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Antidiabetic Effects of Aqueous and Dichloromethane/Methanol Stem Bark Extracts of Pterocarpus soyauxii Taub (Papilionaceae) on Streptozotocin-induced Diabetic Rats

AIM OF THE STUDY: The aim is to evaluate the hypoglycemic and antidiabetic effects of aqueous and CH(2)Cl(2)/CH(3)OH stem bark extracts of Pterocarpus soyauxii Taub in normal and diabetic rats. MATERIALS AND METHODS: Streptozotocin (STZ)-induced diabetic and normal adult Wistar rats were orally admi...

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Autores principales: Tchamadeu, Marie Claire, Dzeufiet, Paul Désiré Djomeni, Blaes, Nelly, Girolami, Jean-Pierre, Kamtchouing, Pierre, Dimo, Théophile
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330109/
https://www.ncbi.nlm.nih.gov/pubmed/28250659
http://dx.doi.org/10.4103/0974-8490.199767
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author Tchamadeu, Marie Claire
Dzeufiet, Paul Désiré Djomeni
Blaes, Nelly
Girolami, Jean-Pierre
Kamtchouing, Pierre
Dimo, Théophile
author_facet Tchamadeu, Marie Claire
Dzeufiet, Paul Désiré Djomeni
Blaes, Nelly
Girolami, Jean-Pierre
Kamtchouing, Pierre
Dimo, Théophile
author_sort Tchamadeu, Marie Claire
collection PubMed
description AIM OF THE STUDY: The aim is to evaluate the hypoglycemic and antidiabetic effects of aqueous and CH(2)Cl(2)/CH(3)OH stem bark extracts of Pterocarpus soyauxii Taub in normal and diabetic rats. MATERIALS AND METHODS: Streptozotocin (STZ)-induced diabetic and normal adult Wistar rats were orally administered with aqueous and CH(2)Cl(2)/CH(3)OH plant extracts of P. soyauxii at various doses (38–300 mg/kg) in a single administration. In addition, STZ-induced diabetic rats received prolonged daily administration for 14 days. Glibenclamide (GB) (10 mg/kg) was used as reference treatment. In acute test, fasting blood glucose was followed for 5 h. In subacute test, body weight, food and water intakes, and blood glucose were followed weekly and serum biochemical parameters evaluated after 14 days treatment. RESULTS: Acute administration of aqueous and CH(2)Cl(2)/CH(3)OH stem bark extracts moderately decreased fasting blood glucose compared to GB, significantly in normal rats (P < 0.05 to P < 0.01) but, as GB, not significantly in diabetic rats. Prolonged treatments in diabetic rats with aqueous and CH(2)Cl(2)/CH(3)OH extracts reduced blood glucose to an extent, respectively, superior or similar to GB. Moreover, P. soyauxii also significantly (P < 0.01) reduced weight loss, and diabetes increased serum triglycerides, total cholesterol, and transaminases (alanine aminotransferase/aspartate aminotransferase) elevations. CONCLUSION: P. soyauxii Taub stem bark extracts have possible value for antidiabetic oral medication. SUMMARY: Aqueous and Dichloromethane/Methanol stem bark extracts of Pterocarpus soyauxii Taub have potent (compared to Glibenclamide) antidiabetic effects in STZ-diabetic rats, with specific kinetics and dose-responses. Moderate hypoglycemia effects upon acute P. soyauxii administration. Potent anti-hyperglycemic effects of sub-acute P. soyauxii administration in STZ-diabetic rats. Potent anti-hyperlipidemic effects of sub-acute P. soyauxii administration in STZ-diabetic rats. Improved hepatic and renal serum parameters after sub-acute P. soyauxii administration in STZ-diabetic rats. P. soyauxii extracts may be useful for oral treatment of diabetes and related metabolic disorders. [Image: see text] Abbreviations Used: CH2Cl2/CH3OH: Dichloromethane/Methanol; STZ: Streptozotocin; GB: Glibenclamide; AE: Aqueous extract; OE: Organic extract; FeCl3: Iron (III) chloride; NaCl: Sodium chloride; K3Fe(CN)6: Potassium ferricyanide; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; H: Hour; BW: Body weight, W1 and W2: Weeks 1 and 2; CHOD: Cholesterol oxidase; GPO: Glycerol-3 Phosphate oxidase; PAP: Phenol + Aminophenazone
