sort Paściak, Mariola
collection PubMed
description Structural studies of the major glycolipids produced by two Lactobacillus johnsonii (LJ) strains, LJ 151 isolated from intestinal tract of healthy mice and LJ 142 isolated from mice with experimentally induced inflammatory bowel disease (IBD), were performed. Two major glycolipids, GL1 and GL2, were present in lipid extracts from L. johnsonii 142 and 151 strains. Glycolipid GL1 has been identified as β‐D‐Glcp‐(1→6)‐α‐D‐Galp‐(1→2)‐α‐D‐Glcp‐diglyceride and GL2 as α‐D‐Galp‐(1→2)‐α‐D‐Glcp‐diglyceride. The main fatty acid residues identified by gas‐liquid chromatography–mass spectrometry were palmitic, stearic and lactobacillic acids. Besides structural elucidation of the major glycolipids, the aim of this study was to determine the immunochemical properties of these glycolipids and to compare their immunoreactivity to that of polysaccharides obtained from the same strains. Sera from rabbits immunized with bacterial cells possessed much higher serological reactivity with polysaccharides than with glycolipids. Inversely, reactivity of the glycolipids with human sera from patients with IBD was much higher than that determined for the polysaccharides, while reactivity of glycolipids with human sera from healthy individuals was much lower than one measured for the polysaccharides. Results indicate that glycoconjugates from Lactobacillus cell wall act as antigens and may represent new IBD diagnostic biomarkers.
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spelling pubmed-53288232017-03-03 Lactobacillus johnsonii glycolipids, their structure and immunoreactivity with sera from inflammatory bowel disease patients Paściak, Mariola Górska, Sabina Jawiarczyk, Natalia Gamian, Andrzej Microb Biotechnol Research Articles Structural studies of the major glycolipids produced by two Lactobacillus johnsonii (LJ) strains, LJ 151 isolated from intestinal tract of healthy mice and LJ 142 isolated from mice with experimentally induced inflammatory bowel disease (IBD), were performed. Two major glycolipids, GL1 and GL2, were present in lipid extracts from L. johnsonii 142 and 151 strains. Glycolipid GL1 has been identified as β‐D‐Glcp‐(1→6)‐α‐D‐Galp‐(1→2)‐α‐D‐Glcp‐diglyceride and GL2 as α‐D‐Galp‐(1→2)‐α‐D‐Glcp‐diglyceride. The main fatty acid residues identified by gas‐liquid chromatography–mass spectrometry were palmitic, stearic and lactobacillic acids. Besides structural elucidation of the major glycolipids, the aim of this study was to determine the immunochemical properties of these glycolipids and to compare their immunoreactivity to that of polysaccharides obtained from the same strains. Sera from rabbits immunized with bacterial cells possessed much higher serological reactivity with polysaccharides than with glycolipids. Inversely, reactivity of the glycolipids with human sera from patients with IBD was much higher than that determined for the polysaccharides, while reactivity of glycolipids with human sera from healthy individuals was much lower than one measured for the polysaccharides. Results indicate that glycoconjugates from Lactobacillus cell wall act as antigens and may represent new IBD diagnostic biomarkers. John Wiley and Sons Inc. 2016-10-21 /pmc/articles/PMC5328823/ /pubmed/27766756 http://dx.doi.org/10.1111/1751-7915.12424 Text en © 2016 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Paściak, Mariola
Górska, Sabina
Jawiarczyk, Natalia
Gamian, Andrzej
Lactobacillus johnsonii glycolipids, their structure and immunoreactivity with sera from inflammatory bowel disease patients
title Lactobacillus johnsonii glycolipids, their structure and immunoreactivity with sera from inflammatory bowel disease patients
title_full Lactobacillus johnsonii glycolipids, their structure and immunoreactivity with sera from inflammatory bowel disease patients
title_fullStr Lactobacillus johnsonii glycolipids, their structure and immunoreactivity with sera from inflammatory bowel disease patients
title_full_unstemmed Lactobacillus johnsonii glycolipids, their structure and immunoreactivity with sera from inflammatory bowel disease patients
title_short Lactobacillus johnsonii glycolipids, their structure and immunoreactivity with sera from inflammatory bowel disease patients
title_sort lactobacillus johnsonii glycolipids, their structure and immunoreactivity with sera from inflammatory bowel disease patients
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5328823/
https://www.ncbi.nlm.nih.gov/pubmed/27766756
http://dx.doi.org/10.1111/1751-7915.12424
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