Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru

The complete bipartite genome (RNA1 and RNA2) of a new nepovirus infecting potato was obtained using small RNA sequencing and assembly complemented by Sanger sequencing. Each RNA encodes a single polyprotein, flanked by 5’ and 3’ untranslate regions (UTR) and followed by a poly (A) tail. The putativ...

Descripción completa

Detalles Bibliográficos
Autores principales: De Souza, Joao, Müller, Giovanna, Perez, Wilmer, Cuellar, Wilmer, Kreuze, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2016
Materias:
Annotated Sequence Record
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5329089/
https://www.ncbi.nlm.nih.gov/pubmed/27858290
http://dx.doi.org/10.1007/s00705-016-3147-6
_version_ 1782510989598523392
author De Souza, Joao
Müller, Giovanna
Perez, Wilmer
Cuellar, Wilmer
Kreuze, Jan
author_facet De Souza, Joao
Müller, Giovanna
Perez, Wilmer
Cuellar, Wilmer
Kreuze, Jan
author_sort De Souza, Joao
collection PubMed
description The complete bipartite genome (RNA1 and RNA2) of a new nepovirus infecting potato was obtained using small RNA sequencing and assembly complemented by Sanger sequencing. Each RNA encodes a single polyprotein, flanked by 5’ and 3’ untranslate regions (UTR) and followed by a poly (A) tail. The putative polyproteins encoded by RNA1 and RNA2 had sets of motifs which are characteristic of viruses in the genus Nepovirus. Sequence comparisons using the Pro-Pol region and the coat protein, including phylogenetic analysis of these regions, showed closest relationships with nepoviruses. The data obtained support the taxonomical status of this new virus (putative named Potato virus B, PVB) as a member of the genus Nepovirus, subgroup B. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00705-016-3147-6) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5329089
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Springer Vienna
record_format MEDLINE/PubMed
spelling pubmed-53290892017-03-13 Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru De Souza, Joao Müller, Giovanna Perez, Wilmer Cuellar, Wilmer Kreuze, Jan Arch Virol Annotated Sequence Record The complete bipartite genome (RNA1 and RNA2) of a new nepovirus infecting potato was obtained using small RNA sequencing and assembly complemented by Sanger sequencing. Each RNA encodes a single polyprotein, flanked by 5’ and 3’ untranslate regions (UTR) and followed by a poly (A) tail. The putative polyproteins encoded by RNA1 and RNA2 had sets of motifs which are characteristic of viruses in the genus Nepovirus. Sequence comparisons using the Pro-Pol region and the coat protein, including phylogenetic analysis of these regions, showed closest relationships with nepoviruses. The data obtained support the taxonomical status of this new virus (putative named Potato virus B, PVB) as a member of the genus Nepovirus, subgroup B. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00705-016-3147-6) contains supplementary material, which is available to authorized users. Springer Vienna 2016-11-17 2017 /pmc/articles/PMC5329089/ /pubmed/27858290 http://dx.doi.org/10.1007/s00705-016-3147-6 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Annotated Sequence Record
De Souza, Joao
Müller, Giovanna
Perez, Wilmer
Cuellar, Wilmer
Kreuze, Jan
Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru
title Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru
title_full Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru
title_fullStr Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru
title_full_unstemmed Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru
title_short Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru
title_sort complete sequence and variability of a new subgroup b nepovirus infecting potato in central peru
topic Annotated Sequence Record
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5329089/
https://www.ncbi.nlm.nih.gov/pubmed/27858290
http://dx.doi.org/10.1007/s00705-016-3147-6
work_keys_str_mv AT desouzajoao completesequenceandvariabilityofanewsubgroupbnepovirusinfectingpotatoincentralperu
AT mullergiovanna completesequenceandvariabilityofanewsubgroupbnepovirusinfectingpotatoincentralperu
AT perezwilmer completesequenceandvariabilityofanewsubgroupbnepovirusinfectingpotatoincentralperu
AT cuellarwilmer completesequenceandvariabilityofanewsubgroupbnepovirusinfectingpotatoincentralperu
AT kreuzejan completesequenceandvariabilityofanewsubgroupbnepovirusinfectingpotatoincentralperu

Ejemplares similares