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spelling pubmed-53301092017-03-01 Antidiabetic Effects of Aqueous and Dichloromethane/Methanol Stem Bark Extracts of Pterocarpus soyauxii Taub (Papilionaceae) on Streptozotocin-induced Diabetic Rats Tchamadeu, Marie Claire Dzeufiet, Paul Désiré Djomeni Blaes, Nelly Girolami, Jean-Pierre Kamtchouing, Pierre Dimo, Théophile Pharmacognosy Res Original Article AIM OF THE STUDY: The aim is to evaluate the hypoglycemic and antidiabetic effects of aqueous and CH(2)Cl(2)/CH(3)OH stem bark extracts of Pterocarpus soyauxii Taub in normal and diabetic rats. MATERIALS AND METHODS: Streptozotocin (STZ)-induced diabetic and normal adult Wistar rats were orally administered with aqueous and CH(2)Cl(2)/CH(3)OH plant extracts of P. soyauxii at various doses (38–300 mg/kg) in a single administration. In addition, STZ-induced diabetic rats received prolonged daily administration for 14 days. Glibenclamide (GB) (10 mg/kg) was used as reference treatment. In acute test, fasting blood glucose was followed for 5 h. In subacute test, body weight, food and water intakes, and blood glucose were followed weekly and serum biochemical parameters evaluated after 14 days treatment. RESULTS: Acute administration of aqueous and CH(2)Cl(2)/CH(3)OH stem bark extracts moderately decreased fasting blood glucose compared to GB, significantly in normal rats (P < 0.05 to P < 0.01) but, as GB, not significantly in diabetic rats. Prolonged treatments in diabetic rats with aqueous and CH(2)Cl(2)/CH(3)OH extracts reduced blood glucose to an extent, respectively, superior or similar to GB. Moreover, P. soyauxii also significantly (P < 0.01) reduced weight loss, and diabetes increased serum triglycerides, total cholesterol, and transaminases (alanine aminotransferase/aspartate aminotransferase) elevations. CONCLUSION: P. soyauxii Taub stem bark extracts have possible value for antidiabetic oral medication. SUMMARY: Aqueous and Dichloromethane/Methanol stem bark extracts of Pterocarpus soyauxii Taub have potent (compared to Glibenclamide) antidiabetic effects in STZ-diabetic rats, with specific kinetics and dose-responses. Moderate hypoglycemia effects upon acute P. soyauxii administration. Potent anti-hyperglycemic effects of sub-acute P. soyauxii administration in STZ-diabetic rats. Potent anti-hyperlipidemic effects of sub-acute P. soyauxii administration in STZ-diabetic rats. Improved hepatic and renal serum parameters after sub-acute P. soyauxii administration in STZ-diabetic rats. P. soyauxii extracts may be useful for oral treatment of diabetes and related metabolic disorders. [Image: see text] Abbreviations Used: CH2Cl2/CH3OH: Dichloromethane/Methanol; STZ: Streptozotocin; GB: Glibenclamide; AE: Aqueous extract; OE: Organic extract; FeCl3: Iron (III) chloride; NaCl: Sodium chloride; K3Fe(CN)6: Potassium ferricyanide; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; H: Hour; BW: Body weight, W1 and W2: Weeks 1 and 2; CHOD: Cholesterol oxidase; GPO: Glycerol-3 Phosphate oxidase; PAP: Phenol + Aminophenazone Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5330109/ /pubmed/28250659 http://dx.doi.org/10.4103/0974-8490.199767 Text en Copyright: © 2017 Pharmacognosy Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Tchamadeu, Marie Claire
Dzeufiet, Paul Désiré Djomeni
Blaes, Nelly
Girolami, Jean-Pierre
Kamtchouing, Pierre
Dimo, Théophile
Antidiabetic Effects of Aqueous and Dichloromethane/Methanol Stem Bark Extracts of Pterocarpus soyauxii Taub (Papilionaceae) on Streptozotocin-induced Diabetic Rats
title Antidiabetic Effects of Aqueous and Dichloromethane/Methanol Stem Bark Extracts of Pterocarpus soyauxii Taub (Papilionaceae) on Streptozotocin-induced Diabetic Rats
title_full Antidiabetic Effects of Aqueous and Dichloromethane/Methanol Stem Bark Extracts of Pterocarpus soyauxii Taub (Papilionaceae) on Streptozotocin-induced Diabetic Rats
title_fullStr Antidiabetic Effects of Aqueous and Dichloromethane/Methanol Stem Bark Extracts of Pterocarpus soyauxii Taub (Papilionaceae) on Streptozotocin-induced Diabetic Rats
title_full_unstemmed Antidiabetic Effects of Aqueous and Dichloromethane/Methanol Stem Bark Extracts of Pterocarpus soyauxii Taub (Papilionaceae) on Streptozotocin-induced Diabetic Rats
title_short Antidiabetic Effects of Aqueous and Dichloromethane/Methanol Stem Bark Extracts of Pterocarpus soyauxii Taub (Papilionaceae) on Streptozotocin-induced Diabetic Rats
title_sort antidiabetic effects of aqueous and dichloromethane/methanol stem bark extracts of pterocarpus soyauxii taub (papilionaceae) on streptozotocin-induced diabetic rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330109/
https://www.ncbi.nlm.nih.gov/pubmed/28250659
http://dx.doi.org/10.4103/0974-8490.199767
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